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Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis

  • Authors:
    • Danye Li
    • Caiyan Gao
    • Ling Zhao
    • Yongming Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pulmonary and Critical Care Medicine, China‑Japan Friendship Hospital, Beijing 100029, P.R. China, Department of Respiration, Hongda Hospital of Jiamusi University, Jiamusi, Heilongjiang 154004, P.R. China, Department of Pathology, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2513-2521
    |
    Published online on: April 2, 2020
       https://doi.org/10.3892/mmr.2020.11053
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Abstract

Long intergenic non-coding RNAs (lincRNAs) are long non‑coding transcripts from the intergenic regions of annotated protein‑coding genes. lincRNA cyclooxygenase 2 (Cox2) is an early‑primary response gene regulated by the NF‑κB signaling pathway in macrophages. It was found that lincRNACox2 was significantly increased in patients with the Mycobacterium tuberculosis (M. tuberculosis) H37Ra strain infection and macrophages, using reverse transcription-quantitative PCR (RT‑qPCR). ELISA, western blotting and RT‑qPCR results indicated that the inflammatory response factors tumor necrosis factor‑α, interferon‑γ, interleukin‑6, Cox2 and inducible nitric oxide synthase were significantly increased in H37Ra infected macrophages. In addition, the inflammatory regulating proteins NF‑κB and Stat3 were significantly increased in H37Ra infected macrophages but decreased in lincRNACox2 knockdown macrophages infected with H37Ra. Moreover, the knockdown of lincRNACox2 increased the apoptotic rate of H37Ra infected macrophages and facilitated the proliferation of H37Ra. Collectively, the present results suggested that lincRNACox2 may be required for the activation of NF‑κB and Stat3, in order to regulate inflammatory responses involved in resistance to M. tuberculosis infection.
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Copy and paste a formatted citation
Spandidos Publications style
Li D, Gao C, Zhao L and Zhang Y: Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis. Mol Med Rep 21: 2513-2521, 2020.
APA
Li, D., Gao, C., Zhao, L., & Zhang, Y. (2020). Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis. Molecular Medicine Reports, 21, 2513-2521. https://doi.org/10.3892/mmr.2020.11053
MLA
Li, D., Gao, C., Zhao, L., Zhang, Y."Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis". Molecular Medicine Reports 21.6 (2020): 2513-2521.
Chicago
Li, D., Gao, C., Zhao, L., Zhang, Y."Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis". Molecular Medicine Reports 21, no. 6 (2020): 2513-2521. https://doi.org/10.3892/mmr.2020.11053
Copy and paste a formatted citation
x
Spandidos Publications style
Li D, Gao C, Zhao L and Zhang Y: Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis. Mol Med Rep 21: 2513-2521, 2020.
APA
Li, D., Gao, C., Zhao, L., & Zhang, Y. (2020). Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis. Molecular Medicine Reports, 21, 2513-2521. https://doi.org/10.3892/mmr.2020.11053
MLA
Li, D., Gao, C., Zhao, L., Zhang, Y."Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis". Molecular Medicine Reports 21.6 (2020): 2513-2521.
Chicago
Li, D., Gao, C., Zhao, L., Zhang, Y."Inflammatory response is modulated by lincRNACox2 via the NF‑κB pathway in macrophages infected by Mycobacterium tuberculosis". Molecular Medicine Reports 21, no. 6 (2020): 2513-2521. https://doi.org/10.3892/mmr.2020.11053
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