Open Access

Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays

  • Authors:
    • Zhenjie Sun
    • Yuhui Sun
    • Yumeng Li
    • Xiuli Luan
    • Hui Chen
    • Haiying Wu
    • Bo Peng
    • Chunxue Lu
  • View Affiliations

  • Published online on: January 16, 2020     https://doi.org/10.3892/mmr.2020.10947
  • Pages: 1572-1580
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. In order to improve understanding of the roles of GlgA in chlamydial pathogenesis, four proteins that interact with GlgA, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin‑like actin‑capping protein and apolipoprotein A‑I binding protein were identified using yeast two‑hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of GlgA in C. trachomatis infection.
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March 2020
Volume 21 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Sun, Z., Sun, Y., Li, Y., Luan, X., Chen, H., Wu, H. ... Lu, C. (2020). Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays. Molecular Medicine Reports, 21, 1572-1580. https://doi.org/10.3892/mmr.2020.10947
MLA
Sun, Z., Sun, Y., Li, Y., Luan, X., Chen, H., Wu, H., Peng, B., Lu, C."Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays". Molecular Medicine Reports 21.3 (2020): 1572-1580.
Chicago
Sun, Z., Sun, Y., Li, Y., Luan, X., Chen, H., Wu, H., Peng, B., Lu, C."Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays". Molecular Medicine Reports 21, no. 3 (2020): 1572-1580. https://doi.org/10.3892/mmr.2020.10947