Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src‑dependent pathway in MDA‑MB‑231 breast cancer cells

Ramírez‑Ricardo,Javier; Leal‑Orta,Elizabeth; Martínez‑Baeza,Elia; Ortiz‑Mendoza,Carlos; Breton‑Mora,Fernando; Herrera‑Torres,Analy; Elizalde‑Acosta,Irma; Cortes‑Reynosa,Pedro; Thompson‑Bonilla,Rocio; Perez Salazar,Eduardo

doi:10.3892/mmr.2020.11259

Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src‑dependent pathway in MDA‑MB‑231 breast cancer cells

Authors:
- Javier Ramírez‑Ricardo
- Elizabeth Leal‑Orta
- Elia Martínez‑Baeza
- Carlos Ortiz‑Mendoza
- Fernando Breton‑Mora
- Analy Herrera‑Torres
- Irma Elizalde‑Acosta
- Pedro Cortes‑Reynosa
- Rocio Thompson‑Bonilla
- Eduardo Perez Salazar
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Affiliations: Departament of Cell Biology, CINVESTAV‑IPN, Mexico City 07360, Mexico, General Hospital Tacuba‑ISSSTE, Mexico City 11410, Mexico, General Hospital Dr. Fernando Quiroz‑ISSSTE, Mexico City 01140, Mexico, First October Regional Hospital‑ISSSTE, Mexico City 07760, Mexico
Published online on: June 18, 2020 https://doi.org/10.3892/mmr.2020.11259
Pages: 1932-1948
Copyright: © Ramírez‑Ricardo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Triple negative breast cancer (TNBC) is a breast cancer subtype associated with high rates of metastasis, heterogeneity, drug resistance and a poor prognosis. Extracellular vesicles (EVs) are vesicles of endosomal and plasma membrane origin, and are secreted by healthy and cancer cells. In cancer, EVs contribute to tumor progression by mediating escape from the immune system surveillance, and are involved in extracellular matrix degradation, invasion, angiogenesis, migration and metastasis. Furthermore, EVs have been identified in several human fluids. However, the role of EVs from patients with breast cancer in the migration and invasion of human breast cancer cells is not fully understood. The present study investigated whether EVs isolated from Mexican patients with breast cancer can induce cellular processes related to invasion in breast cancer. Moreover, plasma fractions enriched in EVs and deprived of platelet‑derived EVs obtained from blood samples of 32 Mexican patients with biopsy‑diagnosed breast cancer at different clinical stages who had not received treatment were analyzed. Furthermore, one control group was included, which consisted of 20 Mexican healthy females. The present results demonstrated that EVs from women with breast cancer promote migration and invasion, and increase matrix metalloproteinase (MMP)‑2 and MMP‑9 secretion in TNBC MDA‑MB‑231 cells. In addition, it was found that EVs from patients with breast cancer induced Src and focal adhesion kinase activation, and focal adhesions assembly with an increase in focal adhesions number, while the migration and invasion was dependent on Src activity. Collectively, EVs from Mexican patients with breast cancer induce migration and invasion via a Src‑dependent pathway in TNBC MDA‑MB‑231 cells.

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