Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2019 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2019 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use

  • Authors:
    • Yin‑Di Wu
    • Meng Li
    • Xuan Liao
    • Sheng‑Hong Li
    • Jian‑Xin Yan
    • Lei Fan
    • Wen‑Li She
    • Jian‑Xin Song
    • Hong‑Wei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Guangzhou, Guangdong 510630, P.R. China, Department of Orthopedics, Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Plastic Surgery, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2189-2201
    |
    Published online on: January 10, 2019
       https://doi.org/10.3892/mmr.2019.9842
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Adipose‑derived stem cells (ADSCs) are mesenchymal stem cells that are often used in regenerative medicine. Maintaining ADSC viability is important, as this optimizes the curative effects of cell therapy. However, the optimal conditions for cell viability preservation remain unknown. The present study aimed to acquire a better protocol for ADSC storage by comparing the effects of various solutions and temperatures for ADSC preservation, in order to suggest the most effective methods of short‑term ADSC preservation for clinical use. ADSCs from passage 2 were suspended in solutions comprising 0.9% NaCl, 10% human serum (HS) or 10% platelet‑rich plasma (PRP). Suspended cells were maintained at 4˚C or room temperature (~26˚C) for 2, 4 and 6 h. The differentiation capacity, apoptosis and proliferation of ADSCs were determined by oil red O/alizarin red S staining, flow cytometry, and a cell counting kit‑8 cell proliferation assay, respectively. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot analysis was performed. The results revealed that proliferation of ADSCs decreased with time. The optimal time for ADSC use was ~2 h, and 4 h was determined to be the latest time that ADSCs should be used. The 10% HS group had the highest survival rate, followed by the 10% PRP group; these two groups had higher survival rates than the 0.9% NaCl group (P<0.05). HS and PRP at 4˚C enhanced the ADSC proliferation rate (P<0.05), although the difference between these two groups was insignificant (P>0.05). In conclusion, the optimal time to use ADSCs was <2 h, and should not exceed 4 h. It was recommended that, for the transportation and short‑term storage of ADSCs during clinical use, they should be stored with 10% HS at 4˚C to maintain ADSC viability. In addition, this was a cost‑effective and safe method.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Gimble JM, Katz AJ and Bunnell BA: Adipose-derived stem cell for regenerative medicine. Circ Res. 100:1249–1260. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Bora P and Majumdar AS: Adipose tissue-derived stromal vascular fraction in regenerative medicine: A brief review on biology and translation. Stem Cell Res Ther. 8:1452017. View Article : Google Scholar : PubMed/NCBI

3 

Gimble JM: Adipose tissue-derived therapeutics. Expert Opin Biol Ther. 3:705–713. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Baer PC and Geiger H: Adipose-derived mesenchymal stromal/stem cell: Tissue localization, characterization, and heterogeneity. Stem Cells Int 2012. 8126932012.

5 

Qiu X, Fandel TM, Ferretti L, Albersen M, Orabi H, Zhang H, Lin G, Lin CS, Schroeder T and Lue TF: Both immediate and delayed intracavernous injection of autologous adipose-derived stromal vascular fraction enhances recovery of erectile function in a rat model of cavernous nerve injury. Eur Urol. 62:720–727. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Lin G, Garcia M, Ning H, Banie L, Guo YL, Lue TF and Lin CS: Defining stem and progenitor cell within adipose tissue. Stem Cells Dev. 17:1053–1063. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Tobita M, Orbay H and Mizuno H: Adipose-derived stem cell: Current findings and future perspectives. Discov Med. 11:160–170. 2011.PubMed/NCBI

8 

Gonda K, Shigeura T, Sato T, Matsumoto D, Suga H, Inoue K, Aoi N, Kato H, Sato K, Murase S, et al: Preserved proliferative capacity and multipotency of human adipose-derived stem cell after long-term cryopreservation. Plast Reconstr Surg. 121:401–410. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Gonzalez-Fernandez ML, Perez-Castrillo S, Ordas-Fernandez P, Lopez-Gonzalez ME, Colaco B and Villar-Suarez V: Study on viability and chondrogenic differentiation of cryopreserved adipose tissue-derived mesenchymal stromal cell for future use in regenerative medicine. Cryobiology. 71:256–263. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Matsumoto D, Shigeura T, Sato K, Inoue K, Suga H, Kato H, Aoi N, Murase S, Gonda K and Yoshimura K: Influences of preservation at various temperatures on liposuction aspirates. Plast Reconstr Surg. 120:1510–1517. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Saragusty J and Arav A: Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification. Reproduction. 141:1–19. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Saragusty J, Gacitua H, Rozenboim I and Arav A: Protective effects of iodixanol during bovine sperm cryopreservation. Theriogenology. 71:1425–1432. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Bunnell BA, Flaat M, Gagliardi C, Patel B and Ripoll C: Adipose-derived stem cell: Isolation, expansion and differentiation. Methods. 45:115–120. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Zhang F, Ren H, Shao X, Zhuang C, Chen Y and Qi N: Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application. PeerJ. 5:e33012017. View Article : Google Scholar : PubMed/NCBI

15 

Higman MA, Port JD, Beauchamp NJ Jr and Chen AR: Reversible leukoencephalopathy associated with re-infusion of DMSO preserved stem cell. Bone Marrow Transplant. 26:797–800. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Liu Y, Zhou Y, Feng H, Ma GE and Ni Y: Injectable tissue-engineered bone composed of human adipose-derived stromal cell and platelet-rich plasma. Biomaterials. 29:3338–3345. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Shafaei H, Esmaeili A, Mardani M, Razavi S, Hashemibeni B, Nasr-Esfahani MH, Shiran MB and Esfandiari E: Effects of human placental serum on proliferation and morphology of human adipose tissue-derived stem cell. Bone Marrow Transplant. 46:1464–1471. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Jalowiec JM, D'Este M, Bara JJ, Denom J, Menzel U, Alini M, Verrier S and Herrmann M: An in vitro investigation of platelet-rich plasma-gel as a cell and growth factor delivery vehicle for tissue engineering. Tissue Eng Part C Methods. 22:49–58. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Weibrich G, Kleis WK, Hafner G and Hitzler WE: Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg. 30:97–102. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Freymann U, Degrassi L, Kruger JP, Metzlaff S, Endres M and Petersen W: Effect of serum and platelet-rich plasma on human early or advanced degenerative meniscus cells. Connect Tissue Res. 58:509–519. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Bura A, Planat-Benard V, Bourin P, Silvestre JS, Gross F, Grolleau JL, Saint-Lebese B, Peyrafitte JA, Fleury S, Gadelorge M, et al: Phase I trial: The use of autologous cultured adipose-derived stroma/stem cell to treat patients with non-revascularizable critical limb ischemia. Cytotherapy. 16:245–257. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Guo X, Li S, Ji Q, Lian R and Chen J: Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture. Hum Cell. 28:175–189. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Gu H, Guo F, Zhou X, Gong L, Zhang Y, Zhai W, Chen L, Cen L, Yin S, Chang J and Cui L: The stimulation of osteogenic differentiation of human adipose-derived stem cell by ionic products from akermanite dissolution via activation of the ERK pathway. Biomaterials. 32:7023–7033. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Wang J, Ye Y, Tian H, Yang S, Jin X, Tong W and Zhang Y: In vitro osteogenesis of human adipose-derived stem cell by coculture with human umbilical vein endothelial cell. Biochem Biophys Res Commun. 412:143–149. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Visweswaran M, Schiefer L, Arfuso F, Dilley RJ, Newsholme P and Dharmarajan A: Wnt antagonist secreted frizzled-related protein 4 upregulates adipogenic differentiation in human adipose tissue-derived mesenchymal stem cell. PLoS One. 10:e1180052015. View Article : Google Scholar

26 

Li HX, Luo X, Liu RX, Yang YJ and Yang GS: Roles of Wnt/beta-catenin signaling in adipogenic differentiation potential of adipose-derived mesenchymal stem cell. Mol Cell Endocrinol. 291:116–124. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Samuel S, Ahmad RE, Ramasamy TS, Karunanithi P, Naveen SV, Murali MR, Abbas AA and Kamarul T: Platelet-rich concentrate in serum free medium enhances osteogenic differentiation of bone marrow-derived human mesenchymal stromal cell. PeerJ. 4:e23472016. View Article : Google Scholar : PubMed/NCBI

28 

Bunnell BA, Estes BT, Guilak F and Gimble JM: Differentiation of adipose stem cell. Methods Mol Biol. 456:155–171. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Wang X, Spandidos A, Wang H and Seed B: PrimerBank: A PCR primer database for quantitative gene expression analysis, 2012 update. Nucleic Acids Res. 40:D1144–D1149. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Frese L, Dijkman PE and Hoerstrup SP: Adipose tissue-derived stem cells in regenerative medicine. Transfus Med Hemother. 43:268–274. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Martínez-González I, Cruz MJ, Moreno R, Morell F, Muñoz X and Aran JM: Human mesenchymal stem cell resolve airway inflammation, hyperreactivity, and histopathology in a mouse model of occupational asthma. Stem Cells Dev. 23:2352–2363. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Won CH, Yoo HG, Kwon OS, Sung MY, Kang YJ, Chung JH, Park BS, Sung JH, Kim WS and Kim KH: Hair growth promoting effects of adipose tissue-derived stem cell. J Dermatol Sci. 57:134–137. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Hernández-Bule ML, Martínez-Botas J, Trillo MÁ, Paíno CL and Úbeda A: Antiadipogenic effects of subthermal electric stimulation at 448 kHz on differentiating human mesenchymal stem cells. Mol Med Rep. 13:3895–3903. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Rumiński S, Ostrowska B, Jaroszewicz J, Skirecki T, Włodarski K, Święszkowski W and Lewandowska-Szumieł M: Three-dimensional printed polycaprolactone-based scaffolds provide an advantageous environment for osteogenic differentiation of human adipose-derived stem cells. J Tissue Eng Regen Med. 12:e473–e485. 2018. View Article : Google Scholar : PubMed/NCBI

36 

Benazzo F, Botta L, Scaffino MF, Caliogna L, Marullo M, Fusi S and Gastaldi G: Trabecular titanium can induce in vitro osteogenic differentiation of human adipose derived stem cells without osteogenic factors. J Biomed Mater Res A. 102:2061–2071. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Rada T, Reis RL and Gomes ME: Distinct stem cells subpopulations isolated from human adipose tissue exhibit different chondrogenic and osteogenic differentiation potential. Stem Cell Rev. 7:64–76. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Smyth DC, Takenaka S, Yeung C and Richards CD: Oncostatin M regulates osteogenic differentiation of murine adipose-derived mesenchymal progenitor cells through a PKCdelta-dependent mechanism. Cell Tissue Res. 360:309–319. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Regassa A, Suh M, Datar J, Chen C and Kim WK: Fatty acids have different adipogenic differentiation potentials in stromal vascular cells isolated from abdominal fat in laying hens. Lipids. 52:513–522. 2017. View Article : Google Scholar : PubMed/NCBI

40 

Nurden AT, Nurden P, Sanchez M, Andia I and Anitua E: Platelets and wound healing. Front Biosci. 13:3532–3548. 2008.PubMed/NCBI

41 

Whitman DH, Berry RL and Green DM: Platelet gel: An autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 55:1294–1299. 1997. View Article : Google Scholar : PubMed/NCBI

42 

Alsousou J, Thompson M, Hulley P, Noble A and Willett K: The biology of platelet-rich plasma and its application in trauma and orthopaedic surgery: A review of the literature. J Bone Joint Surg Br. 91:987–996. 2009. View Article : Google Scholar : PubMed/NCBI

43 

Nikolidakis D and Jansen JA: The biology of platelet-rich plasma and its application in oral surgery: Literature review. Tissue Eng Part B Rev. 14:249–258. 2008. View Article : Google Scholar : PubMed/NCBI

44 

Anitua E: Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 14:529–535. 1999.PubMed/NCBI

45 

Drago L, Bortolin M, Vassena C, Romanò CL, Taschieri S and Del Fabbro M: Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: An in vitro study. PLoS One. 9:e1078132014. View Article : Google Scholar : PubMed/NCBI

46 

Margolis DJ, Kantor J, Santanna J, Strom BL and Berlin JA: Effectiveness of platelet releasate for the treatment of diabetic neuropathic foot ulcers. Diabetes Care. 24:483–488. 2001. View Article : Google Scholar : PubMed/NCBI

47 

Josh F, Kobe K, Tobita M, Tanaka R, Suzuki K, Ono K, Hyakusoku H and Mizuno H: Accelerated and safe proliferation of human adipose-derived stem cell in medium supplemented with human serum. J Nippon Med Sch. 79:444–452. 2012. View Article : Google Scholar : PubMed/NCBI

48 

Adkins JN, Varnum SM, Auberry KJ, Moore RJ, Angell NH, Smith RD, Springer DL and Pounds JG: Toward a human blood serum proteome: Analysis by multidimensional separation coupled with mass spectrometry. Mol Cell Proteomics. 1:947–955. 2002. View Article : Google Scholar : PubMed/NCBI

49 

Kobayashi T, Watanabe H, Yanagawa T, Tsutsumi S, Kayakabe M, Shinozaki T, Higuchi H and Takagishi K: Motility and growth of human bone-marrow mesenchymal stem cell during ex vivo expansion in autologous serum. J Bone Joint Surg Br. 87:1426–1433. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu YD, Li M, Liao X, Li SH, Yan JX, Fan L, She WL, Song JX and Liu HW: Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use. Mol Med Rep 19: 2189-2201, 2019.
APA
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L. ... Liu, H. (2019). Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use. Molecular Medicine Reports, 19, 2189-2201. https://doi.org/10.3892/mmr.2019.9842
MLA
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L., She, W., Song, J., Liu, H."Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use". Molecular Medicine Reports 19.3 (2019): 2189-2201.
Chicago
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L., She, W., Song, J., Liu, H."Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use". Molecular Medicine Reports 19, no. 3 (2019): 2189-2201. https://doi.org/10.3892/mmr.2019.9842
Copy and paste a formatted citation
x
Spandidos Publications style
Wu YD, Li M, Liao X, Li SH, Yan JX, Fan L, She WL, Song JX and Liu HW: Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use. Mol Med Rep 19: 2189-2201, 2019.
APA
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L. ... Liu, H. (2019). Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use. Molecular Medicine Reports, 19, 2189-2201. https://doi.org/10.3892/mmr.2019.9842
MLA
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L., She, W., Song, J., Liu, H."Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use". Molecular Medicine Reports 19.3 (2019): 2189-2201.
Chicago
Wu, Y., Li, M., Liao, X., Li, S., Yan, J., Fan, L., She, W., Song, J., Liu, H."Effects of storage culture media, temperature and duration on human adipose‑derived stem cell viability for clinical use". Molecular Medicine Reports 19, no. 3 (2019): 2189-2201. https://doi.org/10.3892/mmr.2019.9842
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team