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Article

Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis

  • Authors:
    • Zirui Huang
    • Xiaohong Li
    • Min Qiu
    • Jing Chen
    • Miao Tian
    • Fengzhen Han
    • Yanqiu Ou
    • Xiaoqing Liu
    • Chengbin Zhou
    • Haiyun Yuan
    • Jian Zhuang
    • Jimei Chen
  • View Affiliations / Copyright

    Affiliations: Guangdong Provincial Key Laboratory of South China Structural Heart Disease, Guangdong Cardiovascular Institute, Guangzhou, Guangdong 510080, P.R. China, School of Medicine, South China University of Technology School of Medicine, Guangzhou, Guangdong 510080, P.R. China, Department of Obstetrics and Gynecology, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China, Department of Epidemiology, Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 2528-2536
    |
    Published online on: July 10, 2020
       https://doi.org/10.3892/mmr.2020.11332
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Abstract

Pulmonary stenosis (PS) is a congenital heart disease characterized by a dynamic or fixed anatomic obstruction of blood flow from the right ventricle to the pulmonary arterial vasculature. In the present study, extracellular vesicle long RNAs (EVLRs) from pregnant females who had healthy infants or PS infants were analyzed by RNA sequencing, and their diagnostic potential for PS during pregnancy was evaluated. A method for the selection of genes that could be considered as informative for the prediction PS based on extracellular vesicles (EVs) from pregnant females using long‑read RNA sequencing was developed. Blood samples were collected from females carrying fetuses with PS and females carrying unaffected fetuses (n=6 in each group). Physical characterization of EVs was performed using nanoparticle tracking analysis, transmission electron microscopy and western blotting. EVLRs from plasma were profiled by RNA sequencing and mRNA co‑expression modules were constructed by weighted gene co‑expression network analysis (WGCNA). Gene Ontology (GO) enrichment analysis was used to predict the function of the genes in each module. Hub genes were filtered out based on WGCNA and visualized using Cytoscape. EVLRs consisted of mRNAs, microRNAs and long non‑coding RNA. Overall, 26 modules were identified containing 16,394 genes. All modules were independent of each other. One particular module, referred to as the blue module, was markedly different between the two groups. A total of 735 hub genes in the blue module were identified, of which 33 were visualized, demonstrating the connection between these hub genes. GO enrichment analysis demonstrated that the analyzed hub genes were enriched in ‘glucose transport’, ‘ATP‑dependent chromatin remodeling’, ‘histone deacetylation’, ‘histone H3‑K4 methylation’, ‘DNA methylation’, ‘apoptotic signaling pathway’ and ‘glucocorticoid receptor signaling pathway’. The hub genes identified in this module may provide a genetic framework for prenatal PS diagnosis. Furthermore, functional analysis of these associated genes may provide a theoretical basis for further research on PS pathogenesis.
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1 

Liu Y, Chen S, Zühlke L, Black GC, Choy MK, Li N and Keavney BD: Global birth prevalence of congenital heart defects 1970–2017: Updated systematic review and meta-analysis of 260 studies. Int J Epidemiol. 48:455–463. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Cuypers JA, Witsenburg M, van der Linde D and Roos-Hesselink JW: Pulmonary stenosis: Update on diagnosis and therapeutic options. Heart. 99:339–347. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Patel AB, Ratnayaka K and Bergersen L: A Review: Percutaneous pulmonary artery stenosis therapy: state-of-the-art and look to the future. Cardiol Young. 29:93–99. 2019. View Article : Google Scholar : PubMed/NCBI

4 

Webber DM, MacLeod SL, Bamshad MJ, Shaw GM, Finnell RH, Shete SS, Witte JS, Erickson SW, Murphy LD and Hobbs C: Developments in our understanding of the genetic basis of birth defects. Birth Defects Res A Clin Mol Teratol. 103:680–691. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Bahado-Singh RO, Zaffra R, Albayarak S, Chelliah A, Bolinjkar R, Turkoglu O and Radhakrishna U: Epigenetic markers for newborn congenital heart defect (CHD). J Matern Fetal Neonatal Med. 29:1881–1887. 2016.PubMed/NCBI

6 

van Niel G, DAngelo G and Raposo G: Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 19:213–228. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Shah R, Patel T and Freedman JE: Circulating extracellular vesicles in human disease. N Engl J Med. 379:958–966. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Tong M, Kleffmann T, Pradhan S, Johansson CL, DeSousa J, Stone PR, James JL, Chen Q and Chamley LW: Proteomic characterization of macro-, micro- and nano-extracellular vesicles derived from the same first trimester placenta: Relevance for feto-maternal communication. Hum Reprod. 31:687–699. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Adam S, Elfeky O, Kinhal V, Dutta S, Lai A, Jayabalan N, Nuzhat Z, Palma C, Rice GE and Salomon C: Review: Fetal-maternal communication via extracellular vesicles - Implications for complications of pregnancies. Placenta. 54:83–88. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Colombo M, Raposo G and Théry C: Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol. 30:255–289. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Jin J and Menon R: Placental exosomes: A proxy to understand pregnancy complications. Am J Reprod Immunol. 79:e127882018. View Article : Google Scholar : PubMed/NCBI

12 

Zhou R, Chen KK, Zhang J, Xiao B, Huang Z, Ju C, Sun J, Zhang F, Lv XB and Huang G: The decade of exosomal long RNA species: An emerging cancer antagonist. Mol Cancer. 17:752018. View Article : Google Scholar : PubMed/NCBI

13 

Del Re M, Biasco E, Crucitta S, Derosa L, Rofi E, Orlandini C, Miccoli M, Galli L, Falcone A, Jenster GW, et al: The detection of androgen receptor splice variant 7 in plasma-derived exosomal RNA strongly predicts resistance to hormonal therapy in metastatic prostate cancer patients. Eur Urol. 71:680–687. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Li Y, Zhao J, Yu S, Wang Z, He X, Su Y, Guo T, Sheng H, Chen J, Zheng Q, et al: Extracellular vesicles long RNA sequencing reveals abundant mRNA, circRNA, and lncRNA in human blood as potential biomarkers for cancer diagnosis. Clin Chem. 65:798–808. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Bolger AM, Lohse M and Usadel B: Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics. 30:2114–2120. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Kim D, Langmead B and Salzberg SL: HISAT: A fast spliced aligner with low memory requirements. Nat Methods. 12:357–360. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Langfelder P, Horvath S and Fast R: Fast R functions for robust correlations and hierarchical clustering. J Stat Softw. 46:i112012. View Article : Google Scholar : PubMed/NCBI

18 

Langfelder P and Horvath S: WGCNA: An R package for weighted correlation network analysis. BMC Bioinformatics. 9:5592008. View Article : Google Scholar : PubMed/NCBI

19 

Shi Z, Derow CK and Zhang B: Co-expression module analysis reveals biological processes, genomic gain, and regulatory mechanisms associated with breast cancer progression. BMC Syst Biol. 4:742010. View Article : Google Scholar : PubMed/NCBI

20 

Liu X, Hu AX, Zhao JL and Chen FL: Identification of key gene modules in human osteosarcoma by co-expression analysis weighted gene co-expression network analysis (WGCNA). J Cell Biochem. 118:3953–3959. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Deng J, Kong W, Mou X, Wang S and Zeng W: Identifying novel candidate biomarkers of RCC based on WGCNA analysis. Per Med. 15:381–394. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Maertens A, Tran V, Kleensang A and Hartung T: Weighted Gene correlation network analysis (WGCNA) Reveals novel transcription factors associated with Bisphenol a dose-response. Front Genet. 9:5082018. View Article : Google Scholar : PubMed/NCBI

23 

Xi X, Chu Y, Liu N, Wang Q, Yin Z, Lu Y and Chen Y: Joint bioinformatics analysis of underlying potential functions of hsa-let-7b-5p and core genes in human glioma. J Transl Med. 17:1292019. View Article : Google Scholar : PubMed/NCBI

24 

Cypryk K, Bartyzel L, Zurawska-Klis M, Mlynarski W, Szadkowska A, Wilczynski J, Nowakowska D, Wozniak LA and Fendler W: Continuous glucose monitoring in type 1 diabetes pregnancy shows that fetal heart rate correlates with maternal glycemia. Diabetes Technol Ther. 17:619–624. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Øyen N, Diaz LJ, Leirgul E, Boyd HA, Priest J, Mathiesen ER, Quertermous T, Wohlfahrt J and Melbye M: Prepregnancy diabetes and offspring risk of congenital heart disease: A nationwide cohort study. Circulation. 133:2243–2253. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Hoang TT, Marengo LK, Mitchell LE, Canfield MA and Agopian AJ: Original findings and updated meta-analysis for the association between maternal diabetes and risk for congenital heart disease phenotypes. Am J Epidemiol. 186:118–128. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Helle EIT, Biegley P, Knowles JW, Leader JB, Pendergrass S, Yang W, Reaven GR, Shaw GM, Ritchie M and Priest JR: First trimester plasma glucose values in women without diabetes are associated with risk for congenital heart disease in offspring. J Pediatr. 195:275–278. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Priest JR, Yang W, Reaven G, Knowles JW and Shaw GM: Maternal Midpregnancy glucose levels and risk of congenital heart disease in offspring. JAMA Pediatr. 169:1112–1116. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Moore-Morris T, van Vliet PP, Andelfinger G and Puceat M: Role of epigenetics in cardiac development and congenital diseases. Physiol Rev. 98:2453–2475. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Radhakrishna U, Albayrak S, Zafra R, Baraa A, Vishweswaraiah S, Veerappa AM, Mahishi D, Saiyed N, Mishra NK, Guda C, et al: Placental epigenetics for evaluation of fetal congenital heart defects: Ventricular Septal Defect (VSD). PLoS One. 14:e02002292019. View Article : Google Scholar : PubMed/NCBI

31 

Muntean I, Togănel R and Benedek T: Genetics of congenital heart disease: Past and Present. Biochem Genet. 55:105–123. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Song R, Hu XQ and Zhang L: Glucocorticoids and programming of the microenvironment in heart. J Endocrinol. 242:T121–T133. 2019. View Article : Google Scholar : PubMed/NCBI

33 

Chen T, Li SJ, Chen B, Huang Q, Kong XY, Shen C, Gu HT and Wang XW: Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease. Int J Mol Med. 43:980–992. 2019.PubMed/NCBI

34 

Fowden AL and Forhead AJ: Glucocorticoids as regulatory signals during intrauterine development. Exp Physiol. 100:1477–1487. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Weikum ER, Knuesel MT, Ortlund EA and Yamamoto KR: Glucocorticoid receptor control of transcription: Precision and plasticity via allostery. Nat Rev Mol Cell Biol. 18:159–174. 2017. View Article : Google Scholar : PubMed/NCBI

36 

Rog-Zielinska EA, Thomson A, Kenyon CJ, Brownstein DG, Moran CM, Szumska D, Michailidou Z, Richardson J, Owen E, Watt A, et al: Glucocorticoid receptor is required for foetal heart maturation. Hum Mol Genet. 22:3269–3282. 2013. View Article : Google Scholar : PubMed/NCBI

37 

Rog-Zielinska EA, Craig MA, Manning JR, Richardson RV, Gowans GJ, Dunbar DR, Gharbi K, Kenyon CJ, Holmes MC, Hardie DG, et al: Glucocorticoids promote structural and functional maturation of foetal cardiomyocytes: A role for PGC-1α. Cell Death Differ. 22:1106–1116. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Oakley RH and Cidlowski JA: Glucocorticoid signaling in the heart: A cardiomyocyte perspective. J Steroid Biochem Mol Biol. 153:27–34. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Martinez SR, Ma Q, Dasgupta C, Meng X and Zhang L: MicroRNA-210 suppresses glucocorticoid receptor expression in response to hypoxia in fetal rat cardiomyocytes. Oncotarget. 8:80249–80264. 2017. View Article : Google Scholar : PubMed/NCBI

40 

Ngo TTM, Moufarrej MN, Rasmussen MH, Camunas-Soler J, Pan W, Okamoto J, Neff NF, Liu K, Wong RJ, Downes K, et al: Noninvasive blood tests for fetal development predict gestational age and preterm delivery. Science. 360:1133–1136. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang Z, Li X, Qiu M, Chen J, Tian M, Han F, Ou Y, Liu X, Zhou C, Yuan H, Yuan H, et al: Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis. Mol Med Rep 22: 2528-2536, 2020.
APA
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F. ... Chen, J. (2020). Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis. Molecular Medicine Reports, 22, 2528-2536. https://doi.org/10.3892/mmr.2020.11332
MLA
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F., Ou, Y., Liu, X., Zhou, C., Yuan, H., Zhuang, J., Chen, J."Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis". Molecular Medicine Reports 22.3 (2020): 2528-2536.
Chicago
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F., Ou, Y., Liu, X., Zhou, C., Yuan, H., Zhuang, J., Chen, J."Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis". Molecular Medicine Reports 22, no. 3 (2020): 2528-2536. https://doi.org/10.3892/mmr.2020.11332
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Z, Li X, Qiu M, Chen J, Tian M, Han F, Ou Y, Liu X, Zhou C, Yuan H, Yuan H, et al: Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis. Mol Med Rep 22: 2528-2536, 2020.
APA
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F. ... Chen, J. (2020). Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis. Molecular Medicine Reports, 22, 2528-2536. https://doi.org/10.3892/mmr.2020.11332
MLA
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F., Ou, Y., Liu, X., Zhou, C., Yuan, H., Zhuang, J., Chen, J."Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis". Molecular Medicine Reports 22.3 (2020): 2528-2536.
Chicago
Huang, Z., Li, X., Qiu, M., Chen, J., Tian, M., Han, F., Ou, Y., Liu, X., Zhou, C., Yuan, H., Zhuang, J., Chen, J."Weighted gene co‑expression network analysis identifies key genes from extracellular vesicles as potential prognostic biomarkers for congenital pulmonary stenosis". Molecular Medicine Reports 22, no. 3 (2020): 2528-2536. https://doi.org/10.3892/mmr.2020.11332
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