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Article Open Access

MEG3 regulates apoptosis of adipose‑derived stem cells

  • Authors:
    • Yao Shi
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2435-2442
    |
    Published online on: April 8, 2020
       https://doi.org/10.3892/mmr.2020.11059
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Abstract

In plastic surgery, the maneuverability and safety of autologous fat transplantation have become increasingly recognized and continuously improved. However, the uncertainty of adipocyte survival makes it difficult to predict postoperative effects. Adipose‑derived stem cells (ADSCs) exhibit remarkable paracrine activity, and the number of ADSCs in adipose tissue is closely related to tissue survival. Maternally expressed gene 3 (MEG3) is known to modulate the apoptosis of various cell types. The present study aimed to evaluate the hypothesis that MEG3 serves an important role in ADSC apoptosis by regulating the expression of p53, and to explore the regulatory mechanisms of p53 in ADSC apoptosis. MEG3 was overexpressed in ADSCs and these cells were evaluated for viability, TP53 expression, apoptosis, morphology, and Bax and Bcl‑2 expression by performing MTT assays, reverse transcription‑quantitative PCR, flow cytometry analysis and western blotting. This study demonstrated that MEG3 may have an important role in the spontaneous apoptosis of ADSCs, and apoptosis induced by oxidative stress. In addition, this study revealed that p53 had a regulatory role in the downstream Bcl‑2/Bax pathway. This study provides insight into the role of MEG3 in ADSC apoptosis, thereby facilitating the survival of ADSCs during adipose tissue transplantation. Further in vivo and in vitro experiments should be conducted, along with the development of clinical applications.
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1 

Rohrich RJ: The American Society of Plastic Surgeons' procedural statistics: What they really mean. Plast Reconstr Surg. 112:1389–1392. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Claro F Jr, Figueiredo JC, Zampar AG and Pinto-Neto AM: Applicability and safety of autologous fat for reconstruction of the breast. Br J Surg. 99:768–780. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Wang CF, Zhou Z, Yan YJ, Zhao DM, Chen F and Qiao Q: Clinical analyses of clustered microcalcifications after autologous fat injection for breast augmentation. Plast Reconstr Surg. 127:1669–1673. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Coleman SR: Structural fat grafting: More than a permanent filler. Plast Reconstr Surg. 118 (3 Suppl):S108–S120. 2006. View Article : Google Scholar

5 

Lu F, Li J, Gao J, Ogawa R, Ou C, Yang B and Fu B: Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg. 124:1437–1446. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Kirkham JC, Lee JH, Medina MA III, McCormack MC, Randolph MA and Austen WG Jr: The impact of liposuction cannula size on adipocyte viability. Ann Plast Surg. 69:479–481. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Nguyen A, Pasyk KA, Bouvier TN, Hassett CA and Argenta LC: Comparative study of survival of autologous adipose tissue taken and transplanted by different techniques. Plast Reconstr Surg. 85:378–389. 1990. View Article : Google Scholar : PubMed/NCBI

8 

Billings E Jr and May JW Jr: Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg. 83:368–381. 1989. View Article : Google Scholar : PubMed/NCBI

9 

Pal R, Venkataramana NK, Bansal A, Balaraju S, Jan M, Chandra R, Dixit A, Rauthan A, Murgod U and Totey S: Ex vivo-expanded autologous bone marrow-derived mesenchymal stromal cells in human spinal cord injury/paraplegia: A pilot clinical study. Cytotherapy. 11:897–911. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Zuk PA: The adipose-derived stem cell: Looking back and looking ahead. Mol Biol Cell. 21:1783–1787. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Ionescu L, Byrne RN, van Haaften T, Vadivel A, Alphonse RS, Rey-Parra GJ, Weissmann G, Hall A, Eaton F and Thébaud B: Stem cell conditioned medium improves acute lung injury in mice: In vivo evidence for stem cell paracrine action. Am J Physiol Lung Cell Mol Physiol. 303:L967–L977. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Fouraschen SM, Pan Q, de Ruiter PE, Farid WR, Kazemier G, Kwekkeboom J, Ijzermans JN, Metselaar HJ, Tilanus HW, de Jonge J and van der Laan LJ: Secreted factors of human liver-derived mesenchymal stem cells promote liver regeneration early after partial hepatectomy. Stem Cells Dev. 21:2410–2419. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP and Hedrick MH: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Kern S, Eichler H, Stoeve J, Klüter H and Bieback K: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 24:1294–1301. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Su M, Guan H, Zhang F, Gao Y, Teng X and Yang W: HDAC6 regulates the chaperone-mediated autophagy to prevent oxidative damage in injured neurons after experimental spinal cord injury. Oxid Med Cell Longev. 2016:72637362016. View Article : Google Scholar : PubMed/NCBI

16 

Chen X, Yan L, Guo Z, Chen Z, Chen Y, Li M, Huang C, Zhang X and Chen L: Adipose-derived mesenchymal stem cells promote the survival of fat grafts via crosstalk between the Nrf2 and TLR4 pathways. Cell Death Dis. 7:e23692016. View Article : Google Scholar : PubMed/NCBI

17 

Zhang X, Rice K, Wang Y, Chen W, Zhong Y, Nakayama Y, Zhou Y and Klibanski A: Maternally expressed gene 3 (MEG3) noncoding ribonucleic acid: Isoform structure, expression, and functions. Endocrinology. 151:939–947. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Luo G, Wang M, Wu X, Tao D, Xiao X, Wang L, Min F, Zeng F and Jiang G: Long non-coding RNA MEG3 inhibits cell proliferation and induces apoptosis in prostate cancer. Cell Physiol Biochem. 37:2209–2220. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Peng W, Si S, Zhang Q, Li C, Zhao F, Wang F, Yu J and Ma R: Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression. J Exp Clin Cancer Res. 34:792015. View Article : Google Scholar : PubMed/NCBI

20 

Zhang X, Zhou Y, Mehta KR, Danila DC, Scolavino S, Johnson SR and Klibanski A: A pituitary-derived MEG3 isoform functions as a growth suppressor in tumor cells. J Clin Endocrinol Metab. 88:5119–5126. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Lu KH, Li W, Liu XH, Sun M, Zhang ML, Wu WQ, Xie WP and Hou YY: Long non-coding RNA MEG3 inhibits NSCLC cells proliferation and induces apoptosis by affecting p53 expression. BMC Cancer. 13:4612013. View Article : Google Scholar : PubMed/NCBI

22 

Zhou Y, Zhong Y, Wang Y, Zhang X, Batista DL, Gejman R, Ansell PJ, Zhao J, Weng C and Klibanski A: Activation of p53 by MEG3 non-coding RNA. J Biol Chem. 282:24731–24742. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Neuber F: Fettransplantation. Chir Kongr Verhandl Dsch Gesellch Chir. 22:661893.

25 

Coleman SR: Hand rejuvenation with structural fat grafting. Plast Reconstr Surg. 110:1731–1747. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, Sato T, Aiba-Kojima E, Iizuka F, Inoue K, Suga H and Yoshimura K: Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 12:3375–3382. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Moseley TA, Zhu M and Hedrick MH: Adipose-derived stem and progenitor cells as fillers in plastic and reconstructive surgery. Plast Reconstr Surg. 118 (3 Suppl):S121–S128. 2006. View Article : Google Scholar

28 

Toyserkani NM, Quaade ML and Sørensen JA: Cell-Assisted Lipotransfer: A systematic review of its efficacy. Aesthetic Plast Surg. 40:309–318. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Ponting CP, Oliver PL and Reik W: Evolution and functions of long noncoding RNAs. Cell. 136:629–641. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Ying L, Huang Y, Chen H, Wang Y, Xia L, Chen Y, Liu Y and Qiu F: Downregulated MEG3 activates autophagy and increases cell proliferation in bladder cancer. Mol Biosyst. 9:407–411. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Sun M, Xia R, Jin F, Xu T, Liu Z, De W and Liu X: Downregulated long noncoding RNA MEG3 is associated with poor prognosis and promotes cell proliferation in gastric cancer. Tumour Biol. 35:1065–1073. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Shi Y, Lv C, Shi L and Tu G: MEG3 inhibits proliferation and invasion and promotes apoptosis of human osteosarcoma cells. Oncol Lett. 15:1917–1923. 2018.PubMed/NCBI

33 

Carrière A, Ebrahimian TG, Dehez S, Augé N, Joffre C, André M, Arnal S, Duriez M, Barreau C, Arnaud E, et al: Preconditioning by mitochondrial reactive oxygen species improves the proangiogenic potential of adipose-derived cells-based therapy. Arterioscler Thromb Vasc Biol. 29:1093–1099. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi Y: MEG3 regulates apoptosis of adipose‑derived stem cells. Mol Med Rep 21: 2435-2442, 2020.
APA
Shi, Y. (2020). MEG3 regulates apoptosis of adipose‑derived stem cells. Molecular Medicine Reports, 21, 2435-2442. https://doi.org/10.3892/mmr.2020.11059
MLA
Shi, Y."MEG3 regulates apoptosis of adipose‑derived stem cells". Molecular Medicine Reports 21.6 (2020): 2435-2442.
Chicago
Shi, Y."MEG3 regulates apoptosis of adipose‑derived stem cells". Molecular Medicine Reports 21, no. 6 (2020): 2435-2442. https://doi.org/10.3892/mmr.2020.11059
Copy and paste a formatted citation
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Spandidos Publications style
Shi Y: MEG3 regulates apoptosis of adipose‑derived stem cells. Mol Med Rep 21: 2435-2442, 2020.
APA
Shi, Y. (2020). MEG3 regulates apoptosis of adipose‑derived stem cells. Molecular Medicine Reports, 21, 2435-2442. https://doi.org/10.3892/mmr.2020.11059
MLA
Shi, Y."MEG3 regulates apoptosis of adipose‑derived stem cells". Molecular Medicine Reports 21.6 (2020): 2435-2442.
Chicago
Shi, Y."MEG3 regulates apoptosis of adipose‑derived stem cells". Molecular Medicine Reports 21, no. 6 (2020): 2435-2442. https://doi.org/10.3892/mmr.2020.11059
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