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N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway

  • Authors:
    • Xuemei Wang
    • Mengyi Jiang
    • Xiaoping He
    • Bo Zhang
    • Wei Peng
    • Ling Guo
  • View Affiliations / Copyright

    Affiliations: Department of Prosthodontics, Hospital of Stomatology Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3299-3306
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    Published online on: August 13, 2020
       https://doi.org/10.3892/mmr.2020.11433
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Abstract

N-acetyl cysteine (NAC) has been used to inhibit lipopolysaccharide (LPS)-induced inflammation. However, the molecular mechanism underlying its anti‑inflammatory effects remains to be elucidated. The present study aimed to determine the effect of NAC on the LPS‑induced inflammatory response in bone marrow mesenchymal stem cells (BMSCs) and elucidate the underlying molecular mechanism. First, BMSCs were stimulated by LPS following pretreatment with NAC (0, 0.1, 0.5, 1 or 2 mM). A Cell Counting Kit 8 assay was used to determine the number of viable cells and 1 mM NAC was selected as the experimental concentration. Then, the secretion of inflammatory factors, including interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α was evaluated by enzyme‑linked immunosorbent assay. Finally, the expression levels of mRNA and proteins, including apoptosis‑associated speck‑like protein containing a CARD (ASC), nucleotide‑binding oligomerization domain‑like receptor protein 3 (NLRP3), caspase‑1, thioredoxin‑interacting protein (TXNIP), and thioredoxin (TRX), were evaluated by reverse transcription‑quantitative PCR and western blot analysis, respectively. The results demonstrated that the secretion of inflammatory factors, which was increased by the administration of LPS, was reduced by pretreatment with NAC. Furthermore, NAC reduced the expression of ASC, NLRP3, caspase‑1 and TXNIP, but enhanced that of TRX. To conclude, NAC had anti‑inflammatory effects on LPS‑stimulated BMSCs, which was closely associated with the TXNIP/NLRP3/IL‑1β signaling pathway. Thus, NAC may be a promising treatment to attenuate the inflammatory response in LPS‑induced BMSCs.
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Spandidos Publications style
Wang X, Jiang M, He X, Zhang B, Peng W and Guo L: N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway. Mol Med Rep 22: 3299-3306, 2020.
APA
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., & Guo, L. (2020). N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway. Molecular Medicine Reports, 22, 3299-3306. https://doi.org/10.3892/mmr.2020.11433
MLA
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., Guo, L."N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway". Molecular Medicine Reports 22.4 (2020): 3299-3306.
Chicago
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., Guo, L."N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway". Molecular Medicine Reports 22, no. 4 (2020): 3299-3306. https://doi.org/10.3892/mmr.2020.11433
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Spandidos Publications style
Wang X, Jiang M, He X, Zhang B, Peng W and Guo L: N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway. Mol Med Rep 22: 3299-3306, 2020.
APA
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., & Guo, L. (2020). N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway. Molecular Medicine Reports, 22, 3299-3306. https://doi.org/10.3892/mmr.2020.11433
MLA
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., Guo, L."N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway". Molecular Medicine Reports 22.4 (2020): 3299-3306.
Chicago
Wang, X., Jiang, M., He, X., Zhang, B., Peng, W., Guo, L."N‑acetyl cysteine inhibits the lipopolysaccharide‑induced inflammatory response in bone marrow mesenchymal stem cells by suppressing the TXNIP/NLRP3/IL‑1β signaling pathway". Molecular Medicine Reports 22, no. 4 (2020): 3299-3306. https://doi.org/10.3892/mmr.2020.11433
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