Open Access

Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury

  • Authors:
    • Rui Zhang
    • Jiali Wang
    • Baoshan Liu
    • Wenjun Wang
    • Xinhui Fan
    • Boyuan Zheng
    • Qiuhuan Yuan
    • Mengyang Xue
    • Feng Xu
    • Ping Guo
    • Yuguo Chen
  • View Affiliations

  • Published online on: July 6, 2020     https://doi.org/10.3892/mmr.2020.11300
  • Pages: 2487-2495
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑coding RNAs, including long non‑coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs), have significant regulatory effects on a number of biological processes in myocardial ischemia/reperfusion (I/R) injury, including cell differentiation, proliferation and apoptosis. In the present study, the expression levels of lncRNAs, miRNAs and mRNAs were evaluated in a mouse model of myocardial I/R injury. The potential functions of these differentially expressed genes were then analyzed via Gene Ontology and pathway analyses. Additionally, the interactions between lncRNA‑miRNA‑mRNA were predicted by constructing a competing endogenous RNA regulatory network. It was found that 14,366 lncRNAs, 151 miRNAs and 9,377 mRNAs were differentially expressed in mice hearts after I/R compared with the Sham group (fold change >2; P<0.05). The results indicated that these differentially expressed genes were involved in multiple molecular functions, including ‘guanosine diphosphate binding’, ‘RNA polymerase II carboxy‑terminal domain kinase activity’, ‘TATA‑binding protein‑class protein binding’, ‘nicotinamide adenine dinucleotide binding’ and ‘protein phosphatase type 2A regulator activity’. The interactions between lncRNA‑miRNA‑mRNA, including five lncRNAs, 38 miRNAs and 196 mRNAs, were predicted, specifically Gm12040‑mmu‑miR‑125a‑5p‑decapping mRNA 1B, Rpl7l1‑ps1‑mmu‑miR‑124‑3p‑G protein‑coupled receptor 146, Gm11407‑mmu‑miR‑190a‑5p‑homeobox and leucine zipper encoding (HOMEZ), 1600029O15Rik‑mmu‑miR‑132‑3p‑HOMEZ and AK155692‑mmu‑miR‑1224‑3p‑activating transcription factor 6β. Collectively, these findings provided novel insights for future research on lncRNAs, miRNAs and mRNAs in myocardial I/R injury.

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September-2020
Volume 22 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang R, Wang J, Liu B, Wang W, Fan X, Zheng B, Yuan Q, Xue M, Xu F, Guo P, Guo P, et al: Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury. Mol Med Rep 22: 2487-2495, 2020
APA
Zhang, R., Wang, J., Liu, B., Wang, W., Fan, X., Zheng, B. ... Chen, Y. (2020). Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury. Molecular Medicine Reports, 22, 2487-2495. https://doi.org/10.3892/mmr.2020.11300
MLA
Zhang, R., Wang, J., Liu, B., Wang, W., Fan, X., Zheng, B., Yuan, Q., Xue, M., Xu, F., Guo, P., Chen, Y."Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury". Molecular Medicine Reports 22.3 (2020): 2487-2495.
Chicago
Zhang, R., Wang, J., Liu, B., Wang, W., Fan, X., Zheng, B., Yuan, Q., Xue, M., Xu, F., Guo, P., Chen, Y."Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury". Molecular Medicine Reports 22, no. 3 (2020): 2487-2495. https://doi.org/10.3892/mmr.2020.11300