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Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells

  • Authors:
    • Soohyun Hwang
    • Hyemin Seong
    • Jinhyun Ryu
    • Joo Yeon Jeong
    • Tae Seen Kang
    • Ki Yup Nam
    • Seong Wook Seo
    • Seong Jae Kim
    • Sang Soo Kang
    • Yong Seop Han
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Convergence Medical Science, College of Medicine, Gyeongsang National University, Jinju, South Gyeongsang 52727, Republic of Korea, Department of Ophthalmology, Gyeongsang National University Changwon Hospital, Changwon, Gyeongsangnam‑do 51472, Republic of Korea, Department of Ophthalmology, College of Medicine, Gyeongsang National University, Jinju, South Gyeongsang 52727, Republic of Korea
    Copyright: © Hwang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2733-2740
    |
    Published online on: July 16, 2020
       https://doi.org/10.3892/mmr.2020.11344
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Abstract

Neovascularization in the retina can cause loss of vision. Vascular endothelial growth factor (VEGF) serves an important role in the pathogenesis of retinal vascular diseases. Hypoxia is a notable cause of VEGF release and both STAT3 and ERBB2 are known to be associated with VEGF. In addition, STAT3 and ERBB2 interact with each other. In the present study, it was hypothesized that signal transducer and activator of transcription 3 (STAT3) and erbB‑2 receptor tyrosine kinase 2 (ERBB2) may be involved in the regulation of hypoxia‑induced VEGF in the retina. Cells of the retinal pigment epithelium (RPE) are an important source of VEGF. Therefore, the RPE‑derived human cell line ARPE‑19 was exposed to hypoxia. Hypoxia‑induced phosphorylation of STAT3 and ERBB2 in ARPE‑19 cells was decreased by AG490, an inhibitor of Janus kinase 2, as were hypoxia‑induced VEGF release and tube formation in human umbilical vein endothelial cells. Thus, phosphorylation of ERBB2 and STAT3 regulates hypoxia‑induced VEGF release in ARPE‑19 cells. The results of the present study suggested that inhibition of ERBB2 and STAT3‑mediated pathways under hypoxia may represent a new strategy for treating retinal vascular disease.
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Copy and paste a formatted citation
Spandidos Publications style
Hwang S, Seong H, Ryu J, Jeong JY, Kang TS, Nam KY, Seo SW, Kim SJ, Kang SS, Han YS, Han YS, et al: Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells. Mol Med Rep 22: 2733-2740, 2020.
APA
Hwang, S., Seong, H., Ryu, J., Jeong, J.Y., Kang, T.S., Nam, K.Y. ... Han, Y.S. (2020). Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells. Molecular Medicine Reports, 22, 2733-2740. https://doi.org/10.3892/mmr.2020.11344
MLA
Hwang, S., Seong, H., Ryu, J., Jeong, J. Y., Kang, T. S., Nam, K. Y., Seo, S. W., Kim, S. J., Kang, S. S., Han, Y. S."Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells". Molecular Medicine Reports 22.4 (2020): 2733-2740.
Chicago
Hwang, S., Seong, H., Ryu, J., Jeong, J. Y., Kang, T. S., Nam, K. Y., Seo, S. W., Kim, S. J., Kang, S. S., Han, Y. S."Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells". Molecular Medicine Reports 22, no. 4 (2020): 2733-2740. https://doi.org/10.3892/mmr.2020.11344
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang S, Seong H, Ryu J, Jeong JY, Kang TS, Nam KY, Seo SW, Kim SJ, Kang SS, Han YS, Han YS, et al: Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells. Mol Med Rep 22: 2733-2740, 2020.
APA
Hwang, S., Seong, H., Ryu, J., Jeong, J.Y., Kang, T.S., Nam, K.Y. ... Han, Y.S. (2020). Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells. Molecular Medicine Reports, 22, 2733-2740. https://doi.org/10.3892/mmr.2020.11344
MLA
Hwang, S., Seong, H., Ryu, J., Jeong, J. Y., Kang, T. S., Nam, K. Y., Seo, S. W., Kim, S. J., Kang, S. S., Han, Y. S."Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells". Molecular Medicine Reports 22.4 (2020): 2733-2740.
Chicago
Hwang, S., Seong, H., Ryu, J., Jeong, J. Y., Kang, T. S., Nam, K. Y., Seo, S. W., Kim, S. J., Kang, S. S., Han, Y. S."Phosphorylation of STAT3 and ERBB2 mediates hypoxia‑induced VEGF release in ARPE‑19 cells". Molecular Medicine Reports 22, no. 4 (2020): 2733-2740. https://doi.org/10.3892/mmr.2020.11344
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