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Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice

  • Authors:
    • Mengmeng Xia
    • Jing Xia
    • Changmin Niu
    • Yanan Zhong
    • Tingting Ge
    • Yue Ding
    • Ying Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
    Copyright: © Xia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 317
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    Published online on: March 3, 2021
       https://doi.org/10.3892/mmr.2021.11956
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Abstract

Gene expression analyses have revealed that there are >2,300 testis-enriched genes in mice, and gene knockout models have shown that a number of them are responsible for male fertility. However, the functions of numerous genes have yet to be clarified. The aim of the present study was to identify the expression pattern of testis-expressed protein 33 (TEX33) in mice and explore the role of TEX33 in male reproduction. Reverse transcription-polymerase chain reaction and western blot assays were used to investigate the mRNA and protein levels of TEX33 in mouse testes during the first wave of spermatogenesis. Immunofluorescence analysis was also performed to identify the cellular and structural localization of TEX33 protein in the testes. Tex33 knockout mice were generated by CRISPR/Cas9 gene-editing. Histological analysis with hematoxylin and eosin or periodic acid-Schiff (PAS) staining, computer-assisted sperm analysis (CASA) and fertility testing, were also carried out to evaluate the effect of TEX33 on mouse spermiogenesis and male reproduction. The results showed that Tex33 mRNA and protein were exclusively expressed in mouse testes and were first detected on postnatal days 21-28 (spermiogenesis phase); their expression then remained into adulthood. Immunofluorescence analysis revealed that TEX33 protein was located in the spermatids and sperm within the seminiferous tubules of the mouse testes, and exhibited specific localization to the acrosome, flagellum and manchette during spermiogenesis. These results suggested that TEX33 may play a role in mouse spermiogenesis. However, Tex33 knockout mice presented no detectable difference in testis-to-body weight ratios when compared with wild-type mice. PAS staining and CASA revealed that spermatogenesis and sperm quality were normal in mice lacking Tex33. In addition, fertility testing suggested that the Tex33 knockout mice had normal reproductive functions. In summary, the findings of the present study indicate that TEX33 is associated with spermiogenesis but is not essential for sperm development and male fertility.
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Copy and paste a formatted citation
Spandidos Publications style
Xia M, Xia J, Niu C, Zhong Y, Ge T, Ding Y and Zheng Y: Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice. Mol Med Rep 23: 317, 2021.
APA
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., & Zheng, Y. (2021). Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice. Molecular Medicine Reports, 23, 317. https://doi.org/10.3892/mmr.2021.11956
MLA
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., Zheng, Y."Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice". Molecular Medicine Reports 23.5 (2021): 317.
Chicago
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., Zheng, Y."Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice". Molecular Medicine Reports 23, no. 5 (2021): 317. https://doi.org/10.3892/mmr.2021.11956
Copy and paste a formatted citation
x
Spandidos Publications style
Xia M, Xia J, Niu C, Zhong Y, Ge T, Ding Y and Zheng Y: Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice. Mol Med Rep 23: 317, 2021.
APA
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., & Zheng, Y. (2021). Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice. Molecular Medicine Reports, 23, 317. https://doi.org/10.3892/mmr.2021.11956
MLA
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., Zheng, Y."Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice". Molecular Medicine Reports 23.5 (2021): 317.
Chicago
Xia, M., Xia, J., Niu, C., Zhong, Y., Ge, T., Ding, Y., Zheng, Y."Testis-expressed protein 33 is not essential for spermiogenesis and fertility in mice". Molecular Medicine Reports 23, no. 5 (2021): 317. https://doi.org/10.3892/mmr.2021.11956
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