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9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells

  • Authors:
    • Hyun Mi Kim
    • Bomi Nam
    • Sunil Babu Paudel
    • Joo-Won Nam
    • Ah-Reum Han
    • Hye Gwang Jeong
    • Chang Hyun Jin
  • View Affiliations / Copyright

    Affiliations: Radiation Breeding Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup‑si, Jeollabuk‑do 56212, Republic of Korea, College of Pharmacy, Yeungnam University, Gyengsan‑si, Gyeongsangbuk‑do 38541, Republic of Korea, College of Pharmacy, Chungnam National University, Daejeon, Chungcheongnam‑do 34134, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 181
    |
    Published online on: December 31, 2020
       https://doi.org/10.3892/mmr.2020.11820
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Abstract

Isoegomaketone (IK) is a known component of Perilla frutescens that reportedly exhibits anti‑inflammatory, anti‑cancer and anti‑allergic properties. A novel compound known as 9‑HIK has been isolated from the extract of a radiation mutant P. frutescens var. crispa using supercritical carbon dioxide. In the present study, 9‑HIK induced heme oxygenase‑1 (HO‑1) mRNA expression in RAW264.7 cells, with maximal levels observed 4 h after 9‑HIK treatment. In addition, 9‑HIK inhibited the mRNA and protein expression of pro‑inflammatory mediators, such as IL‑6 and interferon‑β, as well as the production of nitric oxide (NO) in lipopolysaccharide‑stimulated RAW264.7 cells. Furthermore, N‑acetyl‑L‑cysteine, a reactive oxygen species scavenger, inhibited NO production and HO‑1 mRNA expression levels through the nuclear factor erythroid 2‑related factor 2 pathway. Overall, 9‑HIK displayed anti‑inflammatory properties in LPS‑induced RAW264.7 cells via direct suppression of inflammatory mediators and HO‑1 induction.
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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Nam B, Paudel S, Nam J, Han A, Jeong H and Jin C: 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Mol Med Rep 23: 181, 2021.
APA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., & Jin, C. (2021). 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Molecular Medicine Reports, 23, 181. https://doi.org/10.3892/mmr.2020.11820
MLA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23.3 (2021): 181.
Chicago
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23, no. 3 (2021): 181. https://doi.org/10.3892/mmr.2020.11820
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Nam B, Paudel S, Nam J, Han A, Jeong H and Jin C: 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Mol Med Rep 23: 181, 2021.
APA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., & Jin, C. (2021). 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Molecular Medicine Reports, 23, 181. https://doi.org/10.3892/mmr.2020.11820
MLA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23.3 (2021): 181.
Chicago
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23, no. 3 (2021): 181. https://doi.org/10.3892/mmr.2020.11820
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