The gonadal expression pattern of lipocalin‑2 and 24p3 receptor is modified in the gonads of the offspring of obese rats

De La Chesnaye,Elsa; Manuel‑Apolinar,Leticia; Damasio,Leticia; Castrejón,Edgar; López‑Ballesteros,Rebeca; Revilla‑Monsalve,María   Cristina; Méndez,Juan   Pablo

doi:10.3892/mmr.2020.11226

The gonadal expression pattern of lipocalin‑2 and 24p3 receptor is modified in the gonads of the offspring of obese rats

Authors:
- Elsa De La Chesnaye
- Leticia Manuel‑Apolinar
- Leticia Damasio
- Edgar Castrejón
- Rebeca López‑Ballesteros
- María Cristina Revilla‑Monsalve
- Juan Pablo Méndez
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Affiliations: Cardiovascular and Metabolic Diseases Research Unit, Mexican Social Security Institute, Mexico City 06720, Mexico, Endocrine Research Unit, National Medical Center, Mexican Social Security Institute, Mexico City 06720, Mexico , Department of Biochemistry Diagnostics, Faculty of Higher Education, Cuautitlán Izcalli Campus, National Autonomous University of Mexico, State of Mexico 54714, Mexico, Peripheral Obesity Research Unit, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 14000, Mexico
Published online on: June 12, 2020 https://doi.org/10.3892/mmr.2020.11226
Pages: 1409-1419
Copyright: © De La Chesnaye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Obesity represents a global health and economic burden, affecting millions of individuals worldwide. This pathology is associated with a chronic low‑grade inflammatory state that is partially responsible for the development of other cardiometabolic complications. Clinical studies have reported an association between high circulating levels of lipocalin‑2 (Lcn2) and increased body weight. Additionally, there is scientific evidence demonstrating the impact of maternal obesity on fetal programming. The latter and the fact that the authors previously found that Lcn2 and its receptor (24p3R) are expressed in the gonads of wild‑type rats, led to the analysis of their mRNA profile and cellular localization in gonads collected from the offspring of obese rats at 21 days postconception (dpc), and 0, 2, 4, 6, 12, 20 and 30 days postnatal (dpn) in the present study. Semi‑quantitative PCR revealed a statistically significant downregulation of Lcn2 and 24p3R mRNA at 21 dpc in the ovaries (P<0.01) and testicles (P<0.001) of the offspring of obese mothers. At 30 dpn, the relative expression of Lcn2 mRNA decreased significantly in the ovaries of the experimental group (P<0.05), while Lcn2 mRNA expression was not detectable in testicles. Regarding 24p3R, its mRNA was only significantly decreased at 21 dpc in ovaries of pups of obese mothers. At 30 dpn, the change in females was not significant. Conversely, in testicles, 24p3R mRNA levels increased slightly in the experimental group at 30 dpn. The Lcn2 protein signal was less intense in gonadal tissue sections from 30 dpn offspring of obese rats (P<0.001), whereas the 24p3R signal was downregulated in ovaries (P<0.001) and slightly upregulated in testicles. It was concluded that maternal obesity changes the expression of Lcn2 and 24p3R in the gonads of the offspring of obese rats, possibly through fetal programming. The consequences of this dysregulation for the offspring's gonadal function remains to be determined.

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