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Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells

  • Authors:
    • Yabin Yu
    • Meng Li
    • Yan Song
    • Jianbo Xu
    • Fuzhen Qi
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu 223300, P.R. China, Department of Pathology, Xuzhou Medical University, Xuzhou, Jiangsu 221004, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1051-1058
    |
    Published online on: December 19, 2019
       https://doi.org/10.3892/mmr.2019.10897
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Abstract

Previous studies have shown that long noncoding RNAs (lncRNAs) are capable of regulating cell differentiation and pluripotency. The objective of the present study was to explore the effect of lncRNA cancer upregulated drug resistant (CUDR) on the hepatic differentiation of human umbilical cord mesenchymal stem cells (HuMSCs). HuMSCs were subjected to a hepatogenic differentiation protocol. The level of CUDR was monitored by reverse transcription‑quantitative PCR (RT‑qPCR) following certain stages of hepatic differentiation. Lentivirus transfection was used to achieve CUDR overexpression. The hepatocyte‑related proteins and mRNAs were then examined by immunofluorescence, ELISA and RT‑qPCR analyses. The results showed that CUDR was upregulated during the hepatic differentiation of HuMSCs. Upregulation of CUDR can improve hepatic differentiation of HuMSCs, including hepatocyte‑related genes and proteins. In addition, it was also found that liver‑enriched transcription factors were upregulated after CUDR overexpression. Moreover, there was an association between the Wnt/β‑catenin pathway and CUDR. In summary, these results demonstrated that the overexpression of CUDR could improve the hepatic differentiation of HuMSCs, therefore it could be an ideal source for regenerative therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Li M, Song Y, Xu J and Qi F: Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells. Mol Med Rep 21: 1051-1058, 2020.
APA
Yu, Y., Li, M., Song, Y., Xu, J., & Qi, F. (2020). Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells. Molecular Medicine Reports, 21, 1051-1058. https://doi.org/10.3892/mmr.2019.10897
MLA
Yu, Y., Li, M., Song, Y., Xu, J., Qi, F."Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells". Molecular Medicine Reports 21.3 (2020): 1051-1058.
Chicago
Yu, Y., Li, M., Song, Y., Xu, J., Qi, F."Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells". Molecular Medicine Reports 21, no. 3 (2020): 1051-1058. https://doi.org/10.3892/mmr.2019.10897
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Li M, Song Y, Xu J and Qi F: Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells. Mol Med Rep 21: 1051-1058, 2020.
APA
Yu, Y., Li, M., Song, Y., Xu, J., & Qi, F. (2020). Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells. Molecular Medicine Reports, 21, 1051-1058. https://doi.org/10.3892/mmr.2019.10897
MLA
Yu, Y., Li, M., Song, Y., Xu, J., Qi, F."Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells". Molecular Medicine Reports 21.3 (2020): 1051-1058.
Chicago
Yu, Y., Li, M., Song, Y., Xu, J., Qi, F."Overexpression of long noncoding RNA CUDR promotes hepatic differentiation of human umbilical cord mesenchymal stem cells". Molecular Medicine Reports 21, no. 3 (2020): 1051-1058. https://doi.org/10.3892/mmr.2019.10897
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