Open Access

Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer

  • Authors:
    • Yusuke Sayama
    • Mika K. Kaneko
    • Yukinari Kato
  • View Affiliations

  • Published online on: November 25, 2020     https://doi.org/10.3892/mmr.2020.11731
  • Article Number: 92
  • Copyright : © Sayama et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Trophoblast cell‑surface antigen 2 (TROP2) is a type I transmembrane glycoprotein that is overexpressed in a number of cancer types, including triple‑negative breast cancer. The current study aimed to develop a highly sensitive and specific monoclonal antibody (mAb) targeting TROP2, which could be used to evaluate TROP2 expression using flow cytometry, western blot analysis and immunohistochemistry by employing the Cell‑Based Immunization and Screening (CBIS) method. The established anti‑TROP2 mAb, TrMab‑6 (mouse IgG2b, κ), detected TROP2 on PA‑tagged TROP2‑overexpressing Chinese hamster ovary‑K1 (CHO/TROP2‑PA) and breast cancer cell lines, including MCF7 and BT‑474 using flow cytometry. Western blot analysis indicated a 40 kDa band in lysates prepared from CHO/TROP2‑PA, MCF7 and BT‑474 cells. Furthermore, TROP2 in 57/61 (93.4%) of the breast cancer specimens was strongly detected using immunohistochemical analysis with TrMab‑6. In conclusion, the current study demonstrated that TrMab‑6 may be a valuable tool for the detection of TROP2 in a wide variety of breast cancer types.
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February-2021
Volume 23 Issue 2

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Spandidos Publications style
Sayama Y, Kaneko MK and Kato Y: Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer. Mol Med Rep 23: 92, 2021
APA
Sayama, Y., Kaneko, M.K., & Kato, Y. (2021). Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer. Molecular Medicine Reports, 23, 92. https://doi.org/10.3892/mmr.2020.11731
MLA
Sayama, Y., Kaneko, M. K., Kato, Y."Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer". Molecular Medicine Reports 23.2 (2021): 92.
Chicago
Sayama, Y., Kaneko, M. K., Kato, Y."Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer". Molecular Medicine Reports 23, no. 2 (2021): 92. https://doi.org/10.3892/mmr.2020.11731