Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm

  • Authors:
    • Xuezhi Zhang
    • Yilv Wan
    • Jiugeng Feng
    • Meihua Li
    • Zhiqun Jiang
  • View Affiliations

  • Published online on: January 26, 2021     https://doi.org/10.3892/mmr.2021.11869
  • Article Number: 230
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Abstract

Toll‑like receptor (TLR) 2/4 serves an important regulatory role in nerve tissue injury. However, the downstream and potential mechanisms remain to be elucidated. The present study was designed to investigate the roles of the TLR2/4‑major myeloid differentiation response gene 88 (MyD88)‑NF‑κB signaling pathway in the development of intracranial aneurysm. The expression of TLR2, TLR4 and MyD88 in the blood of normal controls and patients with intracranial aneurysm were detected by quantitative PCR and ELISA. Human brain vascular smooth muscle cells were treated by Angiotensin II (Ang II) to evaluate the involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the process. The in vitro experiment was divided into four groups: The control group, an Ang Ⅱ group, an Ang Ⅱ + small interfering (si)RNA control group and an Ang Ⅱ + TLR2‑group. Cell viability, migration, apoptosis and expression of TLR2, TLR4, MyD88, NF‑κB and phosphorylated (p‑)p65 expression were detected. The results demonstrated that the expression of TLR2, TLR4, MyD88 and NF‑κB at mRNA and protein levels in patients with intracranial aneurysm was significantly higher compared with corresponding protein in normal controls (P<0.05). In vitro experiments demonstrated that Ang Ⅱ treatment increased the cell proliferation and migration rate but reduced the apoptotic rate compared with the control (P<0.05). The expression of TLR2, TLR4, MyD88, NF‑κB and p‑p65 was significantly increased in the Ang II group (vs. control; P<0.05). By contrast, TLR2‑short interfering RNA reduced the cell proliferation and migration rate, and reduced the expression of TLR2, TLR4, MyD88, NF‑κB and p‑p65 (vs. Ang Ⅱ + short interfering RNA control; P<0.05). In conclusion, the data of the present study indicated that the TLR2/4‑MyD88‑NF‑κB signaling pathway is involved in the pathogenesis of intracranial aneurysm.
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April-2021
Volume 23 Issue 4

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Spandidos Publications style
Zhang X, Wan Y, Feng J, Li M and Jiang Z: Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm. Mol Med Rep 23: 230, 2021
APA
Zhang, X., Wan, Y., Feng, J., Li, M., & Jiang, Z. (2021). Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm. Molecular Medicine Reports, 23, 230. https://doi.org/10.3892/mmr.2021.11869
MLA
Zhang, X., Wan, Y., Feng, J., Li, M., Jiang, Z."Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm". Molecular Medicine Reports 23.4 (2021): 230.
Chicago
Zhang, X., Wan, Y., Feng, J., Li, M., Jiang, Z."Involvement of TLR2/4‑MyD88‑NF‑κB signaling pathway in the pathogenesis of intracranial aneurysm". Molecular Medicine Reports 23, no. 4 (2021): 230. https://doi.org/10.3892/mmr.2021.11869