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Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein

  • Authors:
    • Cai‑Ping Zhang
    • Xin‑Xin Ding
    • Tian Tian
    • Bo‑Jie Li
    • Chu‑Yao Wang
    • Su‑Su Jiang
    • Jin‑Qi Shao
    • Yu‑Lin Yuan
    • Ying Tian
    • Min Zhang
    • Shi‑Yin Long
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Medical College, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Clinical Laboratory, The First Hospital of Changsha, Changsha, Hunan 410005, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2665-2672
    |
    Published online on: July 16, 2020
       https://doi.org/10.3892/mmr.2020.11345
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Abstract

Oxidative stress induces the formation of oxidized low‑density lipoprotein (ox‑LDL), which accelerates the development of atherosclerosis and the rupture of atherosclerotic plaques by promoting lipid accumulation and inhibiting autophagy in vascular cells. Lipophagy is known to be involved in maintaining the balance of neutral lipid metabolism; however, the phenomenon of lipophagy deficiency in ox‑LDL‑treated endothelial cells (ECs) remains to be elucidated. It has been demonstrated that lipid accumulation caused by ox‑LDL inhibits autophagy, which promotes apoptosis in ECs. The aim of the present study was to investigate the association between decreased autophagy and lipid accumulation in ECs treated with ox‑LDL. Electron microscopy demonstrated that the formation of autolipophagosomes was decreased in ox‑LDL‑treated human umbilical vein ECs compared with that in the LDL‑treated group and was accompanied by a decrease in the autophagy‑associated proteins via western blotting analysis. Using laser focal colocalization detection, decreased lipid processing was observed in the lysosomes of ox‑LDL‑treated ECs, which indicated that lipophagy may be attenuated and subsequently result in lipid accumulation in ox‑LDL‑treated ECs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang CP, Ding XX, Tian T, Li BJ, Wang CY, Jiang SS, Shao JQ, Yuan YL, Tian Y, Zhang M, Zhang M, et al: Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Mol Med Rep 22: 2665-2672, 2020.
APA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S. ... Long, S. (2020). Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Molecular Medicine Reports, 22, 2665-2672. https://doi.org/10.3892/mmr.2020.11345
MLA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22.4 (2020): 2665-2672.
Chicago
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22, no. 4 (2020): 2665-2672. https://doi.org/10.3892/mmr.2020.11345
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang CP, Ding XX, Tian T, Li BJ, Wang CY, Jiang SS, Shao JQ, Yuan YL, Tian Y, Zhang M, Zhang M, et al: Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Mol Med Rep 22: 2665-2672, 2020.
APA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S. ... Long, S. (2020). Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Molecular Medicine Reports, 22, 2665-2672. https://doi.org/10.3892/mmr.2020.11345
MLA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22.4 (2020): 2665-2672.
Chicago
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22, no. 4 (2020): 2665-2672. https://doi.org/10.3892/mmr.2020.11345
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