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Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes

  • Authors:
    • Zi Zeng
    • Hui‑Min Liu
    • Yun‑Yan Zhang
    • Rong Chen
    • Tao Sun
    • Wei Li
    • Qian Sun
    • Zhong‑Yuan Xia
    • Qing‑Tao Meng
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1892-1900
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    Published online on: June 24, 2020
       https://doi.org/10.3892/mmr.2020.11270
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Abstract

Numerous studies have reported that diabetes is associated with an increased susceptibility to cardiac ischemia‑ reperfusion injury; however, the mechanism underlying the role of diabetes during intestinal ischemia‑reperfusion (IIR) has yet to be elucidated. The present study evaluated the intestinal pathological alterations and possible underlying mechanisms in a mouse model of type 1 diabetes mellitus with IIR. The effects of diabetes were investigated by assessing the histopathology, oxidative stress, inflammatory cytokine levels in intestine tissues and blood plasma, and protein expression levels of phosphatase and tensin homolog‑induced putative kinase (PINK1), Parkin and the ratio of light chain 3B (LC3B) II/I. The results demonstrated that diabetes increased the Chiu's intestinal injury score, concentration of interleukin (IL)‑1β, IL‑6 and tumor necrosis factor (TNF)‑α, and levels of oxidative stress. Furthermore, the alterations were more pronounced in the diabetes with IIR group. The expression levels of PINK1 and Parkin, as well as the ratio of LC3BII/I, were significantly upregulated in the IIR group compared with the Sham group. Diabetes activated PINK1 and Parkin, and increased the expression of LC3BII. Furthermore, transmission electron microscopy revealed that mitochondrial destruction and the number of autophagosomes was increased in the diabetic groups compared with the non‑diabetic groups. Collectively, the results of the present study suggest that diabetes increased intestinal vulnerability to IIR by enhancing inflammation and oxidative stress. Furthermore, IIR was associated with overactivation of mitochondrial autophagy; therefore, the increased vulnerability to IIR‑induced intestine damage due to diabetes may be associated with PINK1/Parkin‑regulated mitochondrial autophagy.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng Z, Liu HM, Zhang YY, Chen R, Sun T, Li W, Sun Q, Xia ZY and Meng QT: Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes. Mol Med Rep 22: 1892-1900, 2020.
APA
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W. ... Meng, Q. (2020). Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes. Molecular Medicine Reports, 22, 1892-1900. https://doi.org/10.3892/mmr.2020.11270
MLA
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W., Sun, Q., Xia, Z., Meng, Q."Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes". Molecular Medicine Reports 22.3 (2020): 1892-1900.
Chicago
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W., Sun, Q., Xia, Z., Meng, Q."Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes". Molecular Medicine Reports 22, no. 3 (2020): 1892-1900. https://doi.org/10.3892/mmr.2020.11270
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng Z, Liu HM, Zhang YY, Chen R, Sun T, Li W, Sun Q, Xia ZY and Meng QT: Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes. Mol Med Rep 22: 1892-1900, 2020.
APA
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W. ... Meng, Q. (2020). Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes. Molecular Medicine Reports, 22, 1892-1900. https://doi.org/10.3892/mmr.2020.11270
MLA
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W., Sun, Q., Xia, Z., Meng, Q."Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes". Molecular Medicine Reports 22.3 (2020): 1892-1900.
Chicago
Zeng, Z., Liu, H., Zhang, Y., Chen, R., Sun, T., Li, W., Sun, Q., Xia, Z., Meng, Q."Aggravated intestinal ischemia‑reperfusion injury is associated with activated mitochondrial autophagy in a mouse model of diabetes". Molecular Medicine Reports 22, no. 3 (2020): 1892-1900. https://doi.org/10.3892/mmr.2020.11270
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