Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6ρ0 cells

Jin,Youcai; Luan,Guangxiang; Li,Ji; Wang,Honglun; Wang,Zhenhua; Bai,Bo

doi:10.3892/mmr.2021.11904

Effect of mtDNA depletion from C6 glioma cells and characteristics of the generated C6ρ0 cells

Authors:
- Youcai Jin
- Guangxiang Luan
- Ji Li
- Honglun Wang
- Zhenhua Wang
- Bo Bai
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Affiliations: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, P.R. China, Center for Mitochondria and Healthy Aging, College of Life Sciences, Yantai University, Yantai, Shandong 264005, P.R. China
Published online on: February 8, 2021 https://doi.org/10.3892/mmr.2021.11904
Article Number: 265
Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Malignant tumors of the central nervous system (CNS) are among the types of cancer with the poorest prognosis and glioma is the commonest primary CNS tumor. A mitochondrial DNA (mtDNA)‑depleted cell line C6ρ0 was generated from C6 glioma cells after long‑term exposure to ethidium bromide and 2',3'‑dideoxycytidine in order to determine the effect of mtDNA damage on cell proliferation and pathological changes in glioma cells. Single cell clones were isolated and identified after 42 days of incubation. Repopulated cybrids were formed when the clonal C6ρ0 cells were fused with rat platelets and no difference was observed in their growth in a selective medium without uridine and pyruvate compared with the growth of the parent C6 cells. Disruption of mtDNA resulted in changes in mitochondrial morphology, decreased cell proliferation, reduced intracellular reactive oxygen species and intracellular ATP, along with decreased mtDNA and mitochondrial membrane potential in C6ρ0 cells compared with the C6 cells. Taken together, C6ρ0 cells without mtDNA were established for the first time and their characteristics were compared with parent cells. This C6ρ0 cell line could be used to explore the contribution of mitochondrial dysfunction and mtDNA mutations in the pathogenesis of glioma.

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