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GASC1 promotes glioma progression by enhancing NOTCH1 signaling

  • Authors:
    • Zhengzheng Xiao
    • Xiaoli Yang
    • Zebin Liu
    • Zheng Shao
    • Chaojun  Song
    • Kun Zhang
    • Xiaobin Wang
    • Zhengwei Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Henan Key Laboratory of Cancer Epigenetics, Cancer Institute, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Department of General Practice, The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Spine Tumor Center, Department of Orthopedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai 210011, P.R. China, Department of Urology, Carson International Cancer Centre, Shenzhen University General Hospital and Shenzhen University Clinical Medical Academy Centre, Shenzhen University, Shenzhen, Guangdong 518000, P.R. China, Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
    Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 310
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    Published online on: February 28, 2021
       https://doi.org/10.3892/mmr.2021.11949
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Abstract

Recent studies have reported that gene amplified in squamous cell carcinoma 1 (GASC1) is involved in the progression of several types of cancer. However, whether GASC1 promotes glioma progression remains unknown. Therefore, the present study aimed to investigate the effect of GASC1 exposure on glioma tumorigenesis. The western blot demonstrated that grade III and IV glioma tissues exhibited a higher mRNA and protein expression of GASC1. Moreover, CD133+ U87 or U251 cells from magnetic cell separation exhibited a higher GASC1 expression. Invasion Transwell assay, clonogenic assay and wound healing assay have shown that GASC1 inhibition using a pharmacological inhibitor and specific short hairpin (sh)RNA suppressed the invasive, migratory and tumorsphere forming abilities of primary culture human glioma cells. Furthermore, GASC1‑knockdown decreased notch receptor (Notch) responsive protein hes family bHLH transcription factor 1 (Hes1) signaling. GASC1 inhibition reduced notch receptor 1 (NOTCH1) expression, and a NOTCH1 inhibitor enhanced the effects of GASC1 inhibition on the CD133+ U87 or U251 cell tumorsphere forming ability, while NOTCH1 overexpression abrogated these effects. In addition, the GASC1 inhibitor caffeic acid and/or the NOTCH1 inhibitor DAPT (a γ‑Secretase Inhibitor), efficiently suppressed the human glioma xenograft tumors. Thus, the present results demonstrated the importance of GASC1 in the progression of glioma and identified that GASC1 promotes glioma progression, at least in part, by enhancing NOTCH signaling, suggesting that GASC1/NOTCH1 signaling may be a potential therapeutic target for glioma treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao Z, Yang X, Liu Z, Shao Z, Song C, Zhang K, Wang X and Li Z: GASC1 promotes glioma progression by enhancing NOTCH1 signaling. Mol Med Rep 23: 310, 2021.
APA
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K. ... Li, Z. (2021). GASC1 promotes glioma progression by enhancing NOTCH1 signaling. Molecular Medicine Reports, 23, 310. https://doi.org/10.3892/mmr.2021.11949
MLA
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K., Wang, X., Li, Z."GASC1 promotes glioma progression by enhancing NOTCH1 signaling". Molecular Medicine Reports 23.5 (2021): 310.
Chicago
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K., Wang, X., Li, Z."GASC1 promotes glioma progression by enhancing NOTCH1 signaling". Molecular Medicine Reports 23, no. 5 (2021): 310. https://doi.org/10.3892/mmr.2021.11949
Copy and paste a formatted citation
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Spandidos Publications style
Xiao Z, Yang X, Liu Z, Shao Z, Song C, Zhang K, Wang X and Li Z: GASC1 promotes glioma progression by enhancing NOTCH1 signaling. Mol Med Rep 23: 310, 2021.
APA
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K. ... Li, Z. (2021). GASC1 promotes glioma progression by enhancing NOTCH1 signaling. Molecular Medicine Reports, 23, 310. https://doi.org/10.3892/mmr.2021.11949
MLA
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K., Wang, X., Li, Z."GASC1 promotes glioma progression by enhancing NOTCH1 signaling". Molecular Medicine Reports 23.5 (2021): 310.
Chicago
Xiao, Z., Yang, X., Liu, Z., Shao, Z., Song, C., Zhang, K., Wang, X., Li, Z."GASC1 promotes glioma progression by enhancing NOTCH1 signaling". Molecular Medicine Reports 23, no. 5 (2021): 310. https://doi.org/10.3892/mmr.2021.11949
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