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Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis

  • Authors:
    • Huo-Wang Liu
    • Zhong-Liang Hu
    • Hao Li
    • Qi-Feng Tan
    • Jing Tong
    • Yong-Quan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410007, P.R. China, Department of Otolaryngology-Head and Neck Surgery, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 145
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    Published online on: December 15, 2020
       https://doi.org/10.3892/mmr.2020.11784
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Abstract

The incidence of allergic rhinitis (AR) is increasing worldwide. Human nasal epithelial cells (HNECs) are the key cells in the occurrence of AR. Antisense non-coding RNA in the INK4 locus (ANRIL) was discovered to be involved in the progression of AR. However, the mechanism by which ANRIL mediates the progression of AR remains to be determined. The present study aimed to further explore the mechanism by which ANRIL regulates AR. Thereby, HNECs were treated with IL-13 to mimic AR in vitro. The mRNA expression levels of ANRIL, microRNA (miR)-15a-5p, JAK2, mucin 5AC (MUC5AC), granulocyte-macrophage colony-stimulating factor (GM-CSF) and eotaxin-1, and protein expression levels of JAK2, STAT3 and phosphorylated-STAT3 in HNECs were analyzed using reverse transcription-quantitative PCR and western blotting, respectively. ELISAs were used to detect the secretory levels of inflammatory cytokines and mucin in cell supernatants. In addition, a dual luciferase reporter assay was used to confirm the downstream target of ANRIL and the target gene of miR-15a-5p. The results revealed that the secretory levels of eotaxin-1, GM-CSF and MUC5AC were significantly upregulated by IL-13 in the supernatant of HNECs. The expression levels of ANRIL and JAK2 were also upregulated in IL-13-induced HNECs, while the expression levels of miR-15a-5p were downregulated. In addition, ANRIL was identified to bind to miR-15a-5p. The IL-13-induced upregulation of eotaxin-1, GM-CSF and MUC5AC mRNA expression and secretory levels was significantly inhibited by the genetic knockdown of ANRIL, while the miR-15a-5p inhibitor effectively reversed this effect. JAK2 was also discovered to be directly targeted by miR-15a-5p. The overexpression of JAK2 significantly suppressed the therapeutic effect of miR-15a-5p mimics on IL-13-induced inflammation in vitro. In conclusion, the findings of the present study suggested that the genetic knockdown of ANRIL may suppress the production of inflammatory cytokines and mucin in IL-13-treated HNECs via regulation of the miR-15a-5p/JAK2 axis. Thus, ANRIL may serve as a novel target for AR treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Hu Z, Li H, Tan Q, Tong J and Zhang Y: Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis. Mol Med Rep 23: 145, 2021.
APA
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., & Zhang, Y. (2021). Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis. Molecular Medicine Reports, 23, 145. https://doi.org/10.3892/mmr.2020.11784
MLA
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., Zhang, Y."Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis". Molecular Medicine Reports 23.2 (2021): 145.
Chicago
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., Zhang, Y."Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis". Molecular Medicine Reports 23, no. 2 (2021): 145. https://doi.org/10.3892/mmr.2020.11784
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Hu Z, Li H, Tan Q, Tong J and Zhang Y: Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis. Mol Med Rep 23: 145, 2021.
APA
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., & Zhang, Y. (2021). Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis. Molecular Medicine Reports, 23, 145. https://doi.org/10.3892/mmr.2020.11784
MLA
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., Zhang, Y."Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis". Molecular Medicine Reports 23.2 (2021): 145.
Chicago
Liu, H., Hu, Z., Li, H., Tan, Q., Tong, J., Zhang, Y."Knockdown of lncRNA ANRIL suppresses the production of inflammatory cytokines and mucin 5AC in nasal epithelial cells via the miR-15a-5p/JAK2 axis". Molecular Medicine Reports 23, no. 2 (2021): 145. https://doi.org/10.3892/mmr.2020.11784
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