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Article Open Access

Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells

  • Authors:
    • Haoyue Luo
    • Shaobo Cong
    • Jiaojiao Dong
    • Litao Jin
    • Dandan Jiang
    • Xingang Wang
    • Qingfeng Chen
    • Funian Li
  • View Affiliations / Copyright

    Affiliations: Department of Breast Disease Diagnosis, Treatment Centre, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao, Shandong 266021, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3183-3190
    |
    Published online on: August 4, 2020
       https://doi.org/10.3892/mmr.2020.11414
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Abstract

Multidrug resistance (MDR) is a major cause of disease relapse and mortality in breast cancer. Paired‑related homeobox 1 (PRRX1) is associated with the epithelial‑mesenchymal transition (EMT), which is involved in tumor development, including cell invasion and MDR. However, the effect of PRRX1 on MDR had not clearly established. The present study investigated the influence of PRRX1 on MDR and the underlying molecular mechanisms in MCF‑7 breast cancer cells. MCF‑7 cells were divided into PRRX1+ group (cells transfected with a recombinant plasmid carrying the PRRX1 gene), negative control group (cells transfected with a blank vector) and blank group (untreated cells). It was found that the relative protein and mRNA expression levels of PRRX1, N‑cadherin, vimentin and P‑glycoprotein were significantly higher in PRRX1‑overexpressing MCF‑7 cells compared with those in control cells. The half‑maximal inhibitory concentration of three groups after treatment with docetaxel and cis‑platinum complexes were significantly higher in PRRX1‑overexpressing MCF‑7 cells compared with those in control cells. Furthermore, relative PTEN expression decreased significantly and levels of phosphorylated PI3K and AKT increased substantially in PRRX1‑overexpressing MCF‑7 cells. These results indicated that PRRX1 overexpression may induce MDR via PTEN/PI3K/AKT signaling in breast cancer. It is highly recommended that PRRX1 gene expression detection should be performed in patients with breast cancer to aid the selection of more appropriate treatments, which will lead to an improved prognosis in clinical practice.
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Copy and paste a formatted citation
Spandidos Publications style
Luo H, Cong S, Dong J, Jin L, Jiang D, Wang X, Chen Q and Li F: Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells. Mol Med Rep 22: 3183-3190, 2020.
APA
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X. ... Li, F. (2020). Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells. Molecular Medicine Reports, 22, 3183-3190. https://doi.org/10.3892/mmr.2020.11414
MLA
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X., Chen, Q., Li, F."Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells". Molecular Medicine Reports 22.4 (2020): 3183-3190.
Chicago
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X., Chen, Q., Li, F."Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells". Molecular Medicine Reports 22, no. 4 (2020): 3183-3190. https://doi.org/10.3892/mmr.2020.11414
Copy and paste a formatted citation
x
Spandidos Publications style
Luo H, Cong S, Dong J, Jin L, Jiang D, Wang X, Chen Q and Li F: Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells. Mol Med Rep 22: 3183-3190, 2020.
APA
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X. ... Li, F. (2020). Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells. Molecular Medicine Reports, 22, 3183-3190. https://doi.org/10.3892/mmr.2020.11414
MLA
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X., Chen, Q., Li, F."Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells". Molecular Medicine Reports 22.4 (2020): 3183-3190.
Chicago
Luo, H., Cong, S., Dong, J., Jin, L., Jiang, D., Wang, X., Chen, Q., Li, F."Paired‑related homeobox 1 overexpression promotes multidrug resistance via PTEN/PI3K/AKT signaling in MCF‑7 breast cancer cells". Molecular Medicine Reports 22, no. 4 (2020): 3183-3190. https://doi.org/10.3892/mmr.2020.11414
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