miR‑671‑3p is downregulated in non‑small cell lung cancer and inhibits cancer progression by directly targeting CCND2

Yao,Yuanshan; Zhou,Yinjie; Fu,Xiaojun

doi:10.3892/mmr.2019.9858

miR‑671‑3p is downregulated in non‑small cell lung cancer and inhibits cancer progression by directly targeting CCND2

Authors:
- Yuanshan Yao
- Yinjie Zhou
- Xiaojun Fu
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Affiliations: Department of Thoracic Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 300270, P.R. China, Department of Gastrointestinal Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 300270, P.R. China
Published online on: January 15, 2019 https://doi.org/10.3892/mmr.2019.9858
Pages: 2407-2412

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Abstract

MicroRNAs (miRNAs) are implicated in the development and progression of non‑small cell lung cancer (NSCLC). A previous study suggested that miR‑671‑3p suppresses the development of breast cancer. However, the role of miR‑671‑3p in NSCLC remains largely unknown. In the present study, it was identified that miR‑671‑3p was significantly upregulated in NSCLC tissues compared with adjacent normal tissues by reverse transcription quantitative polymerase chain reaction (RT‑qPCR). Similarly, decreased levels of miR‑671‑3p in NSCLC cell lines were observed compared with those in the non‑tumorigenic human bronchial epithelial NL20 cell line. Cell Counting Kit‑8 and Transwell invasion assays indicated that miR‑671‑3p overexpression suppressed the proliferation and invasion of A549 cells, and vice versa. Mechanistically, it was demonstrated that CCND2 was a direct target of miR‑671‑3p. RT‑qPCR and western blot analysis indicated that miR‑671‑3p overexpression decreased the expression of CCND2 in A549 cells. Furthermore, rescue experiments demonstrated that the restoration of CCND2 may significantly reverse the suppressive roles of miR‑671‑3p overexpression on NSCLC cell proliferation and invasion. Taken together, the present study demonstrated that miR‑671‑3p exerted its tumor‑suppressive roles via directly targeting CCND2 in NSCLC.

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1	Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI
2	Ettinger DS: Ten years of progress in non-small cell lung cancer. J Natl Compr Canc Netw. 10:292–295. 2012. View Article : Google Scholar : PubMed/NCBI
3	Xiao F, Liu D, Guo Y, Shi B, Song Z, Tian Y, Zhang Z and Liang C: Survival rate and prognostic factors of surgically resected clinically synchronous multiple primary non-small cell lung cancer and further differentiation from intrapulmonary metastasis. J Thorac Dis. 9:990–1001. 2017. View Article : Google Scholar : PubMed/NCBI
4	Chen X, Du J, Jiang R and Li L: MicroRNA-214 inhibits the proliferation and invasion of lung carcinoma cells by targeting JAK1. Am J Transl Res. 10:1164–1171. 2018.PubMed/NCBI
5	Saumet A and Lecellier CH: microRNAs and personalized medicine: Evaluating their potential as cancer biomarkers. Adv Exp Med Biol. 888:5–15. 2015. View Article : Google Scholar : PubMed/NCBI
6	Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI
7	Garzon R, Calin GA and Croce CM: MicroRNAs in cancer. Annu Rev Med. 60:167–179. 2009. View Article : Google Scholar : PubMed/NCBI
8	Ren P, Gong F, Zhang Y, Jiang J and Zhang H: MicroRNA-92a promotes growth, metastasis and chemoresistance in non-small cell lung cancer cells by targeting PTEN. Tumour Biol. 37:3215–3225. 2016. View Article : Google Scholar : PubMed/NCBI
9	Lv X, Li CY, Han P and Xu XY: MicroRNA-520a-3p inhibits cell growth and metastasis of non-small cell lung cancer through PI3K/AKT/mTOR signaling pathway. Eur Rev Med Pharmacol Sci. 22:2321–2327. 2018.PubMed/NCBI
10	Li S, Zhang J, Zhao Y, Wang F, Chen Y and Fei X: miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells. Oncol Lett. 15:7069–7075. 2018.PubMed/NCBI
11	Xiong DD, Chen H, He RQ, Lan AH, Zhong JC, Chen G, Feng ZB and Wei KL: MicroRNA-671-3p inhibits the development of breast cancer: A study based on in vitro experiments, in-house quantitative polymerase chain reaction and bioinformatics analysis. Int J Oncol. 52:1801–1814. 2018.PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Jiang B, Li M, Ji F and Nie Y: MicroRNA-219 exerts a tumor suppressive role in glioma via targeting Sal-like protein 4. Exp Ther Med. 14:6213–6221. 2017.PubMed/NCBI
14	Goldstraw P, Crowley J, Chansky K, Giroux DJ, Groome PA, Rami-Porta R, Postmus PE, Rusch V and Sobin L; International Association for the Study of Lung Cancer International Staging Committee; Participating Institutions, : The IASLC lung cancer staging project: Proposals for the revision of the TNM stage groupings in the forthcoming (seventh) edition of the TNM classification of malignant tumours. J Thorac Oncol. 2:706–714. 2007. View Article : Google Scholar : PubMed/NCBI
15	Onat S, Ates G, Avci A, Yildiz T, Birak A, Akgul Ozmen C and Ulku R: The role of mediastinoscopy in the diagnosis of non-lung cancer diseases. Ther Clin Risk Manag. 13:939–943. 2017. View Article : Google Scholar : PubMed/NCBI
16	Yu YX, Wang Y and Liu H: Overexpression of PTEN suppresses non-small-cell lung carcinoma metastasis through inhibition of integrin αVβ6 signaling. Am J Transl Res. 9:3304–3314. 2017.PubMed/NCBI
17	Wang S, Zhang B, Li C, Cui C, Yue D, Shi B, Zhang Q, Zhang Z, Zhang X and Wang C: Prognostic value of number of negative lymph node in patients with stage II and IIIa non-small cell lung cancer. Oncotarget. 8:79387–79396. 2017.PubMed/NCBI
18	Esmatabadi MJD, Farhangi B, Montazeri M, Monfared H, Sistani RN and Sadeghizadeh M: Up-regulation of miR-21 decreases chemotherapeutic effect of dendrosomal curcumin in breast cancer cells. Iran J Basic Med Sci. 20:350–359. 2017.PubMed/NCBI
19	Du L, Chen T, Zhao K and Yang D: miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression. Onco Targets Ther. 11:2195–2202. 2018. View Article : Google Scholar : PubMed/NCBI
20	Jeon SH, Yoo JK, Kim CM, Lim ES, Lee SJ, Lee JM, Oh SH and Kim JK: The novel hsa-miR-12528 regulates tumourigenesis and metastasis through hypo-phosphorylation of AKT cascade by targeting IGF-1R in human lung cancer. Cell Death Dis. 9:4932018. View Article : Google Scholar : PubMed/NCBI
21	Xu J, Pan X and Hu Z: MiR-502 mediates esophageal cancer cell TE1 proliferation by promoting AKT phosphorylation. Biochem Biophys Res Commun. 501:119–123. 2018. View Article : Google Scholar : PubMed/NCBI
22	Yu Y, Wang Z, Sun D, Zhou X, Wei X, Hou W, Ding Y, Ma Y and Hou Y: miR-671 promotes prostate cancer cell proliferation by targeting tumor suppressor SOX6. Eur J Pharmacol. 823:65–71. 2018. View Article : Google Scholar : PubMed/NCBI
23	Yang F, Wei K, Qin Z, Liu W, Shao C, Wang C, Ma L, Xie M, Shu Y and Shen H: MiR-598 suppresses invasion and migration by negative regulation of derlin-1 and epithelial-mesenchymal transition in non-small cell lung cancer. Cell Physiol Biochem. 47:245–256. 2018. View Article : Google Scholar : PubMed/NCBI
24	Li YL, Wang J, Zhang CY, Shen YQ, Wang HM, Ding L, Gu YC, Lou JT, Zhao XT, Ma ZL and Jin YX: MiR-146a-5p inhibits cell proliferation and cell cycle progression in NSCLC cell lines by targeting CCND1 and CCND2. Oncotarget. 7:59287–59298. 2016.PubMed/NCBI
25	Wang Y, Xue J, Kuang H, Zhou X, Liao L and Yin F: microRNA-1297 inhibits the growth and metastasis of colorectal cancer by suppressing cyclin D2 expression. DNA Cell Biol. 36:991–999. 2017. View Article : Google Scholar : PubMed/NCBI