miR‑337‑3p inhibits gastric tumor metastasis by targeting ARHGAP10

Wang,Zishu; Yao,Lun; Li,Yu; Hao,Bo; Wang,Mingxi; Wang,Junbin; Gu,Wei; Zhan,Huihui; Liu,Guoquan; Wu,Qiong

doi:10.3892/mmr.2019.10856

miR‑337‑3p inhibits gastric tumor metastasis by targeting ARHGAP10

Authors:
- Zishu Wang
- Lun Yao
- Yu Li
- Bo Hao
- Mingxi Wang
- Junbin Wang
- Wei Gu
- Huihui Zhan
- Guoquan Liu
- Qiong Wu
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Affiliations: Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical College, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu, Anhui 233004, P.R. China, Department of Basic Veterinary Medicine, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China
Published online on: November 29, 2019 https://doi.org/10.3892/mmr.2019.10856
Pages: 705-719
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Several microRNAs (miRNAs) are known as regulatory molecules involved in gastric tumor metastasis. The expression of miR‑337‑3p was revealed to be downregulated in metastatic gastric tumor cells. Overexpression of miR‑337‑3p in gastric cancer cells resulted in the reduction of their invasive abilities. To characterize the functions of miR‑337‑3p, miR‑337‑3p was expressed in a metastatic lymph node‑derived gastric tumor cell line, SGC‑7901. Overexpression of miR‑337‑3p reduced the viability of cells but had no effects on the cell cycle. Wound healing and Transwell migration assays revealed that miR‑337‑3p inhibited the migration capacity of cells. miR‑337‑3p was capable of binding to the 3'‑untranslated region of a cytoskeleton‑associated molecule, ARHGAP10. Overexpression of miR‑337‑3p reduced the mRNA and protein levels of ARHGAP10 and the co‑expression of ARHGAP10 and miR‑337‑3p resulted in the recovery of cell migration capacity. Furthermore, the injection of miR‑337‑3p‑overexpressing SGC‑7901 cells into an immunodeficient mouse model resulted in a decrease in tumor metastasis in the liver and lungs. The present results indicated that miR‑337‑3p regulates gastric tumor metastasis by targeting the cytoskeleton‑associated protein ARHGAP10.

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