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Article

Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells

  • Authors:
    • Zuhal Eroglu
    • Ceren Erdem
    • Gulperi Oktem
    • Vildan Bozok Cetintas
    • Zekeriya Duzgun
  • View Affiliations / Copyright

    Affiliations: Department of Medical Biology, Faculty of Medicine, Ege University, Bornova, Izmir 35100, Turkey, Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, Izmir 35100, Turkey, Department of Medical Biology, Faculty of Medicine, Giresun University, Debboy, Giresun 28100, Turkey
  • Pages: 575-581
    |
    Published online on: May 4, 2020
       https://doi.org/10.3892/mmr.2020.11113
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Abstract

Lung cancer is one of the most commonly diagnosed cancers and it is associated with high rates of morbidity and mortality. Metastasis and relapse of the tumor depend on the survival and proliferation of lung cancer stem cells (LCSCs). The ability to identify CSCs may prevent recurrence and lead to more effective treatments. Sirtuins are a group of deacetylases that include seven variants (SIRT1‑7), with sirtuin 1 (SIRT1) being the most intensively investigated. Evidence suggests that SIRT1 is both a tumor‑suppressor gene and an oncogene. SIRT1 can deacetylate the tumor‑suppressor protein p53 to decrease its activity. SIRT1 activators increase the deacetylation of p53, whereas SIRT1 inhibitors can stimulate p53 by inhibiting deacetylation. In the present study, CD44+ and CD133+‑enriched A549 (non‑small cell lung cancer) cells collected using the CD44 and CD133 CSC surface markers by fluorescence‑activated cell sorting method were treated with SIRT1 inhibitors (tenovin‑6 and sirtinol) and SIRT1 activators (resveratrol and SRT1720), and their effects on apoptosis, as well as the mRNA and protein expression of SIRT1 and p53 were investigated. Of these agents, it was found that resveratrol increased p53 expression by 4.1‑fold, decreased SIRT1 expression by 0.2‑fold, and it was the most potent inducer of apoptosis.
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View References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Zappa C and Mousa SA: Non-small cell lung cancer: Current treatment and future advances. Transl Lung Cancer Res. 5:288–300. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Rich JN: Cancer stem cells: Understanding tumor hierarchy and heterogeneity. Medicine (Baltimore). 95 (1 Suppl 1):S2–S7. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Leeman KT, Fillmore CM and Kim CF: Lung stem and progenitor cells in tissue homeostasis and disease. Curr Top Dev Biol. 107:207–233. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Salama R, Tang J, Gadgeel SM, Ahmad A and Sarkar FH: Lung cancer stem cells: Current progress and future perspectives. J Stem Cell Res Ther S7. 0072012.

6 

Luo J, Zhou X and Yakisich JS: Stemness and plasticity of lung cancer cells: Paving the road for better therapy. Onco Targets Ther. 7:1129–1134. 2014.PubMed/NCBI

7 

Wang Y, Jiang M, Du C, Yu Y, Liu Y, Li M and Luo F: Utilization of lung cancer cell lines for the study of lung cancer stem cells. Oncol Lett. 15:6791–6798. 2018.PubMed/NCBI

8 

Halim NHA, Zakaria N, Satar NA and Yahaya BH: Isolation and characterization of cancer stem cells of the non-small-cell lung cancer (A549) cell line. Methods Mol Biol. 1516:371–388. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Haigis MC and Sinclair DA: Mammalian sirtuins: Biological insights and disease relevance. Annu Rev Pathol. 5:253–295. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Mei Z, Zhang X, Yi J, Huang J, He J and Tao Y: Sirtuins in metabolism, DNA repair and cancer. J Exp Clin Cancer Res. 35:1822016. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Zhang HZ, Lin XG, Hua P, Wang M, Ao X, Xiong LH, Wu C and Guo JJ: The study of the tumor stem cell properties of CD133+CD44+ cells in the human lung adenocarcinoma cell line A549. Cell Mol Biol (Noisy-le-grand). 56 (Suppl):OL1350–OL1358. 2010.PubMed/NCBI

13 

Leung EL, Fiscus RR, Tung JW, Tin VP, Cheng LC, Sihoe AD, Fink LM, Ma Y and Wong MP: Non-small cell lung cancer cells expressing CD44 are enriched for stem cell-like properties. PLoS One. 5:e140622010. View Article : Google Scholar : PubMed/NCBI

14 

Tirino V, Camerlingo R, Franco R, Malanga D, La Rocca A, Viglietto G, Rocco G and Pirozzi G: The role of CD133 in the identification and characterisation of tumour-initiating cells in non-small-cell lung cancer. Eur J Cardiothorac Surg. 36:446–453. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Chao SC, Chen YJ, Huang KH, Kuo KL, Yang TH, Huang KY, Wang CC, Tang CH, Yang RS and Liu SH: Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity. Sci Rep. 7:31802017. View Article : Google Scholar : PubMed/NCBI

16 

Wang J, Li J, Cao N, Li Z, Han J and Li L: Resveratrol, an activator of SIRT1, induces protective autophagy in non-small-cell lung cancer via inhibiting Akt/mTOR and activating p38-MAPK. Onco Targets Ther. 11:7777–7786. 2018. View Article : Google Scholar : PubMed/NCBI

17 

Zhang X, Wan F, You W, Tan X, Liu G, Jin Q, Wei C, Liu X, Zhao H, Liu Y and Zhang C: Comparison of apoptosis between bovine subcutaneous and intramuscular adipocytes by resveratrol via SIRT1. Anim Biotechnol. 1–9. 2019. View Article : Google Scholar

18 

Yun JM, Chien A, Jialal I and Devaraj S: Resveratrol up-regulates SIRT1 and inhibits cellular oxidative stress in the diabetic milieu: Mechanistic insights. J Nutr Biochem. 23:699–705. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chai R, Fu H, Zheng Z, Liu T, Ji S and Li G: Resveratrol inhibits proliferation and migration through SIRT1 mediated post-translational modification of PI3K/AKT signaling in hepatocellular carcinoma cells. Mol Med Rep. 16:8037–8044. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Frazzi R, Valli R, Tamagnini I, Casali B, Latruffe N and Merli F: Resveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation. Int J Cancer. 132:1013–1021. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Pacholec M, Bleasdale JE, Chrunyk B, Cunningham D, Flynn D, Garofalo RS, Griffith D, Griffor M, Loulakis P, Pabst B, et al: SRT1720, SRT2183, SRT1460, and resveratrol are not direct activators of SIRT1. J Biol Chem. 285:8340–8351. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Bi X, Ye Q, Li D, Peng Q, Wang Z, Wu X, Zhang Y, Zhang Q and Jiang F: Inhibition of nucleolar stress response by Sirt1: A potential mechanism of acetylation-independent regulation of p53 accumulation. Aging Cell. 18:e129002019. View Article : Google Scholar : PubMed/NCBI

23 

Wang X, Wang D and Zhao Y: Effect and mechanism of resveratrol on the apoptosis of lung adenocarcinoma cell line A549. Cell Biochem Biophys. 73:527–531. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Yuan L, Zhang Y, Xia J, Liu B, Zhang Q, Liu J, Luo L, Peng Z, Song Z and Zhu R: Resveratrol induces cell cycle arrest via a p53-independent pathway in A549 cells. Mol Med Rep. 11:2459–2464. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Lahusen TJ and Deng CX: SRT1720 induces lysosomal-dependent cell death of breast cancer cells. Mol Cancer Ther. 14:183–192. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Hirai S, Endo S, Saito R, Hirose M, Ueno T, Suzuki H, Yamato K, Abei M and Hyodo I: Antitumor effects of a sirtuin inhibitor, tenovin-6, against gastric cancer cells via death receptor 5 up-regulation. PLoS One. 9:e1028312014. View Article : Google Scholar : PubMed/NCBI

27 

Ueno T, Endo S, Saito R, Hirose M, Hirai S, Suzuki H, Yamato K and Hyodo I: The sirtuin inhibitor tenovin-6 upregulates death receptor 5 and enhances cytotoxic effects of 5-fluorouracil and oxaliplatin in colon cancer cells. Oncol Res. 21:155–164. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Wang J, Kim TH, Ahn MY, Lee J, Jung JH, Choi WS, Lee BM, Yoon KS, Yoon S and Kim HS: Sirtinol, a class III HDAC inhibitor, induces apoptotic and autophagic cell death in MCF-7 human breast cancer cells. Int J Oncol. 41:1101–1109. 2012. View Article : Google Scholar : PubMed/NCBI

29 

MacCallum SF, Groves MJ, James J, Murray K, Appleyard V, Prescott AR, Drbal AA, Nicolaou A, Cunningham J, Haydock S, et al: Dysregulation of autophagy in chronic lymphocytic leukemia with the small-molecule sirtuin inhibitor tenovin-6. Sci Rep. 3:12752013. View Article : Google Scholar : PubMed/NCBI

30 

Solomon JM, Pasupuleti R, Xu L, McDonagh T, Curtis R, DiStefano PS and Huber LJ: Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage. Mol Cell Biol. 26:28–38. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Eroglu Z, Erdem C, Oktem G, Bozok Cetintas V and Duzgun Z: Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells. Mol Med Rep 22: 575-581, 2020.
APA
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., & Duzgun, Z. (2020). Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells. Molecular Medicine Reports, 22, 575-581. https://doi.org/10.3892/mmr.2020.11113
MLA
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., Duzgun, Z."Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells". Molecular Medicine Reports 22.1 (2020): 575-581.
Chicago
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., Duzgun, Z."Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells". Molecular Medicine Reports 22, no. 1 (2020): 575-581. https://doi.org/10.3892/mmr.2020.11113
Copy and paste a formatted citation
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Spandidos Publications style
Eroglu Z, Erdem C, Oktem G, Bozok Cetintas V and Duzgun Z: Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells. Mol Med Rep 22: 575-581, 2020.
APA
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., & Duzgun, Z. (2020). Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells. Molecular Medicine Reports, 22, 575-581. https://doi.org/10.3892/mmr.2020.11113
MLA
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., Duzgun, Z."Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells". Molecular Medicine Reports 22.1 (2020): 575-581.
Chicago
Eroglu, Z., Erdem, C., Oktem, G., Bozok Cetintas, V., Duzgun, Z."Effect of SIRT1 activators and inhibitors on CD44+/CD133+‑enriched non‑small cell lung cancer cells". Molecular Medicine Reports 22, no. 1 (2020): 575-581. https://doi.org/10.3892/mmr.2020.11113
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