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Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis

  • Authors:
    • Yu Wang
    • Jianying Fu
    • Lili Yang
    • Zhi Liang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology Endoscopy, Linyi Central Hospital, Linyi, Shandong 276400, P.R. China, Department of Anorectal Surgery, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 31
    |
    Published online on: November 5, 2020
       https://doi.org/10.3892/mmr.2020.11669
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Abstract

Colorectal cancer (CRC) is one of the primary causes of cancer‑associated mortality worldwide. However, the potential molecular mechanism of CRC progression remains unknown. Long non‑coding RNA small nucleolar RNA host gene 20 (SNHG20) has been demonstrated to be involved in the development and progression of a variety of tumors, including CRC. However, the involvement of SNHG20 in CRC progression remains unclear. The aim of the present study was to investigate the functional role and molecular mechanism of SNHG20 in CRC progression. In the present study, SNHG20 expression was found to be significantly upregulated in CRC tissues and cell lines. Association analysis indicated that high SNHG20 expression was significantly association with greater tumor size (P=0.014), tumor invasion depth (P=0.019), positive lymph node status (P=0.022), distant metastasis (P=0.017) and advanced tumor node metastasis stage (P=0.038). Loss‑of‑function experiments indicated that SNHG20 knockdown could significantly suppress proliferation, migration and invasion in vitro. Notably, SNHG20 knockdown significantly inhibited tumor growth and lung metastasis in vivo. Bioinformatics analysis and luciferase reporter assays confirmed that microRNA (miR)‑495 was a direct target of SNHG20. Rescue assays indicated that miR‑495 inhibitor reversed the suppressive effects of SNHG20 knockdown on CRC progression. Moreover, STAT3 was identified as a downstream target of miR‑495 in CRC. STAT3 overexpression partially rescued the inhibitory effects of SNHG20 knockdown on CRC progression. Taken together, the results revealed that SNHG20 facilitated CRC progression by regulating STAT3 expression and by sponging miR‑495.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Fu J, Yang L and Liang Z: Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis. Mol Med Rep 23: 31, 2021.
APA
Wang, Y., Fu, J., Yang, L., & Liang, Z. (2021). Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis. Molecular Medicine Reports, 23, 31. https://doi.org/10.3892/mmr.2020.11669
MLA
Wang, Y., Fu, J., Yang, L., Liang, Z."Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis". Molecular Medicine Reports 23.1 (2021): 31.
Chicago
Wang, Y., Fu, J., Yang, L., Liang, Z."Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis". Molecular Medicine Reports 23, no. 1 (2021): 31. https://doi.org/10.3892/mmr.2020.11669
Copy and paste a formatted citation
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Spandidos Publications style
Wang Y, Fu J, Yang L and Liang Z: Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis. Mol Med Rep 23: 31, 2021.
APA
Wang, Y., Fu, J., Yang, L., & Liang, Z. (2021). Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis. Molecular Medicine Reports, 23, 31. https://doi.org/10.3892/mmr.2020.11669
MLA
Wang, Y., Fu, J., Yang, L., Liang, Z."Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis". Molecular Medicine Reports 23.1 (2021): 31.
Chicago
Wang, Y., Fu, J., Yang, L., Liang, Z."Long non‑coding RNA SNHG20 promotes colorectal cancer cell proliferation, migration and invasion via miR‑495/STAT3 axis". Molecular Medicine Reports 23, no. 1 (2021): 31. https://doi.org/10.3892/mmr.2020.11669
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