Phellinus gilvus‑derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16‑F10 cells
- Shi Zhong
- Qinshen Jin
- Taihen Yu
- Jianxun Zhu
- Yougui Li
Affiliations: Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, P.R. China, Nanxun District Agricultural Technology Extension Service Center, Huzhou, Zhejiang 313009, P.R. China, Department of Radiology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, P.R. China
- Published online on: December 19, 2019 https://doi.org/10.3892/mmr.2019.10896
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Protocatechualdehyde (PCA) is considered to be the main phenolic component of Phellinus gilvus responsible for its anticancer properties. Previous studies have demonstrated that PCA can have an anticancer effect on multiple cancer types, but little is known about the effect of PCA on melanoma cells. The present study investigated the inhibitory abilities and potential anticancer mechanisms of PCA on B16‑F10 cells using MTT assay. Cell apoptosis and cell cycle were assessed by flow cytometry using Annexin V‑FITC and propidium iodide staining. Whole‑transcriptome analysis was used to investigate the effects of PCA on gene expression. PCA significantly decreased cell viability, induced cell cycle arrest at G0/G1 phase and promoted apoptosis of B16‑F10 cells, suggesting that PCA could have anticancer effects against melanoma cells. Whole‑transcriptome analysis indicated that PCA treatment upregulated genes involved in histone modification and decreased the transcription of genes involved in DNA repair and replication. Kyoto Encyclopedia of Genes and Genomes analysis showed that PCA treatment enhanced the complement and coagulation cascades, and the p53 signaling pathway. The present results indicated that PCA could act as an antitumor agent in melanoma cells, which may provide experimental support for the development of novel therapies to treat melanoma.