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lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells

  • Authors:
    • Jie Xiang
    • Hai‑Qing Fu
    • Zhun Xu
    • Wei‑Jie Fan
    • Fei Liu
    • Bin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Orthopedics and Traumatology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3715-3722
    |
    Published online on: September 2, 2020
       https://doi.org/10.3892/mmr.2020.11489
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Abstract

The imbalance induced by inhibition of bone mesenchymal stem cell (BMSC) osteogenic differentiation results in osteoporosis (OP); however, the underlying regulatory mechanism is not completely understood. Long non‑coding RNAs (lncRNAs) serve crucial roles in osteogenic differentiation; therefore, investigating their regulatory role in the process of osteogenic differentiation may identify a promising therapeutic target for OP. The expression of small nucleolar RNA host gene 1 (SNHG1), Dickkopf 1 (DKK1), microRNA (miR)‑101, RUNX family transcription factor 2 (RUNX2), osteopontin (OPN) and osteocalin (OCN) were detected via reverse transcription‑quantitative PCR. The protein expression levels of DKK1, β‑catenin, RUNX2, OPN, OCN, osterix and collagen type I α1 chain were analyzed by performing western blotting. The osteoblastic phenotype was assessed by conducting alkaline phosphatase activity detection and Alizarin Red staining. The interaction between SNHG1 and miR‑101 was validated by bioinformatics and luciferase assays. The regulatory role of SNHG1 in BMSC osteogenic differentiation was assessed. SNHG1 expression was downregulated in a time‑dependent manner during the process of osteogenic differentiation. SNHG1 overexpression inhibited osteogenic differentiation compared with the pcDNA group. The results indicated that SNHG1 and DKK1 directly interacted with miR‑101. Moreover, SNHG1 regulated the Wnt/β‑catenin signaling pathway to inhibit osteogenic differentiation via the miR‑101/DKK1 axis. The present study indicated that lncRNA SNHG1 could attenuate BMSC osteogenic differentiation via the miR‑101/DKK1 axis as a competitive endogenous RNA. Therefore, the present study furthered the current understanding of the potential mechanism underlying lncRNAs in in osteogenic differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Xiang J, Fu HQ, Xu Z, Fan WJ, Liu F and Chen B: lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells . Mol Med Rep 22: 3715-3722, 2020.
APA
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., & Chen, B. (2020). lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells . Molecular Medicine Reports, 22, 3715-3722. https://doi.org/10.3892/mmr.2020.11489
MLA
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., Chen, B."lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells ". Molecular Medicine Reports 22.5 (2020): 3715-3722.
Chicago
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., Chen, B."lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells ". Molecular Medicine Reports 22, no. 5 (2020): 3715-3722. https://doi.org/10.3892/mmr.2020.11489
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang J, Fu HQ, Xu Z, Fan WJ, Liu F and Chen B: lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells . Mol Med Rep 22: 3715-3722, 2020.
APA
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., & Chen, B. (2020). lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells . Molecular Medicine Reports, 22, 3715-3722. https://doi.org/10.3892/mmr.2020.11489
MLA
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., Chen, B."lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells ". Molecular Medicine Reports 22.5 (2020): 3715-3722.
Chicago
Xiang, J., Fu, H., Xu, Z., Fan, W., Liu, F., Chen, B."lncRNA SNHG1 attenuates osteogenic differentiation via the miR‑101/DKK1 axis in bone marrow mesenchymal stem cells ". Molecular Medicine Reports 22, no. 5 (2020): 3715-3722. https://doi.org/10.3892/mmr.2020.11489
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