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Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes

  • Authors:
    • Jinsheng Chen
    • Xiyun Bian
    • Yanxia Li
    • Xiaolin Xiao
    • Yanying Yin
    • Xinping Du
    • Cuancuan Wang
    • Lili Li
    • Yaowu Bai
    • Xiaozhi Liu
  • View Affiliations / Copyright

    Affiliations: North China University of Science and Technology, Tangshan, Hebei 063210, P.R. China, Central Laboratory, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Department of Neurology, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Department of Cardiology, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China, Department of Bone and Soft Tissue Tumors, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2617-2626
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    Published online on: July 28, 2020
       https://doi.org/10.3892/mmr.2020.11374
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Abstract

Moderate hypothermia plays a major role in myocardial cell death as a result of hypoxia/reoxygenation (H/R) injury. However, few studies have investigated the molecular mechanisms of hypothermic cardioprotection. Several responses to stress and other cell functions are regulated by post‑translational protein modifications controlled by small ubiquitin‑like modifier (SUMO). Previous studies have established that high SUMOylation of proteins potentiates the ability of cells to withstand hypoxic‑ischemic stress. The level to which moderate hypothermia affects SUMOylation is not fully understood, as the functions of SUMOylation in the heart have not been studied in depth. The aim of the present study was to investigate the effect of moderate hypothermia (33˚C) on the protective functions of SUMOylation on myocardial cells. HL‑1 and H9c2 cells were treated with the hypoxia‑mimetic chemical CoCl2 and complete medium to simulate H/R injury. Hypothermia intervention was then administered. A Cell Counting kit‑8 assay was used to analyze cell viability. Mitochondrial membrane potential and the generation of reactive oxygen species (ROS) were used as functional indexes of mitochondria dysfunction. Bcl‑2 and caspase‑3 expression levels were analyzed by western blotting. The present results suggested that moderate hypothermia significantly increased SUMO1 and Bcl‑2 expression levels, as well as the mitochondrial membrane potential, but significantly decreased the expression levels of caspase‑3 and mitochondrial ROS. Thus, moderate hypothermia may enhance SUMOylation and attenuate myocardial H/R injury. Moreover, a combination of SUMOylation and moderate hypothermia may be a potential cardiovascular intervention.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Bian X, Li Y, Xiao X, Yin Y, Du X, Wang C, Li L, Bai Y, Liu X, Liu X, et al: Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes. Mol Med Rep 22: 2617-2626, 2020.
APA
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X. ... Liu, X. (2020). Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes. Molecular Medicine Reports, 22, 2617-2626. https://doi.org/10.3892/mmr.2020.11374
MLA
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X., Wang, C., Li, L., Bai, Y., Liu, X."Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes". Molecular Medicine Reports 22.4 (2020): 2617-2626.
Chicago
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X., Wang, C., Li, L., Bai, Y., Liu, X."Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes". Molecular Medicine Reports 22, no. 4 (2020): 2617-2626. https://doi.org/10.3892/mmr.2020.11374
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Bian X, Li Y, Xiao X, Yin Y, Du X, Wang C, Li L, Bai Y, Liu X, Liu X, et al: Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes. Mol Med Rep 22: 2617-2626, 2020.
APA
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X. ... Liu, X. (2020). Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes. Molecular Medicine Reports, 22, 2617-2626. https://doi.org/10.3892/mmr.2020.11374
MLA
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X., Wang, C., Li, L., Bai, Y., Liu, X."Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes". Molecular Medicine Reports 22.4 (2020): 2617-2626.
Chicago
Chen, J., Bian, X., Li, Y., Xiao, X., Yin, Y., Du, X., Wang, C., Li, L., Bai, Y., Liu, X."Moderate hypothermia induces protection against hypoxia/reoxygenation injury by enhancing SUMOylation in cardiomyocytes". Molecular Medicine Reports 22, no. 4 (2020): 2617-2626. https://doi.org/10.3892/mmr.2020.11374
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