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Article Open Access

Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2

  • Authors:
    • Shuli Shao
    • Shanshan Li
    • Chang Liu
    • Weiwei Zhang
    • Zhenzhu Zhang
    • Shaowei Zhu
    • Yunjianan Feng
    • Yang Pan
  • View Affiliations / Copyright

    Affiliations: Department of Life Science and Agroforestry, Qiqihar University, Qiqihar, Heilongjiang 161006, P.R. China
    Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1793-1802
    |
    Published online on: June 22, 2020
       https://doi.org/10.3892/mmr.2020.11263
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Abstract

Toosendanin (TSN) is a tetracyclic triterpenoid extracted from Melia toosendan Sieb, et Zucc, which primarily grows in specific areas of China. Although toosendanin (TSN) exerts antitumoral effects on various human cancer cells, its influence on gastric cancer (GC) is remains to be elucidated. MicroRNAs (miRNAs/miRs) serve crucial roles in apoptosis and proliferation of cancer cells. miR‑23a‑3p has been shown to be associated with human GC; however, the specific function of miR‑23a‑3p in GC remains unclear. Therefore, the present study aimed to elucidate the role of miR‑23a‑3p in the regulation of GC cell proliferation and apoptosis induced in vitro by TSN treatment. Subsequently, apoptosis‑related genes expression levels were quantified by reverse transcription‑quantitative PCR and western blot analysis, respectively, and the target relationship between miR‑23a‑3p and BCL2 was determined by luciferase reporter gene analysis. Additionally, cell proliferation and apoptosis experiments were carried out. The results indicated that TSN inhibited proliferation and induced apoptosis in MKN‑45 cells. Moreover, it upregulated the expression of miR‑23a‑3p. B‑cell lymphoma‑2 (BCL2) was identified as a potential target gene of miR‑23a‑3p, which was demonstrated to bind to the 3'‑untranslated region of BCL2 mRNA, as detected by the luciferase reporter assay. Further studies revealed that BCL2 expression was downregulated following overexpression of miR‑23a‑3p. In addition, the overexpression of the miR‑23a‑3p inhibited proliferation, induced G1 arrest and increased apoptosis in MKN‑45 cells. The results of the present study demonstrated that miR‑23a‑3p inhibited proliferation and induced apoptosis of GC cells, which may be attributable to its direct targeting of BCL2. These results may provide a novel insight into the apoptosis of GC cells, and may lead to investigations into the mechanisms of the effects of TSN.
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Copy and paste a formatted citation
Spandidos Publications style
Shao S, Li S, Liu C, Zhang W, Zhang Z, Zhu S, Feng Y and Pan Y: Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 . Mol Med Rep 22: 1793-1802, 2020.
APA
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S. ... Pan, Y. (2020). Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 . Molecular Medicine Reports, 22, 1793-1802. https://doi.org/10.3892/mmr.2020.11263
MLA
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S., Feng, Y., Pan, Y."Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 ". Molecular Medicine Reports 22.3 (2020): 1793-1802.
Chicago
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S., Feng, Y., Pan, Y."Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 ". Molecular Medicine Reports 22, no. 3 (2020): 1793-1802. https://doi.org/10.3892/mmr.2020.11263
Copy and paste a formatted citation
x
Spandidos Publications style
Shao S, Li S, Liu C, Zhang W, Zhang Z, Zhu S, Feng Y and Pan Y: Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 . Mol Med Rep 22: 1793-1802, 2020.
APA
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S. ... Pan, Y. (2020). Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 . Molecular Medicine Reports, 22, 1793-1802. https://doi.org/10.3892/mmr.2020.11263
MLA
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S., Feng, Y., Pan, Y."Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 ". Molecular Medicine Reports 22.3 (2020): 1793-1802.
Chicago
Shao, S., Li, S., Liu, C., Zhang, W., Zhang, Z., Zhu, S., Feng, Y., Pan, Y."Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2 ". Molecular Medicine Reports 22, no. 3 (2020): 1793-1802. https://doi.org/10.3892/mmr.2020.11263
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