Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays

Sun,Zhenjie; Sun,Yuhui; Li,Yumeng; Luan,Xiuli; Chen,Hui; Wu,Haiying; Peng,Bo; Lu,Chunxue

doi:10.3892/mmr.2020.10947

Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays

Authors:
- Zhenjie Sun
- Yuhui Sun
- Yumeng Li
- Xiuli Luan
- Hui Chen
- Haiying Wu
- Bo Peng
- Chunxue Lu
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Affiliations: Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Pathology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China
Published online on: January 16, 2020 https://doi.org/10.3892/mmr.2020.10947
Pages: 1572-1580
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. In order to improve understanding of the roles of GlgA in chlamydial pathogenesis, four proteins that interact with GlgA, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin‑like actin‑capping protein and apolipoprotein A‑I binding protein were identified using yeast two‑hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of GlgA in C. trachomatis infection.

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