Open Access

Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein

  • Authors:
    • Cai‑Ping Zhang
    • Xin‑Xin Ding
    • Tian Tian
    • Bo‑Jie Li
    • Chu‑Yao Wang
    • Su‑Su Jiang
    • Jin‑Qi Shao
    • Yu‑Lin Yuan
    • Ying Tian
    • Min Zhang
    • Shi‑Yin Long
  • View Affiliations

  • Published online on: July 16, 2020     https://doi.org/10.3892/mmr.2020.11345
  • Pages: 2665-2672
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Oxidative stress induces the formation of oxidized low‑density lipoprotein (ox‑LDL), which accelerates the development of atherosclerosis and the rupture of atherosclerotic plaques by promoting lipid accumulation and inhibiting autophagy in vascular cells. Lipophagy is known to be involved in maintaining the balance of neutral lipid metabolism; however, the phenomenon of lipophagy deficiency in ox‑LDL‑treated endothelial cells (ECs) remains to be elucidated. It has been demonstrated that lipid accumulation caused by ox‑LDL inhibits autophagy, which promotes apoptosis in ECs. The aim of the present study was to investigate the association between decreased autophagy and lipid accumulation in ECs treated with ox‑LDL. Electron microscopy demonstrated that the formation of autolipophagosomes was decreased in ox‑LDL‑treated human umbilical vein ECs compared with that in the LDL‑treated group and was accompanied by a decrease in the autophagy‑associated proteins via western blotting analysis. Using laser focal colocalization detection, decreased lipid processing was observed in the lysosomes of ox‑LDL‑treated ECs, which indicated that lipophagy may be attenuated and subsequently result in lipid accumulation in ox‑LDL‑treated ECs.
View Figures
View References

Related Articles

Journal Cover

October-2020
Volume 22 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang CP, Ding XX, Tian T, Li BJ, Wang CY, Jiang SS, Shao JQ, Yuan YL, Tian Y, Zhang M, Zhang M, et al: Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Mol Med Rep 22: 2665-2672, 2020
APA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S. ... Long, S. (2020). Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein. Molecular Medicine Reports, 22, 2665-2672. https://doi.org/10.3892/mmr.2020.11345
MLA
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22.4 (2020): 2665-2672.
Chicago
Zhang, C., Ding, X., Tian, T., Li, B., Wang, C., Jiang, S., Shao, J., Yuan, Y., Tian, Y., Zhang, M., Long, S."Impaired lipophagy in endothelial cells with prolonged exposure to oxidized low‑density lipoprotein". Molecular Medicine Reports 22, no. 4 (2020): 2665-2672. https://doi.org/10.3892/mmr.2020.11345