Open Access

Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models

  • Authors:
    • Yi Zhu
    • Jie Cheng
    • Jichao Yin
    • Yujie Yang
    • Jiabao Guo
    • Wenyi  Zhang
    • Bing Xie
    • Haixia Lu
    • Dingjun Hao
  • View Affiliations

  • Published online on: May 15, 2020     https://doi.org/10.3892/mmr.2020.11148
  • Pages: 763-773
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Spinal cord injury (SCI) often leads to defecation dysfunction. Sacral nerve electrical stimulation (SNS) therapy could improve defecation function. The present study aimed to assess SNS therapy, with regard to the levels of serotonin (5‑HT) and its receptors (5‑HT3AR and 5‑HT4R) in the colon and sacral cord, a rat model of acute severe SCI was used. This rat model was made using the New York University Impactor device. Model rats were randomized to the SCI and SNS (electrical stimulation on the S3 nerve) groups. After 14 days of treatment, enteric transmission function was assessed. 5‑HT and 5‑HT3AR/5‑HT4R were measured by ELISA, quantitative PCR, immunohistochemistry and western blotting. In SCI rats, SNS significantly increased the quantity of feces, shortened the time to the first fecal passage, and improved fecal texture and colon histology. SNS elevated 5‑HT contents in the colon and spinal cord, and enhanced 5‑HT3AR/5‑HT4R protein expression and distribution in the colonic myenteric plexus and mucosa, sacral intermediolateral nucleus and dorsal horn. SNS upregulated the relative expression levels of 5‑HT3AR/5‑HT4R mRNA and protein in the colon and spinal cord. SNS can improve defecation and accelerate the recovery of colonic transmission functions in rat models of acute SCI. These effects involved upregulation of the 5‑HT/5‑HT3AR/5‑HT4R axes.

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APA
Zhu, Y., Cheng, J., Yin, J., Yang, Y., Guo, J., Zhang, W. ... Hao, D. (2020). Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models. Molecular Medicine Reports, 22, 763-773. https://doi.org/10.3892/mmr.2020.11148
MLA
Zhu, Y., Cheng, J., Yin, J., Yang, Y., Guo, J., Zhang, W., Xie, B., Lu, H., Hao, D."Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models". Molecular Medicine Reports 22.2 (2020): 763-773.
Chicago
Zhu, Y., Cheng, J., Yin, J., Yang, Y., Guo, J., Zhang, W., Xie, B., Lu, H., Hao, D."Effects of sacral nerve electrical stimulation on 5‑HT and 5‑HT3AR/5‑HT4R levels in the colon and sacral cord of acute spinal cord injury rat models". Molecular Medicine Reports 22, no. 2 (2020): 763-773. https://doi.org/10.3892/mmr.2020.11148