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IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway

  • Authors:
    • Qianya Su
    • Fei Wang
    • Zhengbang Dong
    • Mei Chen
    • Rong Cao
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, School of Medicine, Zhong Da Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China
    Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3111-3116
    |
    Published online on: August 3, 2020
       https://doi.org/10.3892/mmr.2020.11403
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Abstract

The present study aimed to investigate the role of janus kinase (JAK)1/STAT1 in interferon (IFN)‑γ‑induced apoptosis in human melanocytes. Following IFN‑γ treatment, the viability of human melanocytes were analyzed using a Cell Counting Kit‑8 assay and the apoptotic rate was determined using flow cytometry. Western blotting was also performed to analyze the phosphorylation levels of JAK1, JAK2 and the transcriptional factor STAT1, as well as the expression levels of Bcl‑2, Bax, Bcl‑2 homologous antagonist killer (Bak) and cleaved caspase‑3. Finally, following the pretreatment with the STAT1 inhibitor fludarabine, human melanocytes were treated with IFN‑γ and flow cytometry was used to detect the apoptotic rate. The results revealed that IFN‑γ reduced the proliferation and induced the apoptosis of human melanocytes. In addition, IFN‑γ treatment led to decreased expression levels of Bcl‑2 and increased expression levels of Bax, Bak and cleaved caspase‑3, alongside the activation of the JAK1/STAT1 signaling pathway. Conversely, the pretreatment with the STAT1 inhibitor fludarabine decreased the apoptotic rate of human melanocytes following IFN‑γ induction. In conclusion, the findings of the present study suggested that IFN‑γ may induce the apoptosis of human melanocytes by activating the JAK1/STAT1 signaling pathway, alongside increasing the expression levels of Bax, Bak and cleaved caspase‑3, and decreasing the expression levels of Bcl‑2.
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1 

Taieb A, Alomar A, Böhm M, Dell'anna ML, De Pase A, Eleftheriadou V, Ezzedine K, Gauthier Y, Gawkrodger DJ, Jouary T, et al Vitiligo European Task Force (VETF); European Academy of Dermatology: Venereology (EADV); Union Europe´enne des Me´decins Spe´cialistes (UEMS), : Guidelines for the management of vitiligo: The European Dermatology Forum consensus. Br J Dermatol. 168:3111–19. 2013. View Article : Google Scholar

2 

Manga P, Elbuluk N and Orlow SJ: Recent advances in understanding vitiligo. F1000 Res. 5:52016. View Article : Google Scholar

3 

Arya V, Bansal M, Girard L, Arya S and Valluri A: Vitiligo at Injection Site of PEG-IFN-α 2a in Two Patients with Chronic Hepatitis C: Case Report and Literature Review. Case Rep Dermatol. 2:156–164. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Hamadah I, Binamer Y, Sanai FM, Abdo AA and Alajlan A: Interferon-induced vitiligo in hepatitis C patients: A case series. Int J Dermatol. 49:829–833. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Rozera C, Cappellini GA, D'Agostino G, Santodonato L, Castiello L, Urbani F, Macchia I, Aricò E, Casorelli I, Sestili P, et al: Intratumoral injection of IFN-alpha dendritic cells after dacarbazine activates anti-tumor immunity: Results from a phase I trial in advanced melanoma. J Transl Med. 13:1392015. View Article : Google Scholar : PubMed/NCBI

6 

Wang S, Liu D, Ning W and Xu A: Cytosolic dsDNA triggers apoptosis and pro-inflammatory cytokine production in normal human melanocytes. Exp Dermatol. 24:298–300. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Harris JE, Harris TH, Weninger W, Wherry EJ, Hunter CA and Turka LA: A mouse model of vitiligo with focused epidermal depigmentation requires IFN-γ for autoreactive CD8+ T-cell accumulation in the skin. J Invest Dermatol. 132:1869–1876. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Rashighi M and Harris JE: Interfering with the IFN-γ/CXCL10 pathway to develop new targeted treatments for vitiligo. Ann Transl Med. 3:3432015.PubMed/NCBI

9 

Yang L, Wei Y, Sun Y, Shi W, Yang J, Zhu L and Li M: Interferon-gamma Inhibits Melanogenesis and Induces Apoptosis in Melanocytes: A Pivotal Role of CD8+ Cytotoxic T lymphocytes in Vitiligo. Acta Derm Venereol. 95:664–670. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Lee JH, Kwon HS, Jung HM, Lee H, Kim GM, Yim HW and Bae JM: Treatment Outcomes of Topical Calcineurin Inhibitor Therapy for Patients With Vitiligo: A Systematic Review and Meta-analysis. JAMA Dermatol. 155:9292019. View Article : Google Scholar

11 

Nahhas AF, Mohammad TF and Hamzavi IH: Vitiligo Surgery: Shuffling Melanocytes. J Investig Dermatol Symp Proc. 18:S34–S37. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Ma J, Li S, Zhu L, Guo S, Yi X, Cui T, He Y, Chang Y, Liu B, Li C, et al: Baicalein protects human vitiligo melanocytes from oxidative stress through activation of NF-E2-related factor2 (Nrf2) signaling pathway. Free Radic Biol Med. 129:492–503. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Yang K, Xiong X, Pallavi G, Ling Y, Ding F, Duan W, Sun W, Ding G, Gong Q, Zhu W, et al: The early repigmentation pattern of vitiligo is related to the source of melanocytes and by the choice of therapy: A retrospective cohort study. Int J Dermatol. 57:324–331. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Jia H, Song L, Cong Q, Wang J, Xu H, Chu Y, Li Q, Zhang Y, Zou X, Zhang C, et al: The LIM protein AJUBA promotes colorectal cancer cell survival through suppression of JAK1/STAT1/IFIT2 network. Oncogene. 36:2655–2666. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Chou DH, Vetere A, Choudhary A, Scully SS, Schenone M, Tang A, Gomez R, Burns SM, Lundh M, Vital T, et al: Kinase-Independent Small-Molecule Inhibition of JAK-STAT Signaling. J Am Chem Soc. 137:7929–7934. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Kaplan DH, Greenlund AC, Tanner JW, Shaw AS and Schreiber RD: Identification of an interferon-gamma receptor alpha chain sequence required for JAK-1 binding. J Biol Chem. 271:9–12. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Schroder K, Hertzog PJ, Ravasi T and Hume DA: Interferon-gamma: An overview of signals, mechanisms and functions. J Leukoc Biol. 75:163–189. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Strassner JP and Harris JE: Understanding mechanisms of autoimmunity through translational research in vitiligo. Curr Opin Immunol. 43:81–88. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Richmond JM, Bangari DS, Essien KI, Currimbhoy SD, Groom JR, Pandya AG, Youd ME, Luster AD and Harris JE: Keratinocyte-Derived Chemokines Orchestrate T-Cell Positioning in the Epidermis during Vitiligo and May Serve as Biomarkers of Disease. J Invest Dermatol. 137:350–358. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Lu B, Antoine DJ, Kwan K, Lundbäck P, Wähämaa H, Schierbeck H, Robinson M, Van Zoelen MA, Yang H, Li J, et al: JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. Proc Natl Acad Sci USA. 111:3068–3073. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Ahn J, Lee J and Kim S: Interferon-gamma inhibits the neuronal differentiation of neural progenitor cells by inhibiting the expression of Neurogenin2 via the JAK/STAT1 pathway. Biochem Biophys Res Commun. 466:52–59. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Wang S, Zheng G, Zhao L, Xu F and Qian J: Shp-2 contributes to anti-RSV activity in human pulmonary alveolar epithelial cells by interfering with the IFN-α-induced Jak/Stat1 pathway. J Cell Mol Med. 19:2432–2440. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Li T, Dong ZR, Guo ZY, Wang CH, Tang ZY, Qu SF, Chen ZT, Li XW and Zhi XT: Aspirin enhances IFN-α-induced growth inhibition and apoptosis of hepatocellular carcinoma via JAK1/STAT1 pathway. Cancer Gene Ther. 20:366–374. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Cao ZH, Zheng QY, Li GQ, Hu XB, Feng SL, Xu GL and Zhang KQ: STAT1-mediated down-regulation of Bcl-2 expression is involved in IFN-γ/TNF-α-induced apoptosis in NIT-1 cells. PLoS One. 10:e01209212015. View Article : Google Scholar : PubMed/NCBI

25 

Zhu L, Hao J, Cheng M, Zhang C, Huo C, Liu Y, Du W and Zhang X: Hyperglycemia-induced Bcl-2/Bax-mediated apoptosis of Schwann cells via mTORC1/S6K1 inhibition in diabetic peripheral neuropathy. Exp Cell Res. 367:186–195. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Toscano ECB, Vieira ÉLM, Portela ACDC, Reis JLJ, Caliari MV, Giannetti AV, Gonçalves AP, Siqueira JM, Suemoto CK, Leite REP, et al: Bcl-2/Bax ratio increase does not prevent apoptosis of glia and granular neurons in patients with temporal lobe epilepsy. Neuropathology. 39:348–357. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Zhang J, Xia Y, Xu Z and Deng X: Propofol Suppressed Hypoxia/Reoxygenation-Induced Apoptosis in HBVSMC by Regulation of the Expression of Bcl-2, Bax, Caspase3, Kir6.1, and p-JNK. Oxid Med Cell Longev. 2016:15187382016. View Article : Google Scholar : PubMed/NCBI

28 

Zhao L, Zhu Z, Yao C, Huang Y, Zhi E, Chen H, Tian R, Li P, Yuan Q, Xue Y, et al: VEGFC/VEGFR3 Signaling Regulates Mouse Spermatogonial Cell Proliferation via the Activation of AKT/MAPK and Cyclin D1 Pathway and Mediates the Apoptosis by affecting Caspase 3/9 and Bcl-2. Cell Cycle. 17:225–239. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Rashighi M, Agarwal P, Richmond JM, Harris TH, Dresser K, Su MW, Zhou Y, Deng A, Hunter CA, Luster AD, et al: CXCL10 is critical for the progression and maintenance of depigmentation in a mouse model of vitiligo. Sci Transl Med. 6:223ra232014. View Article : Google Scholar : PubMed/NCBI

30 

Harris JE: IFN-γ in Vitiligo, Is It the Fuel or the Fire? Acta Derm Venereol. 95:643–644. 2015.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Su Q, Wang F, Dong Z, Chen M and Cao R: IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Mol Med Rep 22: 3111-3116, 2020.
APA
Su, Q., Wang, F., Dong, Z., Chen, M., & Cao, R. (2020). IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Molecular Medicine Reports, 22, 3111-3116. https://doi.org/10.3892/mmr.2020.11403
MLA
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22.4 (2020): 3111-3116.
Chicago
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22, no. 4 (2020): 3111-3116. https://doi.org/10.3892/mmr.2020.11403
Copy and paste a formatted citation
x
Spandidos Publications style
Su Q, Wang F, Dong Z, Chen M and Cao R: IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Mol Med Rep 22: 3111-3116, 2020.
APA
Su, Q., Wang, F., Dong, Z., Chen, M., & Cao, R. (2020). IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Molecular Medicine Reports, 22, 3111-3116. https://doi.org/10.3892/mmr.2020.11403
MLA
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22.4 (2020): 3111-3116.
Chicago
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22, no. 4 (2020): 3111-3116. https://doi.org/10.3892/mmr.2020.11403
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