Open Access

Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma

  • Authors:
    • Jie Yang
    • Ying Li
    • Zuochun Yu
    • Yuefen Zhou
    • Jianfei Tu
    • Jian Lou
    • Yonghui Wang
  • View Affiliations

  • Published online on: April 10, 2020     https://doi.org/10.3892/mmr.2020.11069
  • Pages: 2395-2404
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) has become a major cause of cancer‑related mortality worldwide. Circular RNAs (circRNAs) are non‑coding RNAs that serve important roles in multiple cancers. However, the role of circRNAs in HCC remains largely unknown. In the present study, a circRNA microarray dataset of HCC samples, GSE97332, was downloaded from the gene expression omnibus database. Following data preprocessing, differentially expressed circRNAs between HCC tissues and normal tissues were determined using GEO2R. The circRNA‑miRNA interactions were predicted by the miRanda database. The miRTarbase database was used to search for target genes of the miRNAs. A circRNA‑miRNA‑mRNA network was constructed using Cytoscape based on the obtained circRNA, miRNA and mRNA. In this network, the upregulated circRNA hsa_circRNA_100084 was found to be involved in a competing endogenous relationship of hsa_circRNA_100084‑hsa‑miR‑23a‑5p‑ insulin‑like growth factor 2 (IGF2). The differential expression of hsa_circRNA_100084, hsa‑miR‑23a‑5p and IGF2 in HCC tissues and liver cancer cells was validated by reverse transcription‑quantitative PCR. Additionally, the interactions between hsa‑miR‑23a‑5p with hsa_circRNA_100084 and IGF2 were validated by dual‑luciferase reporter assays. Knocking down hsa_circRNA_100084 inhibited the proliferation, migration and invasion of liver cancer cells, while the simultaneous overexpression of IGF2 reversed the effects of hsa_circRNA_100084 knockdown. The results show that hsa_circRNA_100084 could promote the expression of IGF2 by acting as a sponge of hsa‑miR‑23a‑5p in liver cancer cells.
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June-2020
Volume 21 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yang J, Li Y, Yu Z, Zhou Y, Tu J, Lou J and Wang Y: Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma. Mol Med Rep 21: 2395-2404, 2020
APA
Yang, J., Li, Y., Yu, Z., Zhou, Y., Tu, J., Lou, J., & Wang, Y. (2020). Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma. Molecular Medicine Reports, 21, 2395-2404. https://doi.org/10.3892/mmr.2020.11069
MLA
Yang, J., Li, Y., Yu, Z., Zhou, Y., Tu, J., Lou, J., Wang, Y."Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma". Molecular Medicine Reports 21.6 (2020): 2395-2404.
Chicago
Yang, J., Li, Y., Yu, Z., Zhou, Y., Tu, J., Lou, J., Wang, Y."Circular RNA Circ100084 functions as sponge of miR‑23a‑5p to regulate IGF2 expression in hepatocellular carcinoma". Molecular Medicine Reports 21, no. 6 (2020): 2395-2404. https://doi.org/10.3892/mmr.2020.11069