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Article Open Access

miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b

  • Authors:
    • Xujun Tang
    • Jiuxia Wang
    • Shuhong Zhou
    • Jing Zhou
    • Guyou Jia
    • Han Wang
    • Chunlei Xin
    • Guoning Fu
    • Jiahong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Osteoarthritis, Jining No. 2 People's Hospital, Jining, Shandong 272049, P.R. China, Department of Bone Oncology, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou, Gansu 730050, P.R. China, Department of Rheumatology, Gansu Provincial Hospital, Lanzhou, Gansu 730000, P.R. China, Department of Hematology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4843-4854
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    Published online on: October 29, 2019
       https://doi.org/10.3892/mmr.2019.10775
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Abstract

MicroRNAs serve an important role in the development of several diseases. Numerous genes regulate the skeletal muscle differentiation of C2C12 myoblasts. The role of miR‑760 in rheumatoid arthritis (RA) has not been reported, to the best of our knowledge. Therefore, the aim of the present study was to examine the role of miR‑760 in regulating skeletal muscle proliferation in RA. Potential genes functionally involved in the tarsal joint of a collagen‑induced RA model were identified using Gene Expression Omnibus. Reverse transcription‑quantitative PCR and western blot analyses were performed to determine the mRNA and protein expression levels. The proliferation, cell cycle progression and migration of C2C12 myoblasts were detected using Cell Counting Kit‑8, flow cytometry and wound‑healing assays, respectively. TargetScan was used to predict the potential target genes of miR‑760, and this was verified using a dual‑luciferase reporter assay. In the present study, myosin‑18b (Myo18b) expression was determined to be downregulated in the RA model. Silencing Myo18b decreased the proliferation, abrogated the cell cycle progression, and reduced the migration and differentiation of C2C12 myoblasts. Expression levels of cyclin‑dependent kinase 2, cyclin D1, matrix metalloproteinase (MMP)‑2, MMP‑9, myogenin and myosin heavy chain 6 were all decreased when Myo18b was silenced. Furthermore, overexpression of Myo18b induced opposing effects on C2C12 myoblasts. It was shown that Myo18b was a target gene of miRNA‑760. Overexpression of miR‑760 decreased proliferation, cell cycle progression, migration and differentiation in C2C12 myoblasts, and decreased the expression of Myo18b. The opposite results were observed when miR‑760 was downregulated. In conclusion, miR‑760 inhibited proliferation and differentiation by targeting Myo18b in C2C12 myoblasts. The results of the present study may contribute to understanding the mechanisms underlying RA skeletal muscle proliferation, and miR‑760/Myo18b may serve as potential targets for treating patients with RA.
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Copy and paste a formatted citation
Spandidos Publications style
Tang X, Wang J, Zhou S, Zhou J, Jia G, Wang H, Xin C, Fu G and Zhang J: miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b. Mol Med Rep 20: 4843-4854, 2019.
APA
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H. ... Zhang, J. (2019). miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b. Molecular Medicine Reports, 20, 4843-4854. https://doi.org/10.3892/mmr.2019.10775
MLA
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H., Xin, C., Fu, G., Zhang, J."miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b". Molecular Medicine Reports 20.6 (2019): 4843-4854.
Chicago
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H., Xin, C., Fu, G., Zhang, J."miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b". Molecular Medicine Reports 20, no. 6 (2019): 4843-4854. https://doi.org/10.3892/mmr.2019.10775
Copy and paste a formatted citation
x
Spandidos Publications style
Tang X, Wang J, Zhou S, Zhou J, Jia G, Wang H, Xin C, Fu G and Zhang J: miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b. Mol Med Rep 20: 4843-4854, 2019.
APA
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H. ... Zhang, J. (2019). miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b. Molecular Medicine Reports, 20, 4843-4854. https://doi.org/10.3892/mmr.2019.10775
MLA
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H., Xin, C., Fu, G., Zhang, J."miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b". Molecular Medicine Reports 20.6 (2019): 4843-4854.
Chicago
Tang, X., Wang, J., Zhou, S., Zhou, J., Jia, G., Wang, H., Xin, C., Fu, G., Zhang, J."miR‑760 regulates skeletal muscle proliferation in rheumatoid arthritis by targeting Myo18b". Molecular Medicine Reports 20, no. 6 (2019): 4843-4854. https://doi.org/10.3892/mmr.2019.10775
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