Open Access

Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia

  • Authors:
    • Shanfeng Hao
    • Yang Zhang
    • Luogang Hua
    • Ning Xie
    • Na Xiao
    • Huaquan Wang
    • Rong Fu
    • Zonghong Shao
  • View Affiliations

  • Published online on: June 26, 2020     https://doi.org/10.3892/mmr.2020.11280
  • Pages: 2012-2020
  • Copyright: © Hao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Immuno-related pancytopenia (IRP) is characterized by pancytopenia resulting from bone marrow suppression or destruction mediated by auto‑antibodies. In our previous study, a K562 cDNA library was established, which was used to screen for seven possible auto‑antigens produced by hematopoietic cells in patients with IRP, including ferritin light chain (FTL). In the present study, FTL was expressed and purified, and the levels of the auto‑antibodies specific to FTL were measured. Through ELISA, it was shown that the titer of anti‑FTL antibodies was higher in patients with IRP without treatment compared with those who had recovered from IRP, those with severe aplastic anemia (SAA), those with myelodysplastic syndrome (MDS) and the healthy controls. Furthermore, the expression levels of FTL‑mRNA were upregulated in patients with IRP without treatment compared with those who had recovered from IRP, those with MDS and the normal controls. The results suggest that FTL antibody expression is upregulated in patients with IRP. Detecting FTL antibodies may therefore have certain clinical value in differentiating between IRP, SAA and MDS. Furthermore, in specific patients with IRP, FTL as an auto‑antigen may induce immune attack on hematopoietic stem cells.
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September-2020
Volume 22 Issue 3

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Spandidos Publications style
Hao S, Zhang Y, Hua L, Xie N, Xiao N, Wang H, Fu R and Shao Z: Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia. Mol Med Rep 22: 2012-2020, 2020
APA
Hao, S., Zhang, Y., Hua, L., Xie, N., Xiao, N., Wang, H. ... Shao, Z. (2020). Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia. Molecular Medicine Reports, 22, 2012-2020. https://doi.org/10.3892/mmr.2020.11280
MLA
Hao, S., Zhang, Y., Hua, L., Xie, N., Xiao, N., Wang, H., Fu, R., Shao, Z."Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia". Molecular Medicine Reports 22.3 (2020): 2012-2020.
Chicago
Hao, S., Zhang, Y., Hua, L., Xie, N., Xiao, N., Wang, H., Fu, R., Shao, Z."Antibodies specific to ferritin light chain polypeptide are frequently detected in patients with immune‑related pancytopenia". Molecular Medicine Reports 22, no. 3 (2020): 2012-2020. https://doi.org/10.3892/mmr.2020.11280