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Article Open Access

LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models

  • Authors:
    • Jianping Wu
    • Chuanguang Wang
    • Haiyang Ding
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesia, Lishui Municipal Central Hospital, Lishui, Zhejiang 323000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 291-303
    |
    Published online on: November 20, 2019
       https://doi.org/10.3892/mmr.2019.10829
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Abstract

The present study investigated the role and molecular mechanism of long non‑coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript (MALAT)1 in neuropathic pain in rat chronic constriction injury (CCI) model. Reverse transcription‑quantitative PCR and western blot analysis were used to detect the expression levels of MALAT1, microRNA (miR)‑154‑5p and aquaporin (AQP)9 in spinal cord tissue and microglia of CCI rats. ELISA and pain behavioral assays were used to observe the effect of MALAT1 on neuropathic pain and neuroinflammation in model rats, and to verify its molecular mechanism through bioinformatics and luciferase experiments. The results of the present study identified that the expression levels of MALAT1 and AQP9 were upregulated, while miR‑154‑5p was downregulated in spinal cord tissue and microglia of CCI rats. MALAT1 knockdown in CCI model rats significantly induced the occurrence of neuropathic pain, while the upregulation of miR‑154‑5p could reverse this process. The present study also identified that miR‑154‑5p was the target gene of MALAT1, and AQP9 was the target gene of miR‑154‑5p. AQP9 knockdown promoted the occurrence of neuropathic pain. In conclusion, lncRNA MALAT1 promotes the progression of neuropathic pain in rats by reducing miR‑154‑5p and increasing AQP9. The MALAT1/miR‑154‑5p/AQP9 axis can be used as a new therapeutic target for neuropathic pain.
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1 

Thomson S: Failed back surgery syndrome-definition, epidemiology and demographics. Br J Pain. 7:56–59. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Thomson S and Jacques L: Demographic characteristics of patients with severe neuropathic pain secondary to failed back surgery syndrome. Pain Pract. 9:206–215. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Liu XG, Pang RP, Zhou LJ, Wei XH and Zang Y: Neuropathic pain: Sensory nerve injury or motor nerve injury? Adv Exp Med Biol. 904:59–75. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Yalbuzdag SA, Erol AM, Sengul I, Celik C, Solum S, Adilay HU and Gungor B: Temperament and character profile in failed back surgery syndrome: A cross-sectional clinical study. Turk Neurosurg. 26:912–917. 2016.PubMed/NCBI

5 

Ellis A and Bennett DL: Neuroinflammation and the generation of neuropathic pain. Br J Anaesth. 111:26–37. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Yadav R and Weng HR: EZH2 regulates spinal neuroinflammation in rats with neuropathic pain. Neuroscience. 349:106–117. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Hauptman N and Glavac D: MicroRNAs and long non-coding RNAs: Prospects in diagnostics and therapy of cancer. Radiol Oncol. 47:311–318. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Morris KV and Mattick JS: The rise of regulatory RNA. Nat Rev Genet. 15:423–437. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Gu S, Xie R, Liu X, Shou J, Gu W and Che X: Long coding RNA XIST contributes to neuronal apoptosis through the downregulation of AKT phosphorylation and is negatively regulated by miR-494 in rat spinal cord injury. Int J Mol Sci. 18:E7322017. View Article : Google Scholar : PubMed/NCBI

10 

Li ZX, Zhu QN, Zhang HB, Hu Y, Wang G and Zhu YS: MALAT1: A potential biomarker in cancer. Cancer Manag Res. 10:6757–6768. 2018. View Article : Google Scholar : PubMed/NCBI

11 

Sun Y and Ma L: New insights into long non-coding RNA MALAT1 in cancer and metastasis. Cancers (Basel). 11:E2162019. View Article : Google Scholar : PubMed/NCBI

12 

Mei H and Liu Y, Zhou Q, Hu K and Liu Y: Long noncoding RNA MALAT1 acts as a potential biomarker in cancer diagnosis and detection: A meta-analysis. Biomark Med. 13:45–54. 2019. View Article : Google Scholar : PubMed/NCBI

13 

Xia C, Liang S, He Z, Zhu X, Chen R and Chen J: Metformin, a first-line drug for type 2 diabetes mellitus, disrupts the MALAT1/miR-142-3p sponge to decrease invasion and migration in cervical cancer cells. Eur J Pharmacol. 830:59–67. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Zhang Y, Wu H, Wang F, Ye M, Zhu H and Bu S: Long non-coding RNA MALAT1 expression in patients with gestational diabetes mellitus. Int J Gynaecol Obstet. 140:164–169. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Qiao Y, Peng C, Li J, Wu D and Wang X: LncRNA MALAT1 is neuroprotective in a rat model of spinal cord ischemia-reperfusion injury through miR-204 regulation. Curr Neurovasc Res. 15:211–219. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Xie X, Ma L, Xi K, Zhang W and Fan D: MicroRNA-183 suppresses neuropathic pain and expression of AMPA receptors by targeting mTOR/VEGF signaling pathway. Cell Physiol Biochem. 41:181–192. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Yang Z, Xu J, Zhu R and Liu L: Down-regulation of miRNA-128 contributes to neuropathic pain following spinal cord injury via activation of P38. Med Sci Monit. 23:405–411. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Zheng Y, Zhu C, Ma L, Shao P, Qin C, Li P, Cao Q, Ju X, Cheng G, Zhu Q, et al: miRNA-154-5p inhibits proliferation, migration and invasion by targeting E2F5 in prostate cancer cell lines. Urol Int. 98:102–110. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Wang X, Sun S, Tong X, Ma Q, Di H, Fu T, Sun Z, Cai Y, Fan W, Wu Q, et al: MiRNA-154-5p inhibits cell proliferation and metastasis by targeting PIWIL1 in glioblastoma. Brain Res. 1676:69–76. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Damiano A, Zotta E, Goldstein J, Reisin I and Ibarra C: Water channel proteins AQP3 and AQP9 are present in syncytiotrophoblast of human term placenta. Placenta. 22:776–781. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Jelen S, Parm Ulhøi B, Larsen A, Frøkiær J, Nielsen S and Rützler M: AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes. PLoS One. 8:e757642013. View Article : Google Scholar : PubMed/NCBI

22 

Lv Y, Huang Q, Dai W, Jie Y, Yu G, Fan X, Wu A and Miao Q: AQP9 promotes astrocytoma cell invasion and motility via the AKT pathway. Oncol Lett. 16:6059–6064. 2018.PubMed/NCBI

23 

Huang D, Feng X, Liu Y, Deng Y, Chen H, Chen D, Fang L, Cai Y, Liu H, Wang L, et al: AQP9-induced cell cycle arrest is associated with RAS activation and improves chemotherapy treatment efficacy in colorectal cancer. Cell Death Dis. 8:e28942017. View Article : Google Scholar : PubMed/NCBI

24 

Chung JM, Kim HK and Chung K: Segmental spinal nerve ligation model of neuropathic pain. Methods Mol Med. 99:35–45. 2004.PubMed/NCBI

25 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Guide for the Care and Use of Laboratory Animals. National Academies Press (US); Washington, DC: 2011

26 

Breivik H: International Association for the study of pain: Update on WHO-IASP activities. J Pain Symptom Manage. 24:97–101. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Beltramo M, Bernardini N, Bertorelli R, Campanella M, Nicolussi E, Fredduzzi S and Reggiani A: CB2 receptor-mediated antihyperalgesia: Possible direct involvement of neural mechanisms. Eur J Neurosci. 23:1530–1538. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Li JH, Liu S, Zhou H, Qu LH and Yang JH: StarBase v2.0: Decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res. 42:D92–D97. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Torrance N, Smith BH, Watson MC and Bennett MI: Medication and treatment use in primary care patients with chronic pain of predominantly neuropathic origin. Fam Pract. 24:481–485. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Torrance N, Smith BH, Bennett MI and Lee A: The epidemiology of chronic pain of predominantly neuropathic origin. Results from a general population survey. J Pain. 7:281–289. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Qiao W, Cao N and Yang L: MicroRNA-154 inhibits the growth and metastasis of gastric cancer cells by directly targeting MTDH. Oncol Lett. 14:3268–3274. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Xin C, Zhang H and Liu Z: miR-154 suppresses colorectal cancer cell growth and motility by targeting TLR2J. Mol Cell Biochem. 387:271–277. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Pang X, Huang K, Zhang Q, Zhang Y and Niu J: miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor. Oncol Rep. 34:3272–3279. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Halsey AM, Conner AC, Bill RM, Logan A and Ahmed Z: Aquaporins and their regulation after spinal cord injury. Cells. 7:E1742018. View Article : Google Scholar : PubMed/NCBI

36 

Nesic O, Lee J, Unabia GC, Johnson K, Ye Z, Vergara L, Hulsebosch CE and Perez-Polo JR: Aquaporin 1-a novel player in spinal cord injury. J Neurochem. 105:628–640. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu J, Wang C and Ding H: LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models. Mol Med Rep 21: 291-303, 2020.
APA
Wu, J., Wang, C., & Ding, H. (2020). LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models. Molecular Medicine Reports, 21, 291-303. https://doi.org/10.3892/mmr.2019.10829
MLA
Wu, J., Wang, C., Ding, H."LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models". Molecular Medicine Reports 21.1 (2020): 291-303.
Chicago
Wu, J., Wang, C., Ding, H."LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models". Molecular Medicine Reports 21, no. 1 (2020): 291-303. https://doi.org/10.3892/mmr.2019.10829
Copy and paste a formatted citation
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Spandidos Publications style
Wu J, Wang C and Ding H: LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models. Mol Med Rep 21: 291-303, 2020.
APA
Wu, J., Wang, C., & Ding, H. (2020). LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models. Molecular Medicine Reports, 21, 291-303. https://doi.org/10.3892/mmr.2019.10829
MLA
Wu, J., Wang, C., Ding, H."LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models". Molecular Medicine Reports 21.1 (2020): 291-303.
Chicago
Wu, J., Wang, C., Ding, H."LncRNA MALAT1 promotes neuropathic pain progression through the miR‑154‑5p/AQP9 axis in CCI rat models". Molecular Medicine Reports 21, no. 1 (2020): 291-303. https://doi.org/10.3892/mmr.2019.10829
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