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Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells

  • Authors:
    • Gaili An
    • Feng Lu
    • Shangke Huang
    • Jun Bai
    • Li He
    • Yi Liu
    • Lei Hou
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Oncology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © An et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 30
    |
    Published online on: November 4, 2020
       https://doi.org/10.3892/mmr.2020.11668
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Abstract

Triple‑negative breast cancer (TNBC) is characterized by strong invasiveness, frequent local recurrence and distant metastasis, with poor prognosis. According to tumor angiogenesis theory, tumor cells can obtain blood supply not only by fusing with host blood vessels, but also by constructing a new vascular system through angiogenesis, so as to continuously obtain nutrients and oxygen supply; this is called vasculogenic mimicry (VM). In our previous study, differential expression profiles of miRNAs were examined with gene chip in TNBC and corresponding paracancer tissues, which demonstrated significant up‑regulation of microRNA (miR)‑93. Bioinformatics found that the target genes of miR‑93 were associated with cell proliferation, invasion and migration. The present study investigated the association between miR‑93, epithelial‑to‑mesenchymal transition (EMT) and VM formation in TNBC cell lines. The results indicated that miR‑93 depletion suppressed MDA‑MB‑231 cell viability, invasion and migration (P<0.001). In addition, knockdown of miR‑93 significantly upregulated the expression levels of EMT‑associated genes such as E‑cadherin and occludin, but downregulated the expression levels of vimentin and N‑cadherin in MDA‑MB‑231 cells. VM formation assay showed a significant decrease in microtubule‑forming ability of cells following miR‑93 knockdown, which was associated with the occurrence of EMT, suggesting that miR‑93 may promote the formation of VM via EMT and may be a therapeutic target for the treatment of TNBC.
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Copy and paste a formatted citation
Spandidos Publications style
An G, Lu F, Huang S, Bai J, He L, Liu Y and Hou L: Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells. Mol Med Rep 23: 30, 2021.
APA
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., & Hou, L. (2021). Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells. Molecular Medicine Reports, 23, 30. https://doi.org/10.3892/mmr.2020.11668
MLA
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., Hou, L."Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells". Molecular Medicine Reports 23.1 (2021): 30.
Chicago
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., Hou, L."Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells". Molecular Medicine Reports 23, no. 1 (2021): 30. https://doi.org/10.3892/mmr.2020.11668
Copy and paste a formatted citation
x
Spandidos Publications style
An G, Lu F, Huang S, Bai J, He L, Liu Y and Hou L: Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells. Mol Med Rep 23: 30, 2021.
APA
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., & Hou, L. (2021). Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells. Molecular Medicine Reports, 23, 30. https://doi.org/10.3892/mmr.2020.11668
MLA
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., Hou, L."Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells". Molecular Medicine Reports 23.1 (2021): 30.
Chicago
An, G., Lu, F., Huang, S., Bai, J., He, L., Liu, Y., Hou, L."Effects of miR‑93 on epithelial‑to‑mesenchymal transition and vasculogenic mimicry in triple‑negative breast cancer cells". Molecular Medicine Reports 23, no. 1 (2021): 30. https://doi.org/10.3892/mmr.2020.11668
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