Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
July-2020 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2020 Volume 22 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells

  • Authors:
    • Yingjie Zhang
    • Lishu Zhou
    • Zhaoqiang Zhang
    • Fei Ren
    • Liangjiao Chen
    • Zedong Lan
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Oral Medicine, Stomatological Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Orthodontics, Stomatological Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China, Department of Orthodontics, Shenzhen Stomatological Hospital, Southern Medical University, Shenzhen, Guangdong 518001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 135-144
    |
    Published online on: May 4, 2020
       https://doi.org/10.3892/mmr.2020.11110
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The use of human bone marrow mesenchymal stem cells (hBMSCs) as a tissue engineering application for individuals affected by osteoporosis and other types of bone loss diseases has been well studied in recent years. The osteogenic differentiation of hBMSCs can be regulated by a number of cues. MicroRNAs (miRNAs/miRs) serve as the key regulators of various biological processes; however, to the best of our knowledge, no information exists with regards to the specific modulatory effects of miR‑10a‑5p on osteogenic differentiation of hBMSCs. The aim of the present study was to investigate the relationship between hBMSCs and miR‑10a‑5p and, ultimately, to determine how miR‑10a‑5p affects the osteogenic differentiation process of hBMSCs in vitro and in vivo. The hBMSCs used in the present study were transfected with mirVana™ miRNA inhibitors and mimics, and transfection efficiency was assessed by fluorescence microscopy and reverse transcription‑quantitative PCR (RT‑qPCR). Viability of hBMSCs following transfection was analyzed using a Cell Counting Kit‑8 assay. The mRNA expression levels of specific osteoblast markers, including alkaline phosphatase (ALP) and runt‑related transcription factor 2 (RUNX2) were measured using RT‑qPCR and western blot analysis. New bone formation was evaluated by Goldner's trichrome staining and micro‑CT analysis in vivo. No significant difference in cell viability was observed among the different groups 24 h post‑transfection. Overexpression of miR‑10a‑5p inhibited the expression of osteoblast makers in hBMSCs, whereas inhibition of miR‑10a‑5p upregulated the expression of ALP and RUNX2 in vitro. Furthermore, miR‑10a‑5p acted as a suppressor during the process of new bone formation in vivo. In conclusion, the findings of the present study suggested that miR‑10a‑5p served as a negative regulatory factor during osteoblast differentiation of hBMSCs and may be utilized in a treatment approach for bone repair in osteogenic‑related diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Pichler K, Loreto C, Leonardi R, Reuber T, Weinberg AM and Musumeci G: Rankl is downregulated in bone cells by physical activity (treadmill and vibration stimulation training) in rat with glucocorticoid-induced osteoporosis. Histol Histopathol. 28:1185–1196. 2013.PubMed/NCBI

2 

Castrogiovanni P, Trovato FM, Szychlinska MA, Nsir H, Imbesi R and Musumeci G: The importance of physical activity in osteoporosis. From the molecular pathways to the clinical evidence. Histol Histopathol. 31:1183–1194. 2016.PubMed/NCBI

3 

Cardinale M and Bosco C: The use of vibration as an exercise intervention. Exerc Sport Sci Rev. 31:3–7. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Srinivasaiah S, Musumeci G, Mohan T, Castrogiovanni P, Absenger-Novak M, Zefferer U, Mostofi S, Bonyadi Rad E, Grün NG, Weinberg AM and Schäfer U: A 300 µm organotypic bone slice culture model for temporal investigation of endochondral osteogenesis. Tissue Eng Part C Methods. 25:197–212. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Szychlinska MA, Castrogiovanni P, Nsir H, Di Rosa MD, Guglielmino C, Parenti R, Calabrese G, Pricoco E, Salvatorelli L, Magro G, et al: Engineered cartilage regeneration from adipose tissue derived-mesenchymal stem cells: A morphomolecular study on osteoblast, chondrocyte and apoptosis evaluation. Exp Cell Res. 357:222–235. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Yamada Y, Ueda M, Hibi H and Nagasaka T: Translational research for injectable tissue-engineered bone regeneration using mesenchymal stem cells and platelet-rich plasma: From basic research to clinical case study. Cell Transplant. 13:343–355. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Yamada Y, Nakamura S, Ito K, Kohgo T, Hibi H, Nagasaka T and Ueda M: Injectable tissue-engineered bone using autogenous bone marrow-derived stromal cells for maxillary sinus augmentation: Clinical application report from a 2-6-year follow-up. Tissue Eng Part A. 14:1699–1707. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Lau NC, Lim LP, Weinstein EG and Bartel DP: An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science. 294:858–862. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Rana TM: Illuminating the silence: Understanding the structure and function of small RNAs. Nat Rev Mol Cell Biol. 8:23–36. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Tanzer A, Amemiya CT, Kim CB and Stadler PF: Evolution of microRNAs located within hox gene clusters. J Exp Zool B Mol Dev Evol. 304:75–85. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Woltering JM and Durston AJ: Mir-10 represses hoxb1a and hoxb3a in zebrafish. PLoS One. 3:e13962008. View Article : Google Scholar : PubMed/NCBI

13 

Mu N, Gu J, Huang T, Zhang C, Shu Z, Li M, Hao Q, Li W, Zhang W, Zhao J, et al: A novel NF-κB/YY1/MicroRNA-10a regulatory circuit in fibroblast-like synoviocytes regulates inflammation in rheumatoid arthritis. Sci Rep. 6:200592016. View Article : Google Scholar : PubMed/NCBI

14 

Liang D, Zhen L, Yuan T, Huang J, Deng F, Wuyahan, Zhang H, Pan L, Liu Y, The E, et al: miR-10a regulates proliferation of human cardiomyocyte progenitor cells by targeting GATA6. PLoS One. 9:e1030972014. View Article : Google Scholar : PubMed/NCBI

15 

Xiong G, Huang H, Feng M, Yang G, Zheng S, You L, Zheng L, Hu Y, Zhang T and Zhao Y: miR-10a-5p targets TFAP2C to promote gemcitabine resistance in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res. 37:762018. View Article : Google Scholar : PubMed/NCBI

16 

Huang J, Zhao L, Xing L and Chen D: MicroRNA-204 regulates Runx2 protein expression and mesenchymal progenitor cell differentiation. Stem Cells. 28:357–364. 2010.PubMed/NCBI

17 

Inose H, Ochi H, Kimura A, Fujita K, Xu R, Sato S, Iwasaki M, Sunamura S, Takeuchi Y, Fukumoto S, et al: A microRNA regulatory mechanism of osteoblast differentiation. Proc Natl Acad Sci USA. 106:20794–20799. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative pcr and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

National Research Council, . Guide for the Care and Use of Laboratory Animals: Eighth Edition. The National Academies Press. (Washington, DC). 2011.

20 

Kanczler JM, Ginty PJ, Barry JJ, Clarke NM, Howdle SM, Shakesheff KM and Oreffo RO: The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation. Biomaterials. 29:1892–1900. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Reiser J, Zhang XY, Hemenway CS, Mondal D, Pradhan L and La Russa VF: Potential of mesenchymal stem cells in gene therapy approaches for inherited and acquired diseases. Expert Opin Biol Ther. 5:1571–1584. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Chen L, Xu Y, Zhao J, Zhang Z, Yang R, Xie J, Liu X and Qi S: Conditioned medium from hypoxic bone marrow-derived mesenchymal stem cells enhances wound healing in mice. PLoS One. 9:e961612014. View Article : Google Scholar : PubMed/NCBI

23 

Bernstein E, Caudy AA, Hammond SM and Hannon GJ: Role for a bidentateribonuclease in the initiation step of RNA interference. Nature. 409:363–366. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:806–811. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Morris KV and Mattick JS: The rise of regulatory RNA. Nat Rev Genet. 15:423–437. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Van Wijnen AJ, Stein GS, Gergen JP, Groner Y, Hiebert SW, Ito Y, Liu P, Neil JC, Ohki M and Speck N: Nomenclature for Runt-related (RUNX) proteins. Oncogene. 23:4209–4210. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Rosen CJ: Bone remodeling, energy metabolism, and the molecular clock. Cell Metab. 7:7–10. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Musumeci G, Mobasheri A, Trovato FM, Szychlinska MA, Graziano AC, Lo Furno D, Avola R, Mangano S, Giuffrida R and Cardile V: Biosynthesis of collagen I, II, RUNX2 and lubricin at different time points of chondrogenic differentiation in a 3D in vitro model of human mesenchymal stem cells derived from adipose tissue. Acta Histochem. 116:1407–1417. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Izu Y, Sun M, Zwolanek D, Veit G, Williams V, Cha B, Jepsen KJ, Koch M and Birk DE: Type XII collagen regulates osteoblast polarity and communication during bone formation. J Cell Biol. 193:1115–1130. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Liu Y, Xu F, Pei HX, Zhu X, Lin X, Song CY, Liang QH, Liao EY and Yuan LQ: Vaspin regulates the osteogenic differentiation of MC3T3-E1 through the Pi3K-Akt/miR-34c loop. Sci Rep. 6:255782016. View Article : Google Scholar : PubMed/NCBI

31 

Bonyadi Rad E, Musumeci G, Pichler K, Heidary M, Szychlinska MA, Castrogiovanni P, Marth E, Böhm C, Srinivasaiah S, Krönke G, et al: Runx2 mediated induction of novel targets ST2 and Runx3 leads to cooperative regulation of hypertrophic differentiation in ATDC5 chondrocytes. Sci Rep. 7:179472017. View Article : Google Scholar : PubMed/NCBI

32 

Zhang Y, Xie RL, Croce CM, Stein JL, Lian JB, Van Wijnen AJ and Stein GS: A program of microRNAs controls osteogenic lineage progression by targeting transcription factor Runx2. Proc Natl Acad Sci USA. 108:9863–9868. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Tian F, Ji XL, Xiao WA, Wang B and Wang F: CXCL13 promotes osteogenic differentiation of mesenchymal stem cells by inhibiting miR-23a expression. Stem Cells Int. 2015:6323052015. View Article : Google Scholar : PubMed/NCBI

34 

Chang CH, Fan TC, Yu JC, Liao GS, Lin YC, Shih AC, Li WH and Yu AL: The prognostic significance of RUNX2 and miR-10a/10b and their inter-relationship in breast cancer. J Transl Med. 12:2572014. View Article : Google Scholar : PubMed/NCBI

35 

Saltiel AR: Structural and functional roles of glycosylphosphoinositides. Subcell Biochem. 26:65–185. 1996.

36 

Wang X, Guo B, Li Q, Peng J, Yang Z, Wang A, Li D, Hou Z, Lv K, Kan G, et al: miR-214 targets ATF4 to inhibit bone formation. Nat Med. 19:93–100. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Liu XD, Cai F, Liu L, Zhang Y and Yang AL: MicroRNA-210 is involved in the regulation of postmenopausal osteoporosis through promotion of VEGF expression and osteoblast differentiation. Biol Chem. 396:339–347. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Shen E, Diao X, Wei C, Wu Z, Zhang L and Hu B: MicroRNAs target gene and signaling pathway by bioinformatics analysis in the cardiac hypertrophy. Biochem Biophys Res Commun. 397:380–385. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Cao Q, Li YY, He WF, Zhang ZZ, Zhou Q, Liu X, Shen Y and Huang TT: Interplay between microRNAs and the STAT3 signaling pathway in human cancers. Physiol Genomics. 45:1206–1214. 2013. View Article : Google Scholar : PubMed/NCBI

40 

Zhang W, Xie Y, Xu L, Wang Y, Zhu X, Wang R, Zhang Y, Muleke EM and Liu L: Identification of microRNAs and their target genes explores miRNA-mediated regulatory network of cytoplasmic male sterility occurrence during anther development in radish (Raphanus sativus L.). Front Plant Sci. 7:10542016.PubMed/NCBI

41 

Otto F, Lübbert M and Stock M: Upstream and downstream targets of RUNX proteins. J Cell Biochem. 89:9–18. 2003. View Article : Google Scholar : PubMed/NCBI

42 

Park J, Wada S, Ushida T and Akimoto T: The microRNA-23a has limited roles in bone formation and homeostasis in vivo. Physiol Res. 64:711–719. 2015. View Article : Google Scholar : PubMed/NCBI

43 

Taipaleenmä H, Eskildsen T, Stenvang J, Abdallah MB, Ditzel N, Säämäne AM, Kauppine S and Kasse M: Mir-138 is a novel regulator of in vivo bone formation. Bone. 47 (Suppl 1):S462010. View Article : Google Scholar

44 

Chen L, Holmstrøm K, Qiu W, Ditzel N, Shi K, Hokland L and Kassem M: MicroRNA-34a inhibits osteoblast differentiation and in vivo bone formation of human stromal stem cells. Stem Cells. 32:902–912. 2014. View Article : Google Scholar : PubMed/NCBI

45 

Liu L, Liu M, Li R, Liu H, Du L, Chen H, Zhang Y, Zhang S and Liu D: MicroRNA-503-5p inhibits stretch-induced osteogenic differentiation and bone formation. Cell Biol Int. 41:112–123. 2017. View Article : Google Scholar : PubMed/NCBI

46 

Hattori S: Structural features of ectopic bone-like tissue in porous hydroxyapatite blocks. Kokubyo Gakkai Zasshi. 75:120–137. 2008.(In Japanese). View Article : Google Scholar : PubMed/NCBI

47 

Mayr H, Schlüfter S, Detsch R and Ziegler G: Influence of phase composition on degradation and resorption of biphasic calcium phosphate ceramics. Key Eng Mater 361-363. 1043–1046. 2008.

48 

Daculsi G, Laboux O, Malard O and Weiss P: Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med. 14:195–200. 2003. View Article : Google Scholar : PubMed/NCBI

49 

Yamada S, Heymann D, Bouler JM and Daculsi G: Osteoclasticresorption of calcium phosphate ceramics with different hydroxyapatite/beta-tricalcium phosphate ratios. Biomaterials. 18:1037–1041. 1997. View Article : Google Scholar : PubMed/NCBI

50 

Rentsch C, Schneiders W, Manthey S, Rentsch B and Rammelt S: Comprehensive histological evaluation of bone implants. Biomatter. 4:e279932014. View Article : Google Scholar : PubMed/NCBI

51 

Eskildsen T, Taipaleenmäki H, Stenvang J, Abdallah BM, Ditzel N, Nossent AY, Bak M, Kauppinen S and Kassem M: MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo. Proc Natl Acad Sci USA. 108:6139–6144. 2011. View Article : Google Scholar : PubMed/NCBI

52 

Qadir AS, Um S, Lee H, Baek K, Seo BM, Lee G, Kim GS, Woo KM, Ryoo HM and Baek JH: miR-124 negatively regulates osteogenic differentiation and in vivo bone formation of mesenchymal stem cells. J Cell Biochem. 116:730–742. 2015. View Article : Google Scholar : PubMed/NCBI

53 

Liu S, Liu D, Chen C, Hamamura K, Moshaverinia A, Yang R, Liu Y, Jin Y and Shi S: MSC transplantation improves osteopenia via epigenetic regulation of notch signaling in lupus. Cell Metab. 22:606–618. 2015. View Article : Google Scholar : PubMed/NCBI

54 

Luo Y, Chen GL, Hannemann N, Ipseiz N, Krönke G, Bäuerle T, Munos L, Wirtz S, Schett G and Bozec A: Microbiota from obese mice regulate hematopoietic stem cell differentiation by altering the bone niche. Cell Metab. 22:886–894. 2015. View Article : Google Scholar : PubMed/NCBI

55 

Zou W, Greenblatt MB, Brady N, Lotinun S, Zhai B, de Rivera H, Singh A, Sun J, Gygi SP, Baron R, et al: The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2. J Exp Med. 210:1793–1806. 2013. View Article : Google Scholar : PubMed/NCBI

56 

Sureban SM, May R, Lightfoot SA, Hoskins AB, Lerner M, Brackett DJ, Postier RG, Ramanujam R, Mohammed A, Rao CV, et al: DCAMKL-1 regulates epithelial-mesenchymal transition in human pancreatic cells through a miR-200a-dependent mechanism. Cancer Res. 71:2328–2338. 2011. View Article : Google Scholar : PubMed/NCBI

57 

Sureban SM, May R, Ramalingam S, Subramaniam D, Natarajan G, Anant S and Houchen CW: Selective blockade of DCAMKL-1 results in tumor growth arrest by a Let-7a MicroRNA-dependent mechanism. Gastroenterology. 137:649–659. 2009. View Article : Google Scholar : PubMed/NCBI

58 

Cao T, Li H, Hu Y, Ma D and Cai X: miR-144 suppresses the proliferation and metastasis of hepatocellular carcinoma by targeting E2F3. Tumour Biol. 35:10759–10764. 2014. View Article : Google Scholar : PubMed/NCBI

59 

Fan Y, Hanai JI, Le PT, Bi R, Maridas D, DeMambro V, Figueroa CA, Kir S, Zhou X, Mannstadt M, et al: Parathyroid hormone directs bone marrow mesenchymal cell fate. Cell Metab. 25:661–672. 2017. View Article : Google Scholar : PubMed/NCBI

60 

Li JY, D'Amelio P, Robinson J, Walker LD, Vaccaro C, Luo T, Tyagi AM, Yu M, Reott M, Sassi F, et al: IL-17A is increased in humans with primary hyperparathyroidism and mediates PTH-induced bone loss in mice. Cell Metab. 22:799–810. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhou L, Zhang Z, Ren F, Chen L and Lan Z: miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Mol Med Rep 22: 135-144, 2020.
APA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., & Lan, Z. (2020). miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Molecular Medicine Reports, 22, 135-144. https://doi.org/10.3892/mmr.2020.11110
MLA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22.1 (2020): 135-144.
Chicago
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22, no. 1 (2020): 135-144. https://doi.org/10.3892/mmr.2020.11110
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhou L, Zhang Z, Ren F, Chen L and Lan Z: miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Mol Med Rep 22: 135-144, 2020.
APA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., & Lan, Z. (2020). miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells. Molecular Medicine Reports, 22, 135-144. https://doi.org/10.3892/mmr.2020.11110
MLA
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22.1 (2020): 135-144.
Chicago
Zhang, Y., Zhou, L., Zhang, Z., Ren, F., Chen, L., Lan, Z."miR‑10a‑5p inhibits osteogenic differentiation of bone marrow‑derived mesenchymal stem cells". Molecular Medicine Reports 22, no. 1 (2020): 135-144. https://doi.org/10.3892/mmr.2020.11110
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team