Effects of local dexmedetomidine administration on the neurotoxicity of ropivacaine for sciatic nerve block in rats

Xue,Xing; Fan,Jun; Ma,Xiaoli; Liu,Yongqiang; Han,Xuena; Leng,Yufang; Yu,Jinjia

doi:10.3892/mmr.2020.11514

Effects of local dexmedetomidine administration on the neurotoxicity of ropivacaine for sciatic nerve block in rats

Authors:
- Xing Xue
- Jun Fan
- Xiaoli Ma
- Yongqiang Liu
- Xuena Han
- Yufang Leng
- Jinjia Yu
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Affiliations: Department of Anesthesiology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Anesthesiology, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China, School of Life Sciences, Central South University, Changsha, Hunan 410083, P.R. China
Published online on: September 17, 2020 https://doi.org/10.3892/mmr.2020.11514
Pages: 4360-4366
Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dexmedetomidine, used as an adjuvant to local anesthetics (LAs), may prolong the duration of peripheral nerve block. However, the effect of dexmedetomidine on the neurotoxicity of LAs is not completely understood. The present study was designed to investigate the efficacy of two doses of dexmedetomidine as an adjuvant to ropivacaine and its protective effect against the neurotoxicity of LAs. Paw withdrawal thermal latency testing was used to detect the sensory blockade. Extensor postural thrust testing was used to detect the motor blockade. The results demonstrated that the addition of dexmedetomidine to ropivacaine prolonged the duration of sensory and motor blockade in a dose‑dependent manner compared with ropivacaine alone. TUNEL staining was performed to examine apoptosis. Western blotting was used to detect the Cleaved caspase‑3 expression levels. The results showed that the addition of dexmedetomidine to ropivacaine decreased the rate of apoptosis and caspase‑3 expression levels in a dose‑dependent manner compared with ropivacaine alone (P<0.05). In addition, the rate of apoptosis and caspase‑3 expression levels were significantly lower in the high‑dose dexmedetomidine group compared with the low‑dose dexmedetomidine group (P<0.05). The results suggested that the addition of dexmedetomidine to ropivacaine for sciatic nerve block in rats not only prolonged the duration of sensory and motor block of the sciatic nerve, but also markedly alleviated ropivacaine‑induced neurotoxicity by decreasing caspase‑3‑dependent sciatic nerve cell apoptosis. Furthermore, the present study indicated that dexmedetomidine was more effective at a dose of 20 µg/kg compared with 6 µg/kg.

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