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Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model

  • Authors:
    • Mario S. Oliveira
    • Leonardo Y. Tanaka
    • Ednei L. Antonio
    • Laura I. Brandizzi
    • Andrey J. Serra
    • Leonardo Dos Santos
    • José E. Krieger
    • Francisco R.M. Laurindo
    • Paulo J.F. Tucci
  • View Affiliations / Copyright

    Affiliations: Division of Cardiology, Federal University of São Paulo (UNIFESP), São Paulo 04039‑032, Brazil, Vascular Biology Laboratory, Heart Institute, University of São Paulo (USP), São Paulo 05403‑900, Brazil, Department of Physiological Sciences, Federal University of Espírito Santo (UFES), Vitória, Espírito Santo 29043‑215, Brazil, Laboratory of Genetics and Molecular Cardiology, Heart Institute, University of São Paulo (USP), São Paulo 05403‑900, Brazil
    Copyright: © Oliveira et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1431-1438
    |
    Published online on: January 29, 2020
       https://doi.org/10.3892/mmr.2020.10968
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Abstract

Among the mechanisms of action of hyperbaric oxygenation (HBO), the chance of reducing injury by interfering with the mechanisms of redox homeostasis in the heart leads to the possibility of extending the period of viability of the myocardium at risk. This would benefit late interventions for reperfusion to the ischemic area. The objective of the present study was to investigate the changes in the redox system associated with HBO therapy maintained during the first hour after coronary occlusion in an acute myocardial infarction (MI) rat model. Surviving male rats (n=105) were randomly assigned to one of three groups: Sham (SH=26), myocardial infarction (MI=45) and infarction+hyperbaric therapy (HBO=34, 1 h at 2.5 atm). After 90 min of coronary occlusion, a sample of the heart was collected for western blot analysis of total protein levels of superoxide dismutase, catalase, peroxiredoxin and 3‑nitrotyrosine. Glutathione was measured by enzyme‑linked immunosorbent assay (ELISA). The detection of the superoxide radical anion was carried out by oxidation of dihydroethidium analyzed with confocal microscopy. The mortality rate of the MI group was significantly higher than that of the HBO group. No difference was noted in the myocardial infarction size. The oxidized/reduced glutathione ratio and peroxiredoxin were significantly higher in the SH and MI when compared to the HBO group. Superoxide dismutase enzymes and catalase were significantly higher in the HBO group compared to the MI and SH groups. 3‑Nitrotyrosine and the superoxide radical were significantly lower in the HBO group compared to these in the MI and SH groups. These data demonstrated that hyperbaric oxygenation therapy decreased mortality by improving redox control in the hearts of rats in the acute phase of myocardial infarction.
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Copy and paste a formatted citation
Spandidos Publications style
Oliveira MS, Tanaka LY, Antonio EL, Brandizzi LI, Serra AJ, Dos Santos L, Krieger JE, Laurindo FR and Tucci PJ: Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model. Mol Med Rep 21: 1431-1438, 2020.
APA
Oliveira, M.S., Tanaka, L.Y., Antonio, E.L., Brandizzi, L.I., Serra, A.J., Dos Santos, L. ... Tucci, P.J. (2020). Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model. Molecular Medicine Reports, 21, 1431-1438. https://doi.org/10.3892/mmr.2020.10968
MLA
Oliveira, M. S., Tanaka, L. Y., Antonio, E. L., Brandizzi, L. I., Serra, A. J., Dos Santos, L., Krieger, J. E., Laurindo, F. R., Tucci, P. J."Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model". Molecular Medicine Reports 21.3 (2020): 1431-1438.
Chicago
Oliveira, M. S., Tanaka, L. Y., Antonio, E. L., Brandizzi, L. I., Serra, A. J., Dos Santos, L., Krieger, J. E., Laurindo, F. R., Tucci, P. J."Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model". Molecular Medicine Reports 21, no. 3 (2020): 1431-1438. https://doi.org/10.3892/mmr.2020.10968
Copy and paste a formatted citation
x
Spandidos Publications style
Oliveira MS, Tanaka LY, Antonio EL, Brandizzi LI, Serra AJ, Dos Santos L, Krieger JE, Laurindo FR and Tucci PJ: Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model. Mol Med Rep 21: 1431-1438, 2020.
APA
Oliveira, M.S., Tanaka, L.Y., Antonio, E.L., Brandizzi, L.I., Serra, A.J., Dos Santos, L. ... Tucci, P.J. (2020). Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model. Molecular Medicine Reports, 21, 1431-1438. https://doi.org/10.3892/mmr.2020.10968
MLA
Oliveira, M. S., Tanaka, L. Y., Antonio, E. L., Brandizzi, L. I., Serra, A. J., Dos Santos, L., Krieger, J. E., Laurindo, F. R., Tucci, P. J."Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model". Molecular Medicine Reports 21.3 (2020): 1431-1438.
Chicago
Oliveira, M. S., Tanaka, L. Y., Antonio, E. L., Brandizzi, L. I., Serra, A. J., Dos Santos, L., Krieger, J. E., Laurindo, F. R., Tucci, P. J."Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model". Molecular Medicine Reports 21, no. 3 (2020): 1431-1438. https://doi.org/10.3892/mmr.2020.10968
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