Altered Nurr1 protein expression in the hippocampal CA1 region following transient global cerebral ischemia

Park,Joon  Ha; Ahn,Ji  Hyeon; Kim,Dae  Won; Lee,Tae‑Kyeong; Park,Cheol  Woo; Park,Young  Eun; Lee,Jae‑Chul; Lee,Hyang‑Ah; Yang,Go  Eun; Won,Moo‑Ho; Lee,Choong‑Hyun

doi:10.3892/mmr.2019.10828

Altered Nurr1 protein expression in the hippocampal CA1 region following transient global cerebral ischemia

Authors:
- Joon Ha Park
- Ji Hyeon Ahn
- Dae Won Kim
- Tae‑Kyeong Lee
- Cheol Woo Park
- Young Eun Park
- Jae‑Chul Lee
- Hyang‑Ah Lee
- Go Eun Yang
- Moo‑Ho Won
- Choong‑Hyun Lee
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Affiliations: Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju, Gyeongbuk 38066, Republic of Korea, Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea, Department of Biochemistry and Molecular Biology, and Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Radiology, Kangwon National University Hospital, Chuncheon, Gangwon 24289, Republic of Korea, Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam‑do 31116, Republic of Korea
Published online on: November 20, 2019 https://doi.org/10.3892/mmr.2019.10828
Pages: 107-114
Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Nuclear receptor related 1 protein (Nurr1), a member of the nuclear receptor 4 family of orphan nuclear receptors, has been reported to display anti‑inflammatory properties. The present study investigated the alteration of Nurr1 immunoreactivity in the gerbil hippocampus proper following 5 min of transient global cerebral ischemia. In sham operated gerbils, Nurr1 immunoreactivity was observed in pyramidal neurons in all cornu ammonis 1‑3 (CA1‑3) subfields of the hippocampus proper. In ischemia‑operated gerbils, Nurr1 immunoreactivity was altered in the CA1 subfield. Nurr1 immunoreactivity in CA1 pyramidal neurons gradually decreased until 2 days post‑ischemia, and, at 4 days post‑ischemia, Nurr1 immunoreactivity was concentrated in CA1 pyramidal neurons. Additionally, Nurr1 immunoreactivity was newly expressed in microglia in the CA1 subfield at 4 days post‑ischemia. Conversely, in the CA2/3 subfield, time‑dependent alteration of Nurr1 immunoreactivity was not identified at any time following ischemia. These results indicated that the alteration of Nurr1 expression in the CA1 subfield in the hippocampus may be associated with the death of CA1 pyramidal neurons.

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