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Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model

  • Authors:
    • Shun Zhang
    • Lingling Zhuang
    • Qian Liu
    • Xiaolin Yu
    • Qinghua Min
    • Minjie Chen
    • Qi Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Gynecology and Obstetrics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Clinical Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 35
    |
    Published online on: November 9, 2020
       https://doi.org/10.3892/mmr.2020.11673
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Abstract

Endometriosis is closely associated with inflammatory reactions and angiogenesis. Whether PPARγ is a target for the treatment of endometriosis remains unknown. The present study was designed to investigate the impact of a PPARγ agonist (rosiglitazone, RSG) on endometriosis in a rat model and to identify the underlying mechanism. The endometriosis model was established in rats. The pathological state of the endometrium was examined using hematoxylin‑eosin staining. The microstructures of interest were visualized using electron microscopy. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction were used to detect PPARγ and MAT2A expression. VEGF and caspase‑3 expression were investigated using immunohistochemistry. Pathological analysis revealed transparent and red nodules in the model group, and that vasoganglions were present all over the nodules. Endometrial epithelial hyperplasia was observed in the model group, and the shape was columnar. Increased interstitial cell numbers, with compact structure and abundant blood supply, were detected in the model group. Compared with the model group, incomplete epithelial structures with sparse interstitial cells and loose structure were observed in the pathological images from RSG treatment groups. Numerous inflammatory cells and poor blood supply were observed in the endometrial tissues, and the gland was filled mostly with vacuolar cells. Electron microscopy revealed that the tissue structure was integrated. Many vacuoles were formed within the endometrial tissue and the classical morphological changes of apoptotic cells were observed in RSG‑treated groups. Caspase‑3 and PPARγ expression increased and expression of VEGF and MAT2A decreased in RSG‑treated groups. Taken together, these results revealed that RSG impacts the development and progression of endometriosis likely by inhibiting angiogenesis and inducing apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang S, Zhuang L, Liu Q, Yu X, Min Q, Chen M and Chen Q: Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model. Mol Med Rep 23: 35, 2021.
APA
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., & Chen, Q. (2021). Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model. Molecular Medicine Reports, 23, 35. https://doi.org/10.3892/mmr.2020.11673
MLA
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., Chen, Q."Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model". Molecular Medicine Reports 23.1 (2021): 35.
Chicago
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., Chen, Q."Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model". Molecular Medicine Reports 23, no. 1 (2021): 35. https://doi.org/10.3892/mmr.2020.11673
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang S, Zhuang L, Liu Q, Yu X, Min Q, Chen M and Chen Q: Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model. Mol Med Rep 23: 35, 2021.
APA
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., & Chen, Q. (2021). Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model. Molecular Medicine Reports, 23, 35. https://doi.org/10.3892/mmr.2020.11673
MLA
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., Chen, Q."Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model". Molecular Medicine Reports 23.1 (2021): 35.
Chicago
Zhang, S., Zhuang, L., Liu, Q., Yu, X., Min, Q., Chen, M., Chen, Q."Rosiglitazone affects the progression of surgically‑induced endometriosis in a rat model". Molecular Medicine Reports 23, no. 1 (2021): 35. https://doi.org/10.3892/mmr.2020.11673
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