Open Access

Icariin promotes osteogenic differentiation of BMSCs by upregulating BMAL1 expression via BMP signaling

  • Authors:
    • Zengfa Huang
    • Hui Wei
    • Xiang Wang
    • Jianwei Xiao
    • Zuoqin Li
    • Yuanliang Xie
    • Yun Hu
    • Xiang Li
    • Zheng Wang
    • Shutong Zhang
  • View Affiliations

  • Published online on: January 20, 2020     https://doi.org/10.3892/mmr.2020.10954
  • Pages: 1590-1596
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing research has demonstrated that expression of brain and muscle ARNT‑like 1 (BMAL1) and other circadian clock genes can be regulated by drugs and toxicants. We previously demonstrated that icariin, extracted from Herba Epimedii, sromotes osteogenic differentiation. However, the mechanism underlying the association between icariin and BMAL1 in osteogenic differentiation of bone marrow‑derived mesenchymal stem cells (BMSCs) remains unclear. The present study was designed with an aim to clarify the association between icariin and BMAL1 in osteogenic differentiation of BMSCs. The Cell Counting Kit‑8 assay was used to evaluate cell proliferation. The expression of bone morphogenetic protein 2 (BMP2), RUNX family transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteocalcin (OC) and BMAL1 in BMSCs was evaluated by reverse transcription‑quantitative PCR and western blotting. ALP and Alizarin red S (ARS) staining were also performed. Icariin promoted BMSC proliferation, and upregulated expression of osteogenic genes and BMAL1. In addition, expression of the osteogenic genes BMP2, RUNX2, ALP and OC were upregulated by BMAL1 overexpression. Furthermore, we confirmed that BMAL1 deficiency suppressed osteogenic differentiation in BMSCs. Finally, ARS staining of BMAL1‑/‑ BMSCs revealed that BMAL1 was an essential intermediary in matrix mineralization during osteogenic differentiation. In conclusion, these results demonstrated that icariin promoted osteogenic differentiation through BMAL1‑BMP2 signaling in BMSCs. The present study thus described a novel target of icariin that has potential applications in the treatment of osteogenic disorders.
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March 2020
Volume 21 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Huang, Z., Wei, H., Wang, X., Xiao, J., Li, Z., Xie, Y. ... Zhang, S. (2020). Icariin promotes osteogenic differentiation of BMSCs by upregulating BMAL1 expression via BMP signaling. Molecular Medicine Reports, 21, 1590-1596. https://doi.org/10.3892/mmr.2020.10954
MLA
Huang, Z., Wei, H., Wang, X., Xiao, J., Li, Z., Xie, Y., Hu, Y., Li, X., Wang, Z., Zhang, S."Icariin promotes osteogenic differentiation of BMSCs by upregulating BMAL1 expression via BMP signaling". Molecular Medicine Reports 21.3 (2020): 1590-1596.
Chicago
Huang, Z., Wei, H., Wang, X., Xiao, J., Li, Z., Xie, Y., Hu, Y., Li, X., Wang, Z., Zhang, S."Icariin promotes osteogenic differentiation of BMSCs by upregulating BMAL1 expression via BMP signaling". Molecular Medicine Reports 21, no. 3 (2020): 1590-1596. https://doi.org/10.3892/mmr.2020.10954