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Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells

  • Authors:
    • Wenyi Fang
    • Peilin Zhao
    • Aling Shen
    • Liya Liu
    • Hongwei Chen
    • Youqin Chen
    • Jun Peng
    • Thomas J. Sferra
    • Senthilkumar Sankararaman
    • Yunfeng Luo
    • Xiao Ke
  • View Affiliations / Copyright

    Affiliations: Spleen and Stomach Research Room, Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350003, P.R. China, Academy of Integrative Medicine, Geriatric Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Department of Pediatrics, Case Western Reserve University School of Medicine, Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 205
    |
    Published online on: January 14, 2021
       https://doi.org/10.3892/mmr.2021.11844
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Abstract

Disruption of the intestinal mucosal barrier integrity is a pathogenic process in inflammatory bowel disease (IBD) development, and is therefore considered a drug discovery target for IBD. The well‑known traditional Chinese formulation Qing Hua Chang Yin (QHCY) has been suggested as a potential therapeutic agent for the treatment of ulcerative colitis. However, the possible underlying molecular mechanisms regarding its therapeutic effect remain unclear. Consequently, the present study investigated the effects of QHCY on lipopolysaccharide (LPS)‑induced loss of intestinal epithelial barrier integrity in vitro using the Caco‑2 cell model of intestinal epithelium. QHCY reversed the LPS‑induced decrease in transepithelial electrical resistance and significantly alleviated the increased fluorescently‑labeled dextran 4 flux caused by LPS. Moreover, QHCY upregulated the mRNA and protein expression levels of occludin, zona occludens‑1 and claudin‑1 in LPS‑exposed Caco‑2 cells. In conclusion, QHCY was able to protect intestinal epithelial barrier integrity following an inflammatory insult; the protective effects of QHCY may be mediated by modulation of the expression of tight junction proteins.
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Copy and paste a formatted citation
Spandidos Publications style
Fang W, Zhao P, Shen A, Liu L, Chen H, Chen Y, Peng J, Sferra TJ, Sankararaman S, Luo Y, Luo Y, et al: Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells. Mol Med Rep 23: 205, 2021.
APA
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y. ... Ke, X. (2021). Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells. Molecular Medicine Reports, 23, 205. https://doi.org/10.3892/mmr.2021.11844
MLA
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y., Peng, J., Sferra, T. J., Sankararaman, S., Luo, Y., Ke, X."Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells". Molecular Medicine Reports 23.3 (2021): 205.
Chicago
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y., Peng, J., Sferra, T. J., Sankararaman, S., Luo, Y., Ke, X."Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells". Molecular Medicine Reports 23, no. 3 (2021): 205. https://doi.org/10.3892/mmr.2021.11844
Copy and paste a formatted citation
x
Spandidos Publications style
Fang W, Zhao P, Shen A, Liu L, Chen H, Chen Y, Peng J, Sferra TJ, Sankararaman S, Luo Y, Luo Y, et al: Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells. Mol Med Rep 23: 205, 2021.
APA
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y. ... Ke, X. (2021). Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells. Molecular Medicine Reports, 23, 205. https://doi.org/10.3892/mmr.2021.11844
MLA
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y., Peng, J., Sferra, T. J., Sankararaman, S., Luo, Y., Ke, X."Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells". Molecular Medicine Reports 23.3 (2021): 205.
Chicago
Fang, W., Zhao, P., Shen, A., Liu, L., Chen, H., Chen, Y., Peng, J., Sferra, T. J., Sankararaman, S., Luo, Y., Ke, X."Effects of Qing Hua Chang Yin on lipopolysaccharide‑induced intestinal epithelial tight junction injury in Caco‑2 cells". Molecular Medicine Reports 23, no. 3 (2021): 205. https://doi.org/10.3892/mmr.2021.11844
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