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Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus

  • Authors:
    • Qing Luo
    • Xue Li
    • Biqi Fu
    • Lu Zhang
    • Le Fang
    • Cheng Qing
    • Yang Guo
    • Zikun Huang
    • Junming Li
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Rheumatology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 710065, P.R. China, Department of Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Blood Transfusion, 521 Hospital of Ordnance Industry, Xi'an, Shanxi 710065, P.R. China, Department of Intensive Care Unit, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 1
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    Published online on: November 2, 2020
       https://doi.org/10.3892/mmr.2020.11639
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Abstract

Circular RNAs (circRNAs) have gained attention due to their performance in disease diagnosis. However, the characteristics of circRNAs in peripheral blood from patients with systemic lupus erythematosus (SLE) remain unknown. Therefore, the aim of the present study was to determine the expression profile and diagnostic potential of circRNAs in peripheral blood from patients with SLE. The global circRNA expression in the peripheral blood of patients with SLE and healthy controls (HCs) was detected using a circRNA microarray. Then, the expression levels of three upregulated circRNAs were selected for further validation by reverse transcription‑quantitative PCR (RT‑qPCR) in a training set. Moreover, the diagnostic value of these circRNAs was assessed by constructing a receiver operating characteristic curve, and then verified in a blind testing set. In total, 1,566 circRNAs were identified to be dysregulated between patients with SLE and HCs (≥2 fold change, P<0.05). Furthermore, the RT‑qPCR results were consistent with the microarray data, in that all three selected circRNAs, hsa_circ_0082688, hsa_circ_0082689 and hsa_circ_0008675, were significantly upregulated in patients with SLE (P<0.05). Results from the training set demonstrated that the combination of hsa_circ_0082688‑hsa_circ_0082689 may provide the most beneficial diagnostic potential. Moreover, the blind test results indicated that the combination model of hsa_circ_0082688‑hsa_circ_0082689 could effectively discriminate between patients with SLE from patients with rheumatoid arthritis and HCs, with a sensitivity of 91.30%, a specificity of 78.57% and an accuracy of 82.28%. Moreover, the combination model of hsa_circ_0082688‑hsa_circ_0082689 + anti‑dsDNA could more effectively discriminated the SLE group from the control groups, with a sensitivity of 95.65%, a specificity of 100.00% and an accuracy of 98.73%. In addition, correlation analysis results suggested that all three circRNAs in patients with SLE did not correlate with the SLE disease activity index. In conclusion, the expression levels of hsa_circ_0082688‑hsa_circ_0082689 may serve as potential biomarkers for SLE diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Q, Li X, Fu B, Zhang L, Fang L, Qing C, Guo Y, Huang Z and Li J: Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus. Mol Med Rep 23: 1, 2021.
APA
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C. ... Li, J. (2021). Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus. Molecular Medicine Reports, 23, 1. https://doi.org/10.3892/mmr.2020.11639
MLA
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C., Guo, Y., Huang, Z., Li, J."Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus". Molecular Medicine Reports 23.1 (2021): 1.
Chicago
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C., Guo, Y., Huang, Z., Li, J."Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus". Molecular Medicine Reports 23, no. 1 (2021): 1. https://doi.org/10.3892/mmr.2020.11639
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Q, Li X, Fu B, Zhang L, Fang L, Qing C, Guo Y, Huang Z and Li J: Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus. Mol Med Rep 23: 1, 2021.
APA
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C. ... Li, J. (2021). Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus. Molecular Medicine Reports, 23, 1. https://doi.org/10.3892/mmr.2020.11639
MLA
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C., Guo, Y., Huang, Z., Li, J."Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus". Molecular Medicine Reports 23.1 (2021): 1.
Chicago
Luo, Q., Li, X., Fu, B., Zhang, L., Fang, L., Qing, C., Guo, Y., Huang, Z., Li, J."Expression profile and diagnostic value of circRNAs in peripheral blood from patients with systemic lupus erythematosus". Molecular Medicine Reports 23, no. 1 (2021): 1. https://doi.org/10.3892/mmr.2020.11639
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