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‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach

  • Authors:
    • Wiesława Kranc
    • Maciej Brązert
    • Piotr Celichowski
    • Artur Bryja
    • Mariusz J. Nawrocki
    • Katarzyna Ożegowska
    • Maurycy Jankowski
    • Michal Jeseta
    • Leszek Pawelczyk
    • Andrzej Bręborowicz
    • Dominik Rachoń
    • Mariusz T. Skowroński
    • Małgorzata Bruska
    • Maciej Zabel
    • Michał Nowicki
    • Bartosz Kempisty
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Poznan University of Medical Sciences, 60‑781 Poznań, Poland, Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, 60‑535 Poznań, Poland, Department of Histology and Embryology, Poznan University of Medical Sciences, 60‑781 Poznań, Poland, Department of Obstetrics and Gynecology, University Hospital and Masaryk University, 62500 Brno, Czech Republic, Department of Pathophysiology, Poznań University of Medical Sciences, 60‑806 Poznań, Poland, Department of Clinical and Experimental Endocrinology, Medical University of Gdańsk, 80‑211 Gdańsk, Poland, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10‑719 Olsztyn, Poland, Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50‑368 Wroclaw, Poland
    Copyright: © Kranc et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1705-1715
    |
    Published online on: January 8, 2019
       https://doi.org/10.3892/mmr.2019.9837
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Abstract

Granulosa cells (GCs) have many functions in the endocrine system. Most notably, they produce progesterone following ovulation. However, it has recently been proven that GCs can change their properties when subjected to long‑term culture. In the present study, GCs were collected from hyper‑stimulated ovarian follicles during in vitro fertilization procedures. They were grown in vitro, in a long‑term manner. RNA was collected following 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In addition, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to validate the obtained results. Two ontological groups characteristic for processes associated with the development and morphogenesis of the heart were identified during the analyses: ‘Heart development’ and ‘heart morphogenesis’. The results of the microarrays revealed that the highest change in expression was demonstrated by the lysyl Oxidase, oxytocin receptor, nexilin F‑actin binding protein, and cysteine‑rich protein 3 genes. The lowest change was exhibited by odd‑skipped related transcription factor 1, plakophilin 2, transcription growth factor‑β receptor 1, and kinesin family member 3A. The direction of changes was confirmed by RT‑qPCR results. In the present study, it was suggested that GCs may have the potential to differentiate towards other cell types under long‑term in vitro culture conditions. Thus, genes belonging to the presented ontological groups can be considered as novel markers of proliferation and differentiation of GCs towards the heart muscle cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kranc W, Brązert M, Celichowski P, Bryja A, Nawrocki MJ, Ożegowska K, Jankowski M, Jeseta M, Pawelczyk L, Bręborowicz A, Bręborowicz A, et al: ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Mol Med Rep 19: 1705-1715, 2019.
APA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M.J., Ożegowska, K. ... Kempisty, B. (2019). ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Molecular Medicine Reports, 19, 1705-1715. https://doi.org/10.3892/mmr.2019.9837
MLA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19.3 (2019): 1705-1715.
Chicago
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19, no. 3 (2019): 1705-1715. https://doi.org/10.3892/mmr.2019.9837
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Spandidos Publications style
Kranc W, Brązert M, Celichowski P, Bryja A, Nawrocki MJ, Ożegowska K, Jankowski M, Jeseta M, Pawelczyk L, Bręborowicz A, Bręborowicz A, et al: ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Mol Med Rep 19: 1705-1715, 2019.
APA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M.J., Ożegowska, K. ... Kempisty, B. (2019). ‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach. Molecular Medicine Reports, 19, 1705-1715. https://doi.org/10.3892/mmr.2019.9837
MLA
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19.3 (2019): 1705-1715.
Chicago
Kranc, W., Brązert, M., Celichowski, P., Bryja, A., Nawrocki, M. J., Ożegowska, K., Jankowski, M., Jeseta, M., Pawelczyk, L., Bręborowicz, A., Rachoń, D., Skowroński, M. T., Bruska, M., Zabel, M., Nowicki, M., Kempisty, B."‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach". Molecular Medicine Reports 19, no. 3 (2019): 1705-1715. https://doi.org/10.3892/mmr.2019.9837
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