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Article Open Access

Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis

  • Authors:
    • Fei Yan
    • Yue Feng
    • Jin Chen
    • Jing Yan
  • View Affiliations / Copyright

    Affiliations: Department of General Practice, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Radiology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Critical Care Medicine, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1035-1043
    |
    Published online on: May 22, 2020
       https://doi.org/10.3892/mmr.2020.11178
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Abstract

Klotho is a type of single‑pass transmembrane protein that is important for the proper function of numerous organs. The aim of the present study was to investigate the role of Klotho in sepsis‑associated myocardial damage. In the present study, reverse transcription‑quantitative PCR, western blotting and ELISA were conducted to examine the expression levels of function genes, and flow cytometry was performed to detect cell apoptosis and reactive oxygen species. The present study demonstrated that Klotho expression was significantly downregulated in septic mice and that the myocardial function of septic mice improved after treatment with exogenous Klotho protein. It further demonstrated that indoxyl sulfate inhibited the expression of Klotho protein. In addition, decreased Klotho protein further led to activation of the reactive oxygen species‑p38 mitogen‑activated protein kinase signaling pathway, finally resulting in myocardial damage. In conclusion, Klotho protein may be a key regulator in the myocardial damage of cardiorenal syndrome in sepsis. It also has a potential to be a therapeutic target for sepsis‑associated myocardial damage in the future.
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1 

Krivan S, Kapelouzou A, Vagios S, Tsilimigras D, Katsimpoulas M, Moris D, Aravanis CV, Demesticha TD, Schizas D, Mavroidis M, et al: Increased expression of Toll-like receptors 2, 3, 4 and 7 mRNA in the kidney and intestine of a septic mouse model. Sci Rep. 9:40102019. View Article : Google Scholar : PubMed/NCBI

2 

Aird WC: The role of the endothelium in severe sepsis and multiple organ dysfunction syndrome. Blood. 101:3765–3777. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Virzi GM, Clementi A, Brocca A and Ronco C: Endotoxin effects on cardiac and renal functions and cardiorenal syndromes. Blood Purif. 44:314–326. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Kotecha A, Vallabhajosyula S, Coville HH and Kashani K: Cardiorenal syndrome in sepsis: A narrative review. J Crit Care. 43:122–127. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Virzi GM, Clementi A, Brocca A, de Cal M, Marcante S and Ronco C: Cardiorenal syndrome type 5 in sepsis: Role of endotoxin in cell death pathways and inflammation. Kidney Blood Press Res. 41:1008–1015. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Clementi A, Virzi GM, Brocca A and Ronco C: The role of endotoxin in the setting of cardiorenal syndrome type 5. Cardiorenal Med. 7:276–283. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Blanco J, Muriel-Bombín A, Sagredo V, Taboada F, Gandía F, Tamayo L, Collado J, García-Labattut A, Carriedo D, Valledor M, et al: Incidence, organ dysfunction and mortality in severe sepsis: A Spanish multicentre study. Critic care. 12:R1582008. View Article : Google Scholar

8 

Abolghasemi M, Yousefi T, Maniati M and Qujeq D: The interplay of Klotho with signaling pathway and microRNAs in cancers. J Cell Biochem. 120:14306–14317. 2019. View Article : Google Scholar : PubMed/NCBI

9 

Olejnik A, Franczak A, Krzywonos-Zawadzka A, Kaluzna-Oleksy M and Bil-Lula I: The biological role of klotho protein in the development of cardiovascular diseases. Biomed Res Int. 2018:51719452018. View Article : Google Scholar : PubMed/NCBI

10 

Oh HJ, Oh H, Nam BY, You JS, Ryu DR, Kang SW and Chung YE: The protective effect of klotho against contrast-associated acute kidney injury via the anti-oxidative effect. Am J Physiol Renal Physiol. 317:F881–F889. 2019. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Hu MC, Shi M, Gillings N, Flores B, Takahashi M, Kuro-O M and Moe OW: Recombinant α-Klotho may be prophylactic and therapeutic for acute to chronic kidney disease progression and uremic cardiomyopathy. Kidney Int. 91:1104–1114. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Veldeman L, Vanmassenhove J, Van Biesen W, Massy ZA, Liabeuf S, Glorieux G and Vanholder R: Evolution of protein-bound uremic toxins indoxyl sulphate and p-cresyl sulphate in acute kidney injury. Int Urol Nephrol. 51:293–302. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Yu D, Li M, Tian Y, Liu J and Shang J: Luteolin inhibits ROS-activated MAPK pathway in myocardial ischemia/reperfusion injury. Life Sci. 122:15–25. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Lu S, Zhang Y, Zhong S, Gao F, Chen Y, Li W, Zheng F and Shi G: N-n-butyl haloperidol iodide protects against hypoxia/reoxygenation injury in cardiac microvascular endothelial cells by regulating the ROS/MAPK/Egr-1 pathway. Front Pharmacol. 7:5202016.PubMed/NCBI

16 

Zanotti-Cavazzoni SL and Hollenberg SM: Cardiac dysfunction in severe sepsis and septic shock. Curr Opin Crit Care. 15:392–397. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Yousif NG, Hadi NR and Hassan AM: Indocyanine green-001 (ICG-001) attenuates Wnt/β-catenin-induces myocardial injury following sepsis. J Pharmacol Pharmacother. 8:14–20. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Zheng G, Pan M, Jin W, Jin G and Huang Y: MicroRNA-135a is up-regulated and aggravates myocardial depression in sepsis via regulating p38 MAPK/NF-κB pathway. Int Immunopharmacol. 45:6–12. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Li Z, Zhang E, Hu Y, Liu Y and Chen B: High Serum sTREM-1 correlates with myocardial dysfunction and predicts prognosis in septic patients. Am J Med Sci. 351:555–562. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Kuro-o M, Matsumura Y, Aizawa H, Kawaguchi H, Suga T, Utsugi T, Ohyama Y, Kurabayashi M, Kaname T, Kume E, et al: Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Nature. 390:45–51. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Reish NJ, Maltare A, McKeown AS, Laszczyk AM, Kraft TW, Gross AK and King GD: The age-regulating protein klotho is vital to sustain retinal function. Invest Ophthalmol Vis Sci. 54:6675–6685. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Xu Y and Sun Z: Molecular basis of klotho: From gene to function in aging. Endocr Rev. 36:174–193. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Flotyńska J, Uruska A, Araszkiewicz A and Zozulińska-Ziółkiewicz D: Klotho protein function among patients with type 1 diabetes. Endokrynol Pol. 69:696–704. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Guo Y, Zhuang X, Huang Z, Zou J, Yang D, Hu X, Du Z, Wang L and Liao X: Klotho protects the heart from hyperglycemia-induced injury by inactivating ROS and NF-κB-mediated inflammation both in vitro and in vivo. Biochim Biophys Acta Mol Basis Dis. 1864:238–251. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Semba RD, Cappola AR, Sun K, Bandinelli S, Dalal M, Crasto C, Guralnik JM and Ferrucci L: Plasma klotho and cardiovascular disease in adults. J Am Geriatrics Soc. 59:1596–1601. 2011. View Article : Google Scholar

26 

Xie J, Cha SK, An SW, Kuro OM, Birnbaumer L and Huang CL: Cardioprotection by Klotho through downregulation of TRPC6 channels in the mouse heart. Nat Commun. 3:12382012. View Article : Google Scholar : PubMed/NCBI

27 

Lin YT, Wu PH, Tsai YC, Hsu YL, Wang HY, Kuo MC, Kuo PL and Hwang SJ: Indoxyl sulfate induces apoptosis through oxidative stress and mitogen-activated protein kinase signaling pathway inhibition in human astrocytes. J Clin Med. 8(pii): E1912019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yan F, Feng Y, Chen J and Yan J: Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis. Mol Med Rep 22: 1035-1043, 2020.
APA
Yan, F., Feng, Y., Chen, J., & Yan, J. (2020). Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis. Molecular Medicine Reports, 22, 1035-1043. https://doi.org/10.3892/mmr.2020.11178
MLA
Yan, F., Feng, Y., Chen, J., Yan, J."Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis". Molecular Medicine Reports 22.2 (2020): 1035-1043.
Chicago
Yan, F., Feng, Y., Chen, J., Yan, J."Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis". Molecular Medicine Reports 22, no. 2 (2020): 1035-1043. https://doi.org/10.3892/mmr.2020.11178
Copy and paste a formatted citation
x
Spandidos Publications style
Yan F, Feng Y, Chen J and Yan J: Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis. Mol Med Rep 22: 1035-1043, 2020.
APA
Yan, F., Feng, Y., Chen, J., & Yan, J. (2020). Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis. Molecular Medicine Reports, 22, 1035-1043. https://doi.org/10.3892/mmr.2020.11178
MLA
Yan, F., Feng, Y., Chen, J., Yan, J."Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis". Molecular Medicine Reports 22.2 (2020): 1035-1043.
Chicago
Yan, F., Feng, Y., Chen, J., Yan, J."Klotho downregulation contributes to myocardial damage of cardiorenal syndrome in sepsis". Molecular Medicine Reports 22, no. 2 (2020): 1035-1043. https://doi.org/10.3892/mmr.2020.11178
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