Open Access

Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway

  • Authors:
    • Shao Ouyang
    • Wei Chen
    • Zeng Gaofeng
    • Lei Changcheng
    • Tian Guoping
    • Zhu Minyan
    • Liu Yang
    • Yang Min
    • Jiahao Luo
  • View Affiliations

  • Published online on: March 9, 2021     https://doi.org/10.3892/mmr.2021.11977
  • Article Number: 338
  • Copyright: © Ouyang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Pulmonary artery hypertension (PAH) is a disease with high morbidity and mortality. Cyanidin‑3‑O‑β‑glucoside (Cy‑3‑g), a classical anthocyanin, has a variety of biological effects. The present study evaluated whether Cy‑3‑g attenuated PAH, and explored the potential mechanism of action. Rats were injected with monocrotaline (MCT; 60 mg per kg of body weight) and then treated with Cy‑3‑g (200 or 400 mg per kg of body weight) for 4 weeks. Protein expression was determined in vitro in transforming growth factor‑β1 (TGF‑β1)‑mediated human pulmonary arterial smooth muscle cells (SMCs). The results indicated that Cy‑3‑g significantly inhibited the mean pulmonary artery pressure, right ventricular systolic pressure and right ventricular hypertrophy index, as well as vascular remodeling induced by MCT in PAH rats. Further experiments showed that Cy‑3‑g suppressed the expression of pro‑­inflammatory factors and enhanced the levels of anti‑inflammatory factors. Cy‑3‑g blocked oxidative stress and improved vascular endothelial injury. Cy‑3‑g also reduced the proliferation of SMCs. Furthermore, the MCT‑ and TGF‑β1‑induced increase in TGF‑β1, phosphorylated (p)‑p38 mitogen‑activated protein kinase (MAPK) and p‑cAMP‑response element binding protein (CREB) expression was blocked by Cy‑3‑g treatment in vivo and in vitro. These results indicated that Cy‑3‑g could prevent vascular remodeling in PAH via inhibition of the TGF‑β1/p38 MAPK/CREB axis.

Introduction

Pulmonary artery hypertension (PAH) is a disease characterized by pulmonary vascular remodeling and a progressive increase of pulmonary vascular resistance (1). The main manifestations of PAH are pulmonary vasoconstriction, intimal hyperplasia of pulmonary artery, proliferation and hypertrophy of smooth muscle cells (SMCs), which lead to a thickened pulmonary artery wall, narrow lumen and increased pulmonary artery pressure (2,3). It has been reported that PAH could be induced by various pulmonary diseases that cause airflow restriction, destruction of the pulmonary capillary bed, injury of the pulmonary vascular endothelium and infiltration of inflammatory cells in the airways (4,5). PAH is a clinical syndrome with high morbidity and mortality (6). Although the treatment of PAH has made remarkable progress in the past 10 years, its prognosis remains poor (7). Since the exact pathogenesis of PAH is not yet clear, there is no cure for PAH at present. Therefore, it is of great significance to explore an effective treatment for the prevention or alleviation of PAH.

Previous studies have reported that inflammatory responses (8), oxidative stress (9), proliferation of SMCs and endothelial cells (10), and vasodilation (11) are the possible pathological basis of PAH. In a study by Liu et al (12), elevated levels of inflammatory factors, such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), were found in PAH animal models (12). Increased levels of IL-6 are also detected in patients with PAH (13). Oxidative stress may be the main cause of endothelial injury and vascular wall remodeling. Zhang et al (14) reported there is increased malondialdehyde (MDA) and decreased superoxide dismutase (SOD) activity in PAH rats. Therefore, inhibition of chronic pulmonary vascular remodeling induced by inflammation, oxidative stress and cell proliferation is essential to improve the survival rates of patients with PAH.

There is a close association between the formation and development of PAH and members of the transforming growth factor-β (TGF-β) superfamily (15). Abnormally high levels of TGF-β1 have been found in patients with PAH (16). TGF-β1 is a multifunctional channel protein for growth regulation (15). It is one of the most important smooth muscle proliferative cytokines, and exerts physiological effects by activating the p38 mitogen-activated protein kinase (MAPK) signaling pathway (16). The p38 MAPK pathway is the common pathway of proliferation and migration of vascular smooth muscle cells (VSMCs) (17). The p38 MAPK signaling pathway can also mediate inflammation and apoptosis, which is the key target of anti-inflammatory drugs (17). Inhibition of p38 MAPK activation can effectively prevent vascular remodeling of monocrotaline (MCT)-induced PAH (18).

Anthocyanins (ACNs), a type of flavonoids, are widely found in numerous dark-colored foods, such as mulberry and black rice (19). ACNs have a variety of biological effects, which includes anti-inflammatory and anti-oxidative stress properties, thus they can play an inhibitory role in the progression of several chronic diseases (20,21). The anti-inflammatory effects of ACNs have attracted great attention. Herath et al (22) revealed that ACNs inhibited the secretion of the inflammatory factors IL-6 and TNF-α. Previous studies reported the anti-atherosclerotic effect of ACNs, which is associated with their anti-inflammatory and anti-oxidative stress abilities (2325). In addition, it has also been demonstrated that ACNs have the capacity to inhibit the proliferation of SMCs (26) and prevent vascular endothelial cell injury (27). However, few studies have investigated whether ACNs display a protective effect in the development of PAH. In the present study, a rat PAH model was established with the intervention of Cyanidin-3-O-β-glucoside (Cy-3-g; a typical monomer ACN) to investigate whether Cy-3-g could protect against PAH and whether Cy-3-g exerts its inhibitory effect by regulating the TGF-β1 and p38 MAPK signaling pathways.

Materials and methods

Animal model

A total of 60 male Sprague-Dawley rats (age, 8 weeks; weight, 281.3±21.7 g) were obtained from the Animal Center of the University of South China. After 1-week acclimation, rats were randomly assigned to four groups (n=15): A control group (control), a model group (MCT), a low-dose Cy-3-g group (Cy-3-g-L) and a high-dose Cy-3-g group (Cy-3-g-H). On the first day of the experiments, the rats in the control group were injected intraperitoneally with 0.9% normal saline; rats in the MCT, Cy-3-g-L and Cy-3-g-H groups were injected intraperitoneally with MCT (60 mg/kg of body weight). On days 2–28, the rats in the Cy-3-g-L and Cy-3-g-H groups received a gavage of 200 and 400 mg Cy-3-g per kg of body weight, respectively, while the rats in the control and MCT groups received a gavage of normal saline. During the 28 days of animal experiments, rats were housed in a temperature of 21–24°C and a humidity of 45–50°C with 12 h light/dark cycles. All rats were allowed to drink water and eat a normal chow diet (5% fat w/w; Animal Center of the University of South China) ad libitum. The bedding and cages of rats were changed every 2 days, and the weight and food intake of rats was recorded every week. After 28-day treatment, rats were anaesthetized by intraperitoneal injection of 1% pentobarbital sodium (at the dose of 40 mg/kg of body weight; Beyotime Institute of Biotechnology), and then were euthanized using cervical dislocation, with the endpoint of cervical fracture and mydriasis. The procedures used in the present animal experiments were approved by the Animal Care Committee of the University of South China [approval no. SYXK (Xiang): 2005-0001].

Hemodynamic measurements

Right catheterization was performed according to a previous study (10). Briefly, rats were anesthetized with an intraperitoneal injection of 1% sodium pentobarbital, and their right external jugular vein was exposed, which was cut longitudinally and a catheter was inserted into it. The catheter was slowly inserted into the right atrium, right ventricle and pulmonary artery. The catheter was filled with heparin saline and connected to the pressure sensor of PowerLab physiological recorder (ADInstruments). The mean pulmonary artery pressure (mPAP) and right ventricular systolic pressure (RVSP) were recorded.

After being dissected, the hearts of rats were cleared and isolated, and then separated into right ventricle (RV), left ventricle (LV) and septum (S). The weights of the RV, LV and S were recorded and used for the calculation of the right ventricular hypertrophy index (RVHI) according to the formula RVHI = W (RV)/[W (LV)+W (S)].

Morphological analysis of pulmonary arterioles

The upper lobe of the left lung was fixed with 4% paraformaldehyde at 4°C overnight, and cut into 5-µm-thick paraffin sections, which were subjected to hematoxylin and eosin (H&E) staining at room temperature for 5 min. Images were acquired and analyzed by microscopy (Leica Microsystem, Inc.) at magnification, ×200. The medial wall thickness (MT) and medial wall area (MA) were calculated according to the formulae: MT (%) = wall thickness/total vessel thickness ×100%; and MA (%) = wall area/total vessel area ×100%, respectively.

Blood gas analysis

After anesthetization with 1% pentobarbital sodium, the epidermis of the rats was cut off, and blood was collected from the left intercostal space between the third and fourth ribs. In total, 0.5 ml of the collected blood was analyzed with a blood gas analyzer (Instrumentation Laboratory). Partial pressure of arterial oxygen (PaO2), partial pressure of arterial carbon dioxide (PaCO2) and PH values were recorded.

ELISA determination of IL-6, TNF-α and IL-10

According to the manufacturer's protocols, rat ELISA kits of IL-6 (cat. no. EK0412), TNF-α (cat. no. EK0526) and IL-10 (cat. no. EK0418; all purchased from Boster Biological Technology), standards and samples (tissue homogenate) were added to the plate (100 µl/well), and a blank was set as the control. The plate was covered with a membrane and incubated at 37°C for 1 h. After discarding the liquid, buffer A (100 µl/well) was added to the plate, and incubated at 37°C for 1 h. Next, the plate was washed 3 times with PBS with 1% Tween-20 and then incubated with buffer B (100 µl/well) at 37°C for 30 min. After rinsing 5 times, the plate was incubated with the chromogenic substrate 3,3′,5,5′-Tetramethylbenzidine for 10 min in the dark. After terminating the reaction with a terminating solution, the optical density (OD) of each well was measured at 450 nm using a microplate reader (Tecan Spark 10M; Tecan Group, Ltd.).

Reverse transcription-quantitative PCR (RT-qPCR)

Total RNA was extracted from lung tissues using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.). After measuring the concentration, cDNA was synthesized by PrimeScript RT Reagent kit (Takara Bio, Inc.) at 37°C for 15 min. Then, RT-qPCR was conducted with a ViiA™ 7 real-time PCR system (Applied Biosystems; Thermo Fisher Scientific, Inc.) with the SYBR Premix Ex Taq II (Tli RNaseH Plus) kit (Takara Bio, Inc.). The thermocycling conditions were as follows: 95°C for 30 sec; then, 95°C for 5 sec and 60°C for 30 sec (40 cycles). The expression folds were calculated with the 2−ΔΔCq method (28). All primers are listed in Table SI.

Measurement of SOD activity and MDA content

The activity of SOD was measured with a SOD assay kit (cat. no. A001-3-2; Nanjing Jiancheng Bioengineering Institute) using the Water-Soluble Tetrazolium-1 method (29). The OD values were recorded at 450 nm. The content of MDA was detected with a MDA assay kit (cat. no. A003-1-2; Nanjing Jiancheng Bioengineering Institute) using the Thiobarbituric Acid method, as described previously (29), and the OD values were analyzed at 532 nm.

Cell culture and identification

Human pulmonary artery SMCs (HPASMCs) were purchased from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences, and grown in 20% fetal bovine serum-RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) at 37°C, 5% CO2 and 95% humidity. Cells (3–5 passages) were used in subsequent experiments. HPASMCs were pretreated with Cy-3-g (10 or 20 µm) at 37°C for 24 h, followed by treatment with TGF-β1 (8 ng/ml) or P79350 (0.2 µg/kg), an agonist of p38 MAPK, for an additional 24 h at 37°.

HPASMCs were seeded at a density of 1×104 and fixed with 4% paraformaldehyde at 4°C overnight. After blocking with 5% not-fat milk at room temperature for 1 h, the slides were stained with a primary antibody against a-smooth muscle actin (α-SMA; 1:100; cat. no. 19245S; Cell Signaling Technology, Inc.) for 8–12 h at 4°C, followed by incubation with FITC-conjugated goat anti-rabbit IgG (1:200; cat. no. SA00003-2; ProteinTech Group, Inc.) for 1 h at room temperature. Micrographs were captured with a Leica TSC SP5 confocal microscope (Leica Microsystems, Inc.) at magnification, ×200.

Cell viability assay

HPASMCs were seeded in 96-well plates (4×103 cells in 100 µl/well). After treatment with Cy-3-g for 24 h at 37°C, cell viability was detected by a CCK8 assay (Beyotime Institute of Biotechnology), according to the manufacturer's protocols. CCK-8 was added to the cells (10 µl/well) and incubated for 1 h at 37°C. Next, the OD values were observed by a versatile microplate reader (Leica Microsystems, Inc.) at 450 nm.

Western blotting

Briefly, total protein was extracted from lung tissues using RIPA buffer (Beyotime Institute of Biotechnology) and quantified using a BCA protein assay kit (Beyotime Institute of Biotechnology). Next, the extracted proteins (50 µg) were loaded and separated via SDS-PAGE on a 8% gel, and transferred onto PVDF membranes. After blocking with 5% BSA (Shanghai Yeasen Biotechnology Co., Ltd.) at room temperature for 1 h, the membrane was stained with the following primary antibodies: Rabbit anti-α-SMA (1:1,000; cat. no. ab32575), anti-von Willebrand factor (vWF; 1:1,000; cat. no. ab6994), anti-intercellular adhesion molecule-1 (ICAM-1; 1:1,000; cat. no. ab33894), anti-vascular adhesion molecule-1 (VCAM-1; 1:1,000; cat. no. ab134047), anti-smooth muscle 22 (SM22; 1:1,000; cat. no. ab14106), anti-vascular endothelial growth factor (VEGF; 1:1,000; cat. no. ab32152), anti-platelet-derived growth factor (PDGF)-BB (1:1,000; cat. no. ab178409), anti-TGF-β1 (1:1,000; cat. no. ab92486), anti-p38 MAPK (1:1,000; cat. no. ab170099), anti-phosphorylated (p)-p38 MAKP (1:1,000; cat. no. ab4822), anti-cAMP-response element binding protein (CREB; 1:1,000; cat. no. ab32515), anti-p-CREB (1:1,000; cat. no. ab220798) and anti-β-actin (1:2,000; cat. no. 3700S; Cell Signaling Technology, Inc.) overnight at 4°C. Then, the membrane was incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (1:2,000; cat. no. SC-2004; Santa Cruz Biotechnology, Inc.) at 37°C for 1 h. Then, an ECL kit (Thermo Fisher Scientific, Inc.) was used to detect protein expression. Image-Pro Plus 6.0 software (Media Cybernetics, Inc.) was used for densitometry.

Statistical analysis

Data are presented as the mean ± SD. One-way ANOVA was performed for comparison of statistical differences between groups, followed by Tukey's post hoc test, using SPSS version 22.0 software (IBM Corp.). P<0.05 was considered to indicate a statistically significant difference.

Results

Inhibition of Cy-3-g on hemodynamics and morphological characteristics of PAH

After injection of MCT and intervention with Cy-3-g, the PAH animal model was verified by detecting hemodynamic parameters. As expected, compared with those of normal rats, the mPAP, RVSP and RVHI of MCT-induced rats markedly increased (from 25.00±2.582 to 64.25±5.909 mmHg; from 17.50±3.416 to 54.25±5.901 mmHg; and from 0.1975±0.028 to 0.4450±0.040 mmHg, respectively), as shown in Fig. 1A-C. A low dose Cy-3-g (200 mg/kg of body weight) significantly decreased RVSP in the MCT-induced rats, and a high dose of Cy-3-g (400 mg/kg of body weight) reduced mPAP, RVSP and RVHI significantly in the MCT-induced rats (all P<0.05; Fig. 1A-C).

The vascular remodeling parameters were then detected by H&E staining. As depicted in Fig. 1D and E, MCT increased the percentage of MT and MA from 21.39±2.849 to 42.66±1.961% and from 43.75±2.986 to 73.25±4.272%, respectively. The consumption of Cy-3-g exerted an inhibitory effect on the MCT-induced increase in MT and MA, which was most significant in the high dose group.

In addition, blood gas analysis was used for the evaluation of hypoxic status in rats. MCT led to an increase in PaCO2, and induced a reduction in pH and PaO2, whereas Cy-3-g-H consumption inhibited the effects of MCT on PaCO2, pH and PaO2 (all P<0.05; Fig. 1F).

Protective effects of Cy-3-g on inflammatory and oxidative stress conditions in MCT-induced PAH rats

Next, the possible underlying mechanism of Cy-3-g in the inhibition of PAH development was explored. Immunoinflammatory factors play a key role in the formation and vascular remodeling of PAH (30). Therefore, the expression of inflammatory factors was measured by RT-qPCR and ELISA. The results of Fig. 2A demonstrated that MCT significantly elevated the mRNA levels of IL-6 and TNF-α, and decreased the levels of IL-10. However, Cy-3-g treatment reversed MCT-induced changes. Similar effects of MCT and Cy-3-g could be observed on the plasma levels of IL-6, TNF-α and IL-10 (Fig. 2B).

Excessive oxidative stress leads to injury of vascular endothelial cells, which is a risk factor for PAH (8). Thus, the present study evaluated the effects of Cy-3-g on oxidative stress. As shown in Fig. 2C and D, the SOD activity and MAD content of the MCT group increased 0.64- and 1.67-fold than those of the control group, respectively. Compared with that of the MCT group, the SOD activity of the Cy-3-g-L and Cy-3-g-H groups increased by 0.16- and 0.42-fold, respectively, while the MAD content of the Cy-3-g-L and Cy-3-g-H groups decreased by 0.18- and 0.39-fold, respectively. Considering the harmful effects of oxidative stress on vascular function, the expression levels of vWF, ICAM-1 and VCAM-1 were evaluated. The results of Fig. 2E and F indicated that the expression of vWF was reduced, while the levels of ICAM-1 and VCAM-1 were increased significantly after treatment with MCT. Cy-3-g intervention reversed the above changes significantly.

Effects of Cy-3-g on cell proliferation in MCT-induced PAH rats

Vascular remodeling induced by PASMC proliferation is also one of the pathological mechanisms of PAH (10). The present study evaluated the effects of Cy-3-g on PASMC proliferation by detecting the expression of α-SMA and SM22. Fig. 3A showed that, compared with that of the control group, the protein expression of α-SMA and SM22 was enhanced by 5.53- and 7.03-fold, respectively, in the MCT group. As shown in Fig. 3B, the mRNA levels of α-SMA and SM22 were significantly higher in MCT-induced rats than in normal rats (11.67±1.022- and 7.915±0.458-fold, respectively). After Cy-3-g consumption, the protein and mRNA expression of α-SMA and SM22 was reduced significantly. In addition, the expression of the pro-apoptotic protein Bax and the anti-apoptotic protein Bcl2 was measured. Cy-3-g consumption significantly increased the expression of Bax and decreased the expression of Bcl2 (Fig. 3A). VEGF and PDGF are important indicators for evaluating the migration and proliferation of vascular endothelial cells (31). MCT stimulation elevated the expression of VEGF and PDGF-BB, which could be an indicator of migration, proliferation and angiogenesis of vascular endothelial cells. Consumption of Cy-3-g inhibited the MCT-induced increase in VEGF and PDGF-BB expression, which further suggested that Cy-3-g can prevent cell proliferation in MCT-induced PAH rats.

Involvement of the TGF-β1-p38 MAPK-CREB signaling pathway in the Cy-3-g-mediated inhibition of PAH

Since Cy-3-g suppressed the inflammation, oxidative stress and cell proliferation induced by MCT treatment, the possible molecular mechanisms were investigated. The p38 MAPK signaling pathway is a common pathway for the regulation of vascular remodeling (18). Lungs of rats were collected to detect the expression of p38 MAPK and its phosphorylation, as well as the expression of the upstream and downstream factors TGF-β1 and CREB. As depicted in Fig. 4, MCT treatment increased the expression of TGF-β1, which activated the p38 MAPK signaling pathway and promoted the level of p-p38 MAPK, followed by an increase in the phosphorylation of CREB. The activation of p38 MAPK was inhibited significantly by the consumption of Cy-3-g, followed by a decrease in the expression of p-CREB. These findings suggested that Cy-3-g prevented PAH induced by MCT, possibly via the regulation of the TGF-β1-p38 MAPK-CREB signaling pathway.

To confirm the role of the TGF-β1-p38 MAPK-CREB signaling pathway on the inhibition of Cy-3-g on PAH, further in vitro experiments using HPASMCs were performed. The cells were induced by TGF-β1, and the expression of α-SMA was then detected (Fig. 5B). Cy-3-g had no effect on cell viability, as detected by the CCK-8 assay, but had a significant effect on cell proliferation, as shown by the decrease in α-SMA (Fig. 5A). The results of western blotting showed that TGF-β1 stimulation elevated the expression of p-p38 MAPK and p-CREB, which could be blocked by Cy-3-g treatment (Fig. 5C). In order to further validate the aforementioned results, the cells were pretreated with P79350, a common agonist of p38 MAPK. After treatment with P79350, the expression of Bcl2 increased and Bax decreased, thus indicating that the protective effect of Cy-3-g on cell proliferation was reversed by P79350 (Fig. 5D). Moreover, the inhibitory effects of Cy-3-g on the phosphorylation of p38 MAPK and CREB were significantly suppressed. These results indicated that the TGF-β1-p38 MAPK-CREB signaling pathway is involved in the inhibitory action of Cy-3-g in the development of PAH.

Discussion

The model of a single injection of MCT in rats is considered to be one of the most effective animal models for studying the development of PAH. Similar to the development of human PAH, MCT transforms into MCT-pyrrole in the liver of rats, and then damages endothelial cells and increases the infiltration of mononuclear cells, which promotes the development of PAH (32,33). In the present study, after the induction of MCT, significantly increased mPAH, RVSP and RVHI, and thickened pulmonary artery membrane of rats were observed, which indicated the successful establishment of the animal model of PAH. Wang et al (34) has reported that plasma Cy-3-g reached the maximum levels (160.4±46.7 nmol/l) at 0.5 h after the oral gavage of Cy-3-g with 25 mg/kg body weight. Additionally, according to the intervention concentration (range of 10–1,000 mg/kg body weight) in numerous animal studies (35,36), 200 and 400 mg/kg body weight was selected as the intervention concentration. As expected, the inhibitory effects of Cy-3-g on mPAH, RVSP, RVHI and pulmonary artery membrane thickening were observed in MCT-induced rats, which suggested an anti-PAH role of Cy-3-g in MCT-exposed rats.

In the absence of hypoxia, inflammatory cell infiltration and inflammatory destruction in the lung are important factors for pulmonary vascular remodeling and hemodynamic changes (37). During hypoxia, inflammatory factors can further mediate and regulate pulmonary vascular remodeling (37). Therefore, chronic inflammatory response is one of the pathogenic mechanisms of PAH. IL-6 stimulates antigens to promote the proliferation of T cells and the maturation of B cells, and strengthens the local immune response, thereby stimulating the release of systemic inflammatory factors, which promotes the development of PAH (8). Animal experiments have confirmed that overexpression of TNF-α induces the formation and aggravation of PAH, whereas TNF-α receptor-deficient mice failed to induce PAH (38). In addition, abnormal activity of the ras homolog gene/a Rho-associated coiled coil-forming protein kinase signaling pathway promotes the migration and infiltration of inflammatory factors, leading to pulmonary vasoconstriction and formation of PAH (39).

Oxidative stress may be the main cause of endothelial injury and vascular wall remodeling (40). Oxidative stress destroys vascular homeostasis, increases intracellular Ca2+ concentration, and damages proteins, lipids and DNA. In addition, SMCs are vulnerable to superoxide damage, which leads to disorder of cell regulation, resulting in vascular contraction and increased blood pressure caused by smooth muscle contraction (41). Reactive oxygen species promote increased pulmonary vascular responses in newborn piglets and lead to the formation of PAH (42). Zhang et al (14) reported increased MAD content and decreased SOD activity in PAH rats. The present study also observed similar phenomena in MAD content and SOD activity, whereas Cy-3-g consumption reduced MAD content and enhanced SOD activity, which suggested that Cy-3-g has antioxidative effects in PAH model rats. Moreover, it was found that Cy-3-g elevated the downregulated expression of vWF, and reduced the upregulated expression of ICAM-1 and VCAM-1 induced by MCT. Cy-3-g attenuated oxidative stress-induced vascular endothelial damage.

Proliferation of PASMCs, which leads to pulmonary artery contraction and chronic pulmonary artery remodeling, is one of the pathological mechanisms of PAH (10). Therefore, exploring the underlying mechanism of pulmonary artery contraction inhibition, reducing PASMC proliferation and delaying pulmonary vascular remodeling are the key factors to improve the survival rate of patients with PAH. It has been found that inhibition of transmembrane protein 16A expression in pulmonary vessels inhibits the proliferation of VSMCs and thus prevents the development of PAH (43). Classical protein kinase C is closely associated with the abnormal proliferation of PASMCs and the progression of PAH (44). Fan et al (45) indicated that YM155 (sepantronium bromide) inhibited the proliferation of PASMCs and improved pulmonary vascular remodeling in PAH rats. The present study observed the effect of Cy-3-g on the proliferation of PASMCs by detecting the expression of α-SMA, SM22, pro-apoptotic protein Bax and anti-apoptotic protein Bcl2. These findings demonstrated that Cy-3-g notably reduced the expression of α-SMA, SM22 and Bcl2 but elevated the levels of Bax, suggesting the protective effect of Cy-3-g on the proliferation of PASMCs.

Numerous studies have focused on the preventive effects of ACNs on chronic diseases (46). Kong et al (21) demonstrated the properties of ACNs and its metabolite protocatechuic acid, including anti-inflammatory, anti-oxidative stress, inhibition of vascular endothelial cell damage and promotion of cholesterol outflow (17,47). In a study by Zhu et al (48), ACNs increased nitric oxide (NO) release, leading to improved endothelium-dependent vasodilation. A previous epidemiological study also found an increase of ACNs in serum NO secretion (49). Abnormal systolic and diastolic function of pulmonary arterioles is one of the risk factors for PAH (11). NO produced by endothelial cells is a powerful vasodilator (50). It mainly causes relaxation of vascular smooth muscles by activating guanylate cyclase on vascular walls, and can reduce the sensitivity of blood vessels to angiotensin, thereby reducing vascular tension and expanding blood vessels (51). Endothelin has a strong vasoconstrictive effect, and promotes cell proliferation and inflammation (52). The level of endothelin-1 (ET-1) in patients with COPD is significantly increased, and ET-1 receptor antagonists are currently an important drug target in the treatment of PAH (53). ET-1 and NO are two opposite factors that regulate vasodilation. Their imbalance leads to changes in pulmonary vascular endothelial function, especially in the early stage of pulmonary vascular remodeling (54,55).

Some studies have reported on the inhibition of other polyphenols in the progression of PAH. Numerous studies focused on the preventative action of resveratrol on PAH via the activation of silent information regulator 1 (56,57). Rashid et al (58) indicated that polyphenol-rich blackcurrant juice protects chronic bile duct ligation-induced endothelial dysfunction in rats by promoting the production of NO and inhibiting the expression of inflammatory factors. Hua et al (59) indicated that polyphenol extracted from apples decreased the expression of caspase-3 and inducible NO synthase, and inhibited the activation of cation channels, which attenuated the development of pulmonary vasoconstriction. However, the molecular mechanism involved has not been explored in these studies.

The progression of PAH is closely associated with members of the TGF-β superfamily (15). Mutations of bone morphogenetic protein receptor type 2 gene are highly prevalent in patients with PAH, as well as the coding genes of some important molecules in the pathway of the TGF-β superfamily (16). An increasing number of studies have indicated that TGF-β1 is the key mediator of pathogenesis in PAH (60). It has been demonstrated that the increased synthesis and accumulation of TGF-β1 are necessary for PAH progression. Moreover, abnormally elevated levels of TGF-β1 have been found in patients with PAH (16,61). Previous studies used TGF-β1 or BMP factors to interfere with the proliferation of PASMCs (62). TGF-β1 is a multifunctional channel protein, which plays a vital role in growth regulation (16). It promotes the proliferation of VSMCs by changing them from a static and contractile state to a proliferative state via the activation of the p38 MAPK signaling pathway (17). The p38 MAPK signaling pathway regulates cell proliferation, apoptosis, extracellular matrix metabolism and inflammation. Ren et al (63) observed that blocking the p38 MAPK pathway could alleviate the inflammation induced by LPS. In addition, activated p38 MAPK leads to the activation of NF-κB signaling, thereby mediating the inflammatory response (64). CREB is a nuclear transcription enhancer, which regulates cell proliferation, differentiation and survival via phosphorylation (65). The p38 MAPK-CREB signaling pathway can be activated rapidly in hyperglycemia, oxidative stress and inflammatory reactions (66). In the present study, MCT promoted the expression of TGF-β1, and the phosphorylation of p38 MAPK and CREB, while Cy-3-g blocked the activation of the TGF-β1-p38 MAPK-CREB signaling pathway.

In summary, the current study revealed the inhibitory effect of Cy-3-g on hemodynamics and morphological characteristics of PAH in MCT-induced rats. Furthermore, the suppressive effect of Cy-3-g on PAH progression was exerted via the inhibition of vascular remodeling, including inflammatory response, oxidative stress and cell proliferation, possibly through regulation of the TGF-β1-p38 MAPK-CREB signaling pathway. These results demonstrated the protective effects of Cy-3-g on PAH. Cy-3-g may be used as a novel product for the prevention and treatment of PAH.

Supplementary Material

Supporting Data

Acknowledgements

Not applicable.

Funding

The present study was supported by the Scientific Research Projects of Health and Family Planning Commission in Hunan Province of China (grant no. B20180057), the Science and Health Joint Project of the Hunan Provincial Natural Science Foundation of China (grant no. 2018JJ6069), the Scientific Research Projects of Health Commission in Hunan Province of China (grant no. 20201945), the Scientific Research Projects of the University of South China in 2020 (grant no. 202006), the Key Laboratory of Heart Failure Prevention and Treatment in Hengyang (grant no. 2019jh426001) and the Youth Academic Leadership Project of The Second Affiliated Hospital of the University of South China.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

SO and WC developed the overall research plan and oversaw the study. SO carried out all the experiments and wrote the manuscript. ZG, LC and YM contributed to the experiments. TG, ZM and LY analyzed the experimental data and revised manuscript. JL constructed the animal model of pulmary heart disease and part of the cytology experiment, and participated in the data analysis. The manuscript is the original work of all authors, and the final manuscript has been read and approved by all authors.

Ethics approval and consent to participate

The procedures used in the animal experiments were performed in line with the Guide for the Care and Use of Laboratory Animals, and was approved by the Animal Care Committee at the University of South China [approval no. SYXK (Xiang): 2005-0001].

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

References

1 

Vender RL: Chronic hypoxic pulmonary hypertension. Chest. 106:236–243. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Greyson CR: Pathophysiology of right ventricular failure. Crit Care Med. 36 (Suppl 1):S57–S65. 2018. View Article : Google Scholar

3 

Humbert M, Morrell NW, Archer SL, Stenmark KP, MacLean MR, Lang IM, Christman BW, Weir EK, Eickelberg O, Voelkel FN and Rabinovitch M: Cellular and molecular pathobiology of pulmonary arterial hypertension. J Am Coll Cardiol. 43 (Suppl 12):S13–S24. 2014. View Article : Google Scholar

4 

Morrell NW, Adnot S, Archer SL, Dupuis J, Jones PL, MacLean MR, McMurtry IF, Stenmark KR, Thistlethwaite PA, Weissmann N, et al: Cellular and molecular basis of pulmonary arterial hypertension. J Am Coll Cardiol. 54 (Suppl 1):S20–S31. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Tuder RM, Archer SL, Dorfmuller P, Erzurum SC, Guignabert C, Michelakis E, Rabinovitch M, Schermuly R, Stenmark KR and Morrell NW: Relevant issues in the pathology and pathobiology of pulmonary hypertension. J Am Coll Cardiol. 62 (Suppl 25):D4–D12. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Peacock AJ, Murphy NF, McMurray JJ, Caballero L and Stewart S: An epidemiological study of pulmonary arterial hypertension. Eur Respir J. 30:104–109. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Montani D, Chaumais MC, Guignabert C, Günther S, Girerd B, Jaïs X, Algalarrondo V, Price LC, Savale L, Sitbon O, et al: Targeted therapies in pulmonary arterial hypertension. Pharmacol Ther. 141:172–191. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Rabinovitch M, Guignabert C, Humbert M and Nicolls MR: Inflammation and immunity in the pathogenesis of pulmonary arterial hypertension. Circ Res. 115:165–175. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Ahmed LA, Obaid AA, Zaki HF and Agha AM: Role of oxidative stress, inflammation, nitric oxide and transforming growth factor-beta in the protective effect of diosgenin in monocrotaline-induced pulmonary hypertension in rats. Eur J Pharmacol. 740:379–387. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Satoh K, Satoh T, Kikuchi N, Omura J, Kurosawa R, Suzuki K, Sugimura K, Aoki T, Nochioka K, Tatebe S, et al: Basigin mediates pulmonary hypertension by promoting inflammation and vascular smooth muscle cell proliferation. Circ Res. 115:738–750. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Zhang Y and Wu S: Effects of fasudil on pulmonary hypertension in clinical practice. Pulm Pharmacol Ther. 46:54–63. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Liu C, Fang C, Cao G, Liu K, Wang B, Wan Z, Li S and Wu S: Ethyl pyruvate ameliorates monocrotaline-induced pulmonary arterial hypertension in rats. J Cardiovasc Pharmacol. 64:7–15. 2017. View Article : Google Scholar

13 

Balabanian K, Foussat A, Dorfmüller P, Durand-Gasselin I, Capel F, Bouchet-Delbos L, Portier A, Marfaing-Koka A, Krzysiek R, Rimaniol AC, et al: CX(3)C chemokine fractalkine in pulmonary arterial hypertension. Am J Respir Crit Care Med. 165:1419–1425. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Zhang Q, Fan K, Wang P, Yu J, Liu R, Qi H, Sun H and Cao Y: Carvacrol induces the apoptosis of pulmonary artery smooth muscle cells under hypoxia. Eur J Pharmacol. 770:134–146. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Liu Y and Hu CN: Effects of Maher BbeChatain on the expression of transforming growth factor-1 and connective tissue growth factor in diabetic rats. Chin J Geriat Med. 34:6691–6693. 2014.

16 

Joshua DS, Andrew WH and Jackson R: AMP-activated protein kinase inhibits transforming growth factor-β-mediated vascular smooth muscle cell growth: Implications for a Smad-3-dependent mechanism. Am J Physiol Heart Circ Physiol. 309:H1251–H1259. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Kwon IS, Yim JH, Lee HK and Pyo S: Lobaric acid inhibits VCAM-1 expression in TNF-α-etimulated vascular smooth muscle cells via modulation of NF-κB and MAPK signaling pathways. Biomol Ther (Seoul). 24:25–32. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Xu Y, Gu Q and Qu C: Capsaicin pretreatment reversed pulmonary arterial hypertension by alleviating inflammation via p38MAPK pathway. Ex Lung Res. 43:8–18. 2017. View Article : Google Scholar

19 

Ding M, Feng R, Wang SY, Bowman L, Lu Y, Qian Y, Castranova V, Jiang BL and Shi X: Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity. J Biol Chem. 281:17359–17368. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Fang J: Bioavailability of anthocyanins. Drug Metab Rev. 46:508–520. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Kong JM, Chia LS, Goh NK, Chia TF and Brouillard R: Analysis and biological activities of anthocyanins. Phytochemistry. 64:923–933. 2008. View Article : Google Scholar

22 

Herath HM, Takano-Ishikawa Y and Yamaki K: Inhibitory effect of some flavonoids on tumor necrosis factor-alpha production in lipopolysaccharide-stimulated mouse macrophage cell line J774.1. J Med Food. 6:365–370. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Xia XD, Ling WH, Ma J, Xia M, Hou MJ, Wang Q, Zhu HL and Tang ZH: Anthocyanin-rich extract from black rice enhances atherosclerotic slaque stabilization in apolipoprotein E-deficient mice. J Nutr. 136:2220–2225. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Wang DL, Wei X, Yan X, Jin T and Ling WH: Protocatechuic acid, a metabolite of anthocyanins, inhibits monocyte adhesion and reduces atherosclerosis in apolipoprotein E-deficient mice. J Agric Food Chem. 58:12722–12728. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Liu Y, Wang X, Pang J, Zhang HY, Luo J, Qian XY, Chen Q and Ling WH: Attenuation of atherosclerosis by protocatechuic acid via inhibition of M1 and promotion of M2 macrophage polarization. J Agric Food Chem. 67:807–818. 2019. View Article : Google Scholar : PubMed/NCBI

26 

Dell'Agli M, Busciala A and Bosisio E: Vascular effects of wine polyphenols. Cardiovasc Res. 63:593–602. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Xia M, Ling WH, Zhu HL, Ma J, Wang Q, Hou MJ, Tang ZH, Guo HH, Liu C and Ye QY: Anthocyanin attenuates CD40-mediated endothelial cell activation and apoptosis by inhibiting CD40-induced MAPK activation. Atherosclerosis. 202:41–47. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Mao GX, Zheng LD, Cao YB, Chen ZM, Lv YD, Wang YZ, Hu XL, Wang GF and Yan J: Antiaging effect of pine pollen in human diploid fibroblasts and in a mouse model induced by D-Galactose. Oxid Med Cell Longev. 2012:7509632012. View Article : Google Scholar : PubMed/NCBI

30 

Kylhammar D, Hesselstrand R, Nielsen S, Scheele C and Rådegran G: Angiogenic and inflammatory biomarkers for screening and follow-up in patients with pulmonary arterial hypertension. Scand J Rheumatol. 47:319–324. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Appelmann I, Liersch R, Kessler T, Mesters RM and Berdel WE: Angiogenesis inhibition in cancer therapy: Platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) and their receptors: Biological functions and role in malignancy. Recent Results Cancer Res. 180:51–81. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Zhu N, Zhao X, Xiang Y, Ye S, Huang J, Hu W, Lv L and Zeng C: Thymoquinone attenuates monocrotaline-induced pulmonary artery hypertension via inhibiting pulmonary arterial remodeling in rats. Int J Cardiol. 221:587–596. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Maarman G, Lecour S, Butrous G, Thienemann F and Sliwa K: A comprehensive review: The evolution of animal models in pulmonary hypertension research; are we there yet? Pulm Circ. 3:739–756. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Wang D, Zou T, Yang Y, Yan X and Ling W: Cyanidin-3-O-β-glucoside with the aid of its metabolite protocatechuic acid, reduces monocyte infiltration in apolipoprotein E-deficient mice. Biochem. Pharmacol. 7:713–719. 2011.

35 

Yan XR, Wu L, Li B, Meng XJ, Dai HP, Zheng YN and Fu JF: Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis rats. J Surg Res. 199:592–600. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Qian XY, Wang X, Luo J, Liu Y, Pang J, Zhang HY, Xu ZL, Xie JW, Jiang XW and Ling WH: Hypouricemic and nephroprotective roles of anthocyanins in hyperuricemic mice. Food Func. 10:867–878. 2019. View Article : Google Scholar

37 

Joppa P, Petrasova D, Stancak B and Tkacova R: Systemic inflammation in patients with COPD and pulmonary hypertension. Chest. 130:326–333. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Price LC, Wort SJ, Perros F, Dorfmuller P, Huertas A, Montani D, Cohen-Kaminsky S and Humbert M: Inflammation in pulmonary arterial hypertension. Chest. 141:210–221. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Fukumoto Y: Role of the Rho-kinase pathway in pulmonary arterial hypertension. Nihon Yakurigaku Zasshi. 143:178–181. 2014.(In Japanese). View Article : Google Scholar : PubMed/NCBI

40 

Irarrázaval S, Allard J, Campodónico J, Perez D, Strobel P, Vasquez L, Urquiaga I, Echeverria G and Leighton F: Oxidative stress in acute hypobaric hypoxia. High Alt Med Biol. 18:128–134. 2017. View Article : Google Scholar : PubMed/NCBI

41 

Chen J, Wang YX, Dong MQ, Zhang B, Luo Y, Niu W and Li ZC: Reoxygenation reverses hypoxic pulmonary arterial remodeling by inducing smooth muscle cell apoptosis via reactive oxygen species-mediated mitochondrial dysfunction. Am Heart Assoc. 6:e0056022017.

42 

Suresh K, Servinsky L, Jiang HY, Bigham Z, Yun X, Kliment C, Huetsch J, Damarla M and Shimoda LA: Reactive oxygen species induced Ca2+ influx via TRPV4 and microvascular endothelial dysfunction in the SU5416/hypoxia model of pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol. 314:L893–L907. 2018. View Article : Google Scholar : PubMed/NCBI

43 

Zeng JW, Chen BY, Lv XF, Sun L, Zeng XL, Zheng HQ, Du YH, Wang GL, Ma MM and Guan YY: Transmembrane member 16A participates in hydrogen peroxide-induced apoptosis by facilitating mitochondria-dependent pathway in vascular smooth muscle cells. Br J Pharmacol. 175:3669–3684. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Hisayama T, Inomoto M, Hioki Y and Fukui H: Identification of PKC isozymes and effect of knockdown of PKC alpha by antisense oligodeoxynucleotide on iNOS expression via interleukin-1 receptor in vascular smooth muscle cells. Nihon Yakurigaku Zasshi. 114 (Suppl 1):86P–91P. 1999.(In Japanese). View Article : Google Scholar : PubMed/NCBI

45 

Fan Z, Liu B, Zhang S, Liu H, Li Y, Wang D, Liu Y, Li J, Wang N, Liu Y and Zhang B: YM155, a selective survivin inhibitor, reverses chronic hypoxic pulmonary hypertension in rats via upregulating voltage-gated potassium channels. Clin Exp Hypertens. 37:381–387. 2015. View Article : Google Scholar : PubMed/NCBI

46 

Manach C, Mazur A and Scalbert A: Polyphenols and prevention of cardiovascular diseases. Curr Opin Lipidol. 16:77–84. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Xia M, Hou M, Zhu H, Ma J, Tang Z, Wang Q, Li Y, Chi D, Yu X, Zhao T, et al: Anthocyanins induce cholesterol efflux from mouse peritoneal macrophages: The role of the peroxisome proliferator-activated receptor {gamma}-liver X receptor {alpha}-ABCA1 pathway. J Biol Chem. 280:36792–36801. 2005. View Article : Google Scholar : PubMed/NCBI

48 

Zhu Y, Xia M, Yang Y, Liu F, Li Z, Hao YT, Mi MT, Jin T and Ling WH: Purified anthocyanin supplementation improves endothelial function via NO-cGMP activation in hypercholesterolemic individuals. Clin Chem. 57:1524–1533. 2011. View Article : Google Scholar : PubMed/NCBI

49 

Edirisinghe I, Banaszewski K, Cappozzo J, McCarthy D and Burton-Freeman BM: Effect of black currant anthocyanins on the activation of endothelial nitric oxide synthase (eNOS) in vitro in human endothelial cells. J Agric Food Chem. 59:8616–8624. 2013. View Article : Google Scholar

50 

Sun XZ, Tian XY, Wang DW and Li J: Effects of fasudil on hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats. Eur Rev Med Pharmacol Sci. 18:959–964. 2014.PubMed/NCBI

51 

Sim JY: Nitric oxide and pulmonary hypertension. Korean J Anesthesiol. 58:4–14. 2010. View Article : Google Scholar : PubMed/NCBI

52 

Polonio IB, Acencio MM, Pazetti R, Almeida FM, Silva BS, Pereira KA and Souza R: Lodenafil treatment in the monocrotaline model of pulmonary hypertension in rats. J Bras Pneumol. 40:421–424. 2014.(In English, Portuguese). View Article : Google Scholar : PubMed/NCBI

53 

Satwiko MG, Ikeda K, Nakayama K, Yagi K, Hocher B, Hirata K and Emoto N: Targeted activation of endothelin-1 exacerbates hypoxia-induced pulmonary hypertension. Biochem Biophys Res Commun. 465:356–362. 2015. View Article : Google Scholar : PubMed/NCBI

54 

Wardle AJ, Seager MJ, Wardle R, Tulloh RM and Gibbs JS: Guanylate cyclase stimulators for pulmonary hypertension. Cochrane Database Syst Rev. 8:CD0112052016.

55 

Hoeper MM, McLaughlin VV, Dalaan AM, Satoh T and Galie N: Treatment of pulmonary hypertension. Lancet Respir Med. 4:323–336. 2016. View Article : Google Scholar : PubMed/NCBI

56 

Yu L, Tu Y, Jia X, Fang K, Liu L, Wan L, Xiang C, Wang Y, Sun X, Liu T, et al: Resveratrol protects against pulmonary arterial hypertension in rats via activation of silent information regulator 1. Cell Physiol Biochem. 42:55–67. 2017. View Article : Google Scholar : PubMed/NCBI

57 

Zhou S, Li MT, Jia YY, Liu JJ, Wang Q, Tian Z, Liu YT, Chen HZ, Liu DP and Zeng XF: Regulation of cell cycle regulators by SIRT1 contributes to resveratrol-mediated prevention of pulmonary arterial hypertension. Biomed Res Int. 2015:7623492015. View Article : Google Scholar : PubMed/NCBI

58 

Rashid S, Idris-Khodja N, Auger C, Kevers C, Pincemail J, Alhosin M, Boehm N, Oswald-Mammosser M and Schini-Kerth VB: Polyphenol-rich blackcurrant juice prevents endothelial dysfunction in the mesenteric artery of cirrhotic rats with portal hypertension: Role of oxidative stress and the angiotensin system. J Med Food. 21:390–399. 2018. View Article : Google Scholar : PubMed/NCBI

59 

Hua C, Zhao J, Wang H, Chen F, Meng H, Chen L, Zhang Q, Yan J and Yuan L: Apple polyphenol relieves hypoxia-induced pulmonary arterial hypertension via pulmonary endothelium protection and smooth muscle relaxation: In vivo and in vitro studies. Biomed Pharmacother. 107:937–944. 2018. View Article : Google Scholar : PubMed/NCBI

60 

Zhang N, Dong MQ, Luo Y, Zhao F and Li YJ: Danshensu prevents hypoxic pulmonary hypertension in rats by inhibiting the proliferation of pulmonary artery smooth muscle cells via TGF-β-smad3-associated pathway. Eur J Pharmacol. 820:1–7. 2018. View Article : Google Scholar : PubMed/NCBI

61 

Upton PD, Davies RJ, Tajsic T and Morrell NW: Transforming growth factor-β(1) represses bone morphogenetic protein-mediated Smad signaling in pulmonary artery smooth muscle cells via Smad3. Am J Respir Cell Mol Biol. 49:1135–1145. 2013. View Article : Google Scholar : PubMed/NCBI

62 

Sheares KK, Jeffery TK, Long L and Morrell NW: Differential effects of TGF-beta1 and BMP-4 on the hypoxic induction of cyclooxygenase-2 in human pulmonary artery smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 287:L919–L927. 2004. View Article : Google Scholar : PubMed/NCBI

63 

Ren X, Shi Y, Zhao D, Xu M, Li X, Dang Y and Ye X: Naringin protects ultraviolet B-induced skin damage by regulating p38MAPK signal pathway. J Dermatol Sci. 82:106–114. 2016. View Article : Google Scholar : PubMed/NCBI

64 

Chen X, Xiu M, Xing J, Yu S, Min D and Guo F: Lanthanum chloride inhibits LPS mediated expressions of pro-inflammatory cytokines and adhesion molecules in HUVECs: Involvement of NF-κB-Jmjd3 signaling. Cell Physiol Biochem. 42:1713–1724. 2017. View Article : Google Scholar : PubMed/NCBI

65 

Ge J, Zhang Y and Zhang ZZ: Research progress on CREB and the signal transduction by phosphorylation of CREB at serine 133. Cell. 59:675–680. 2009.

66 

Gonzallez GA and Montminy MR: Cyclic AMPs timulatessomatostat in gene transcription by phosphorylation of CREB at serine 133. Cell. 59:675–680. 2009. View Article : Google Scholar

Related Articles

Journal Cover

May-2021
Volume 23 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ouyang S, Chen W, Gaofeng Z, Changcheng L, Guoping T, Minyan Z, Yang L, Min Y and Luo J: Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway. Mol Med Rep 23: 338, 2021.
APA
Ouyang, S., Chen, W., Gaofeng, Z., Changcheng, L., Guoping, T., Minyan, Z. ... Luo, J. (2021). Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway. Molecular Medicine Reports, 23, 338. https://doi.org/10.3892/mmr.2021.11977
MLA
Ouyang, S., Chen, W., Gaofeng, Z., Changcheng, L., Guoping, T., Minyan, Z., Yang, L., Min, Y., Luo, J."Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway". Molecular Medicine Reports 23.5 (2021): 338.
Chicago
Ouyang, S., Chen, W., Gaofeng, Z., Changcheng, L., Guoping, T., Minyan, Z., Yang, L., Min, Y., Luo, J."Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway". Molecular Medicine Reports 23, no. 5 (2021): 338. https://doi.org/10.3892/mmr.2021.11977