Open Access

9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells

  • Authors:
    • Hyun Mi Kim
    • Bomi Nam
    • Sunil Babu Paudel
    • Joo-Won Nam
    • Ah-Reum Han
    • Hye Gwang Jeong
    • Chang Hyun Jin
  • View Affiliations

  • Published online on: December 31, 2020     https://doi.org/10.3892/mmr.2020.11820
  • Article Number: 181
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Isoegomaketone (IK) is a known component of Perilla frutescens that reportedly exhibits anti‑inflammatory, anti‑cancer and anti‑allergic properties. A novel compound known as 9‑HIK has been isolated from the extract of a radiation mutant P. frutescens var. crispa using supercritical carbon dioxide. In the present study, 9‑HIK induced heme oxygenase‑1 (HO‑1) mRNA expression in RAW264.7 cells, with maximal levels observed 4 h after 9‑HIK treatment. In addition, 9‑HIK inhibited the mRNA and protein expression of pro‑inflammatory mediators, such as IL‑6 and interferon‑β, as well as the production of nitric oxide (NO) in lipopolysaccharide‑stimulated RAW264.7 cells. Furthermore, N‑acetyl‑L‑cysteine, a reactive oxygen species scavenger, inhibited NO production and HO‑1 mRNA expression levels through the nuclear factor erythroid 2‑related factor 2 pathway. Overall, 9‑HIK displayed anti‑inflammatory properties in LPS‑induced RAW264.7 cells via direct suppression of inflammatory mediators and HO‑1 induction.
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March-2021
Volume 23 Issue 3

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Spandidos Publications style
Kim H, Nam B, Paudel S, Nam J, Han A, Jeong H and Jin C: 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Mol Med Rep 23: 181, 2021
APA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., & Jin, C. (2021). 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells. Molecular Medicine Reports, 23, 181. https://doi.org/10.3892/mmr.2020.11820
MLA
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23.3 (2021): 181.
Chicago
Kim, H., Nam, B., Paudel, S., Nam, J., Han, A., Jeong, H., Jin, C."9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cells". Molecular Medicine Reports 23, no. 3 (2021): 181. https://doi.org/10.3892/mmr.2020.11820