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Article Open Access

Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells

  • Authors:
    • Qin Yu
    • Xiaoqin Xiong
    • Lei Zhao
    • Tingting Xu
    • Qianhua Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Wuhan Blood Center, Wuhan, Hubei 430000, P.R. China, Hubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, School of Chemistry and Life Sciences, Hubei University of Education, Wuhan, Hubei 430205, P.R. China, Department of Obstetrics and Gynecology, Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 181-190
    |
    Published online on: November 20, 2019
       https://doi.org/10.3892/mmr.2019.10834
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Abstract

Connective tissue growth factor (CTGF) is a possible key determinant of progressive fibrosis. Nanotechnology has been considered as a potential tool for developing novel drug delivery systems for various diseases, including liver fibrosis. The present study aimed to investigate the potential antifibrotic activity of CTGF small interfering RNA (siRNA) mediated by polyethyleneimine (PEI)‑functionalized magnetic iron oxide (Fe3O4) nanoparticles (NPs) in LX‑2 cells. PEI‑Fe3O4/siRNA complexes were synthesized to facilitate siRNA delivery and were transfected into LX‑2 cells. Laser confocal microscopy was employed to investigate the cell uptake of PEI‑Fe3O4/siRNA complexes. Reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting were used to verify the effect of gene silencing. The results showed that siRNA‑loaded PEI‑Fe3O4 exhibited low cytotoxicity. The transfection efficiency of PEI‑Fe3O4/siRNA reached 73.8%, and RT‑qPCR and western blotting demonstrated effective gene silencing. These results indicated that CTGF siRNA delivered by PEI‑Fe3O4 NPs significantly reduces CTGF expression and collagen production in activated LX‑2 cells, providing a basis for future in vivo studies.
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View References

1 

Atta HM: Reversibility and heritability of liver fibrosis: Implications for research and therapy. World J Gastroenterol. 21:5138–5148. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Kisseleva T and Brenner DA: Hepatic stellate cells and the reversal of fibrosis. J Gastroenterol Hepatol. 21 (Suppl 3):S84–S87. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Gressner OA and Gressner AM: Connective tissue growth factor: A fibrogenic master switch in fibrotic liver diseases. Liver Int. 28:1065–1079. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Salazar-Montes AM, Hernández-Ortega LD, Lucano-Landeros MS and Armendariz-Borunda J: New gene therapy strategies for hepatic fibrosis. World J Gastroenterol. 21:3813–3825. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Gonzalez-Rodriguez A and Valverde AM: RNA interference as a therapeutic strategy for the treatment of liver diseases. Curr Pharm Des. 21:4574–4586. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Cheng K, Yang N and Mahato RI: TGF-beta1 gene silencing for treating liver fibrosis. Mol Pharm. 6:772–779. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Omar R, Yang J, Liu H, Davies NM and Gong Y: Hepatic stellate cells in liver fibrosis and siRNA-based therapy. Rev Physiol Biochem Pharmacol. 172:1–37. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Motoyama H, Ogawa S, Kubo A, Miwa S, Nakayama J, Tagawa Y and Miyagawa S: In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors. Biochem Biophys Res Commun. 385:123–128. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Zhou J, Shum KT, Burnett JC and Rossi JJ: Nanoparticle-based delivery of RNAi therapeutics: Progress and challenges. Pharmaceuticals (Basel). 6:85–107. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Poilil Surendran SP, Thomas RG, Moon MJ and Jeong YY: Nanoparticles for the treatment of liver fibrosis. Int J Nanomedicine. 12:6997–7006. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Li TT, Shen X, Chen Y, Zhang CC, Yan J, Yang H, Wu CH, Zeng HJ and Liu YY: Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging. Int J Nanomedicine. 10:4279–4291. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Shirazi AN, Paquin KL, Howlett NG, Mandal D and Parang K: Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery. Molecules. 19:13319–13331. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Wang Y, Zhao Q, Han N, Bai L, Li J, Liu J, Che E, Hu L, Zhang Q, Jiang T and Wang S: Mesoporous silica nanoparticles in drug delivery and biomedical applications. Nanomedicine. 11:313–327. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Reina G, Gismondi A, Carcione R, Nanni V, Peruzzi C, Angjellari M, Chau NDQ, Canini A, Terranova ML and Tamburri E: Oxidized and amino-functionalized nanodiamonds as shuttle for delivery of plant secondary metabolites: Interplay between chemical affinity and bioactivity. Appl Surface Sci. 470:744–754. 2019. View Article : Google Scholar

15 

Getz T, Qin J, Medintz IL, Delehanty JB, Susumu K, Dawson PE and Dawson G: Quantum dot-mediated delivery of siRNA to inhibit sphingomyelinase activities in brain-derived cells. J Neurochem. 139:872–885. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Gupta AK and Gupta M: Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials. 26:3995–4021. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Wu W, He QG and Jiang CZ: Magnetic iron oxide nanoparticles: Synthesis and surface functionalization strategies. Nanoscale Res Lett. 3:397–415. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Xiao S, Castro R, Rodrigues J, Shi X and Tomás H: PAMAM dendrimer/pDNA functionalized-magnetic iron oxide nanoparticles for gene delivery. J Biomed Nanotechnol. 11:1370–1384. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Saeed M, Ren W and Wu A: Therapeutic applications of iron oxide based nanoparticles in cancer: Basic concepts and recent advances. Biomater Sci. 6:708–725. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Li J, Zou S, Gao J, Liang J, Zhou H, Liang L and Wu W: Block copolymer conjugated Au-coated Fe3O4 nanoparticles as vectors for enhancing colloidal stability and cellular uptake. J Nanobiotechnology. 15:562017. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Friedman SL: Cytokines and fibrogenesis. Semin Liver Dis. 19:129–140. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Bataller R and Brenner DA: Liver fibrosis. J Clin Invest. 115:209–218. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Elpek GÖ: Cellular and molecular mechanisms in the pathogenesis of liver fibrosis: An update. World J Gastroenterol. 20:7260–7276. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Trautwein C, Friedman SL, Schuppan D and Pinzani M: Hepatic fibrosis: Concept to treatment. J Hepatol. 62 (1 Suppl):S15–S24. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Zhang CY, Yuan WG, He P, Lei JH and Wang CX: Liver fibrosis and hepatic stellate cells: Etiology, pathological hallmarks and therapeutic targets. World J Gastroenterol. 22:10512–10522. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Schuppan D, Surabattula R and Wang XY: Determinants of fibrosis progression and regression in NASH. J Hepatol. 68:238–250. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Lipson KE, Wong C, Teng Y and Spong S: CTGF is a central mediator of tissue remodeling and fibrosis and its inhibition can reverse the process of fibrosis. Fibrogenesis Tissue Repair. 5 (Suppl 1):S242012. View Article : Google Scholar : PubMed/NCBI

29 

Hao C, Xie Y, Peng M, Ma L, Zhou Y, Zhang Y, Kang W, Wang J, Bai X, Wang P and Jia Z: Inhibition of connective tissue growth factor suppresses hepatic stellate cell activation in vitro and prevents liver fibrosis in vivo. Clin Exp Med. 14:141–150. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Li G, Xie Q, Shi Y, Li D, Zhang M, Jiang S, Zhou H, Lu H and Jin Y: Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. J Gene Med. 8:889–900. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Taymouri S and Taheri A: Use of nanotechnology in diagnosis and treatment of hepatic fibrosis: A review. Curr Drug Deliv. 13:662–672. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 98:503–517. 1975. View Article : Google Scholar : PubMed/NCBI

33 

Wu J and Zern MA: Hepatic stellate cells: A target for the treatment of liver fibrosis. J Gastroenterol. 35:665–672. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Gressner AM, Weiskirchen R, Breitkopf K and Dooley S: Roles of TGF-beta in hepatic fibrosis. Front Biosci. 7:d793–d807. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Yang N, Dang S, Shi J, Wu F, Li M, Zhang X, Li Y, Jia X and Zhai S: Caffeic acid phenethyl ester attenuates liver fibrosis via inhibition of TGF-β1/Smad3 pathway and induction of autophagy pathway. Biochem Biophys Res Commun. 486:22–28. 2017. View Article : Google Scholar : PubMed/NCBI

36 

Lee UE and Friedman SL: Mechanisms of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol. 25:195–206. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Huang G and Brigstock DR: Regulation of hepatic stellate cells by connective tissue growth factor. Front Biosci (Landmark Ed). 17:2495–2507. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Yuhua Z, Wanhua R, Chenggang S, Jun S, Yanjun W and Chunqing Z: Disruption of connective tissue growth factor by short hairpin RNA inhibits collagen synthesis and extracellular matrix secretion in hepatic stellate cells. Liver Int. 28:632–639. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu Q, Xiong X, Zhao L, Xu T and Wang Q: Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells. Mol Med Rep 21: 181-190, 2020.
APA
Yu, Q., Xiong, X., Zhao, L., Xu, T., & Wang, Q. (2020). Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells. Molecular Medicine Reports, 21, 181-190. https://doi.org/10.3892/mmr.2019.10834
MLA
Yu, Q., Xiong, X., Zhao, L., Xu, T., Wang, Q."Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells". Molecular Medicine Reports 21.1 (2020): 181-190.
Chicago
Yu, Q., Xiong, X., Zhao, L., Xu, T., Wang, Q."Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells". Molecular Medicine Reports 21, no. 1 (2020): 181-190. https://doi.org/10.3892/mmr.2019.10834
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Q, Xiong X, Zhao L, Xu T and Wang Q: Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells. Mol Med Rep 21: 181-190, 2020.
APA
Yu, Q., Xiong, X., Zhao, L., Xu, T., & Wang, Q. (2020). Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells. Molecular Medicine Reports, 21, 181-190. https://doi.org/10.3892/mmr.2019.10834
MLA
Yu, Q., Xiong, X., Zhao, L., Xu, T., Wang, Q."Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells". Molecular Medicine Reports 21.1 (2020): 181-190.
Chicago
Yu, Q., Xiong, X., Zhao, L., Xu, T., Wang, Q."Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells". Molecular Medicine Reports 21, no. 1 (2020): 181-190. https://doi.org/10.3892/mmr.2019.10834
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