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Article Open Access

Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome

  • Authors:
    • Tingqian Ming
    • Min Yuan
    • Qian Kong
    • Qin Huang
    • Zhongyuan Xia
    • Xiaojing Wu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Ming et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2507-2515
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    Published online on: July 10, 2020
       https://doi.org/10.3892/mmr.2020.11335
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Abstract

Blunt chest trauma with hemorrhagic shock frequently induces pulmonary inflammation that leads to acute lung injury (ALI). The present study aimed to explore the protective effects of dexmedetomidine (Dex) in blunt chest trauma and hemorrhagic shock‑resuscitation (THSR)‑induced ALI by mediating nucleotide binding and oligomerization domain‑like receptor family pyrin domain‑containing protein 3 (NLRP3) inflammasome formation in rats. An ALI model in rats induced by THSR was constructed and Dex was administered intraperitoneally (5 µg/kg/h) immediately after blunt chest trauma. Blood samples were collected for the determination of proinflammatory factor levels, and lung tissue specimens were harvested for wet/dry (W/D) weight ratio, hematoxylin and eosin staining, and transmission electron microscopy analyses. Additionally, malondialdehyde (MDA), superoxide dismutase (SOD), lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activity were evaluated, and the expression of protein in lung tissues was examined via western blot analysis. Compared with the sham group, pathological alterations in the ALI group and the W/D ratios were significantly increased. MDA, LDH and MPO activity, and the levels of interleukin (IL)‑1β, IL‑18, IL‑6 and tumor necrosis factor‑α were significantly elevated. NLRP3, apoptosis‑associated speck‑like protein containing a caspase recruitment domain and caspase‑1 expression was significantly increased. Conversely, Dex treatment significantly reversed these changes. The present study demonstrated that by reducing inflammatory responses, Dex exerted protective effects against THSR‑ALI in rats, potentially via the inhibition of NLRP3 signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Ming T, Yuan M, Kong Q, Huang Q, Xia Z and Wu X: Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome. Mol Med Rep 22: 2507-2515, 2020.
APA
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., & Wu, X. (2020). Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome. Molecular Medicine Reports, 22, 2507-2515. https://doi.org/10.3892/mmr.2020.11335
MLA
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., Wu, X."Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome". Molecular Medicine Reports 22.3 (2020): 2507-2515.
Chicago
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., Wu, X."Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome". Molecular Medicine Reports 22, no. 3 (2020): 2507-2515. https://doi.org/10.3892/mmr.2020.11335
Copy and paste a formatted citation
x
Spandidos Publications style
Ming T, Yuan M, Kong Q, Huang Q, Xia Z and Wu X: Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome. Mol Med Rep 22: 2507-2515, 2020.
APA
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., & Wu, X. (2020). Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome. Molecular Medicine Reports, 22, 2507-2515. https://doi.org/10.3892/mmr.2020.11335
MLA
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., Wu, X."Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome". Molecular Medicine Reports 22.3 (2020): 2507-2515.
Chicago
Ming, T., Yuan, M., Kong, Q., Huang, Q., Xia, Z., Wu, X."Dexmedetomidine alleviates blunt chest trauma and hemorrhagic shock‑resuscitation‑induced acute lung injury through inhibiting the NLRP3 inflammasome". Molecular Medicine Reports 22, no. 3 (2020): 2507-2515. https://doi.org/10.3892/mmr.2020.11335
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