Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2021 Volume 23 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2021 Volume 23 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway

  • Authors:
    • Hai‑Wei Li
    • Pan Ying
    • Qi‑Qi Cai
    • Zhi‑Hui Yang
    • Xian‑Long Wu
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 341
    |
    Published online on: March 9, 2021
       https://doi.org/10.3892/mmr.2021.11980
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Melatonin (MT) is an indoleamine hormone that can counteract ischemia‑induced organ injury through its antioxidant effects. The aim of the present study was to investigate the protective effects of exogenous MT against hemorrhagic shock (HS)‑induced hepatic ischemic injury in rats, and the role of the nuclear factor (NF)‑κB signaling pathway in this process. A rat model of HS‑induced hepatic ischemic injury was established. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), tumor necrosis factor (TNF)‑α, interferon (IFN)‑γ, interleukin (IL)‑6 and IL‑1β were measured every 6 h, and the 24‑h survival rate of the rats was analyzed. All surviving rats were sacrificed after 24 h. Pathological changes in the liver and the hepatocyte apoptosis rate were observed by hematoxylin and eosin staining and TUNEL assay, respectively, and the expression levels of NF‑κB p65 and NF‑κB inhibitor α (IκBα) were analyzed by reverse transcription‑quantitative PCR analysis and western blotting. The results demonstrated that the serum levels of ALT, AST, LDH, GDH, TNF‑α, IFN‑γ, IL‑6 and IL‑1β gradually increased after HS compared with those in rats subjected to a sham procedure, but this increase was attenuated by MT. Furthermore, the survival rate of the MT group was significantly higher compared with that of the HS group. The degree of pathological hepatic injury, the hepatocyte apoptosis rate, and the hepatic levels of TNF‑α, IFN‑γ, IL‑6 and IL‑1β were significantly decreased in the MT group compared with the HS group. In addition, the mRNA expression of NF‑κB p65 was significantly decreased and the mRNA expression of IκBα was significantly increased in the MT group compared with the sham group. Furthermore, the NF‑κB p65 protein levels in the MT group were significantly increased in the cytosol but decreased in the nucleus, and the IκBα protein levels were increased while those of phosphorylated IκBα were decreased compared with those in the HS group. Therefore, it may be inferred that exogenous MT alleviates HS‑induced hepatic ischemic injury in rats via the inhibition of NF‑κB activation and IκBα phosphorylation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Cannon JW: Hemorrhagic shock. N Engl J Med. 378:370–379. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Kezelman C: Trauma-informed care and practice in nursing. Aust Nurs Midwifery J. 24:282016.PubMed/NCBI

3 

Slim C, Zaouali MA, Nassrallah H, Ammar HH, Majdoub H, Bouraoui A and Abdennebi HB: Protective potential effects of fucoidan in hepatic cold ischemia-reperfusion injury in rats. Int J Biol Macromol. 155:498–507. 2020. View Article : Google Scholar : PubMed/NCBI

4 

Li Y, Gao M, Xu LN, Yin LH, Qi Y and Peng JY: MicroRNA-142-3p attenuates hepatic ischemia/reperfusion injury via targeting of myristoylated alanine-rich C-kinase substrate. Pharmacol Res. 156:1047832020. View Article : Google Scholar : PubMed/NCBI

5 

Ibrahim SG, El-Emam SZ, Mohamed EA and Abd Ellah MF: Dimethyl fumarate and curcumin attenuate hepatic ischemia/reperfusion injury via Nrf2/HO-1 activation and anti-inflammatory properties. Int Immunopharmacol. 80:1061312020. View Article : Google Scholar : PubMed/NCBI

6 

Fliegauf M and Grimbacher B: Nuclear factor κB mutations in human subjects: The devil is in the details. J Allergy Clin Immunol. 142:1062–1065. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Xiao Y, Huang J, Xu J, Zeng L, Tian J, Lou Y, Liu Y, Hu B, Tong F and Shen R: Targeted delivery of puerarin/glycyrrhetinic acid-PEG-PBLA complex attenuated liver ischemia/reperfusion injury via modulating Toll-like receptor 4/nuclear factor-κB pathway. Ther Deliv. 9:245–255. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Amaral FGD and Cipolla-Neto J: A brief review about melatonin, a pineal hormone. Arch Endocrinol Metab. 62:472–479. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Carrascal L, Nunez-Abades P, Ayala A and Cano M: Role of melatonin in the inflammatory process and its therapeutic potential. Curr Pharm Des. 24:1563–1588. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Tang YL, Sun X, Huang LB, Liu XJ, Qin G, Wang LN, Zhang XL, Ke ZY, Luo JS, Liang C, et al: Melatonin inhibits MLL-rearranged leukemia via RBFOX3/hTERT and NF-κB/COX-2 signaling pathways. Cancer Lett. 443:167–178. 2019. View Article : Google Scholar : PubMed/NCBI

11 

https://www.nc3rs.org.uk/arrive-guidelines

12 

ATLS Subcommittee: American College of Surgeons' Committee on Trauma; International ATLS working group, . Advanced trauma life support (ATLS(R)): The ninth edition. J Trauma Acute Care Surg. 74:1363–1366. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Jiang H, Liu J, Xu Z and Zheng C: Efficacy of different fluid resuscitation methods on coagulation function of rats with traumatic hemorrhagic shock. J Surg Res. 260:259–266. 2020. View Article : Google Scholar : PubMed/NCBI

14 

Suzuki S, Nakamura S, Koizumi T, Sakaguchi S, Baba S, Muro H and Fujise Y: The beneficial effect of a prostaglandin I2 analog on ischemic rat liver. Transplantation. 52:979–983. 1991. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Behrends M, Martinez-Palli G, Niemann CU, Cohen S, Ramachandran R and Hirose R: Acute hyperglycemia worsens hepatic ischemia/reperfusion injury in rats. J Gastrointest Surg. 14:528–535. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Sommer JL, El-Gabalawy R, Taillieu T, Afifi TO and Carleton RN: Associations between trauma exposure and physical conditions among public safety personnel. Can J Psychiatry. 12:548–558. 2020. View Article : Google Scholar

18 

Türedi S, Şahin A, Akça M, Demir S, Köse GDR, Çekiç AB, Yıldırım M, Yuluğ E, Menteşe A, Türkmen S and Acar S: Ischemia-modified albumin and the IMA/albumin ratio in the diagnosis and staging of hemorrhagic shock: A randomized controlled experimental study. Ulus Travma Acil Cerrahi Derg. 26:153–162. 2020.PubMed/NCBI

19 

Kyriakopoulos G, Tsaroucha AK, Valsami G, Lambropoulou M, Kostomitsopoulos N, Christodoulou E, Kakazanis Z, Anagnostopoulos C, Tsalikidis C and Simopoulos CE: Silibinin improves TNF-α and M30 expression and histological parameters in rat kidneys after hepatic ischemia/reperfusion. J Invest Surg. 31:201–209. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Ye Y, Wang W, Zhang W, Peng Y, Liu Y, Yu S, Chen Q, Geng L, Zhou L, Xie H, et al: Galectin-1 attenuates hepatic ischemia reperfusion injury in mice. Int Immunopharmacol. 77:1059972019. View Article : Google Scholar : PubMed/NCBI

21 

Zhang J, Yan X, Tian Y, Li W, Wang H, Li Q, Li Y, Li Z and Wu T: Synthesis of a new water-soluble melatonin derivative with low toxicity and a strong effect on sleep aid. ACS Omega. 5:6494–6499. 2020. View Article : Google Scholar : PubMed/NCBI

22 

Pranil T, Moongngarm A and Loypimai P: Influence of pH, temperature, and light on the stability of melatonin in aqueous solutions and fruit juices. Heliyon. 6:e036482020. View Article : Google Scholar : PubMed/NCBI

23 

Ullah U, Badshah H, Malik Z, Uddin Z, Alam M, Sarwar S, Aman A, Khan AU and Shah FA: Hepatoprotective effects of melatonin and celecoxib against ethanol-induced hepatotoxicity in rats. Immunopharmacol Immunotoxicol. 42:255–263. 2020. View Article : Google Scholar : PubMed/NCBI

24 

Yu HY, Meng LF, Lu XH, Liu LH, Ci X and Zhuo Z: Protective effect of miR-146 against kidney injury in diabetic nephropathy rats through mediating the NF-κB signaling pathway. Eur Rev Med Pharmacol Sci. 24:3215–3222. 2020.PubMed/NCBI

25 

Jia Y, He W, Zhang H, He L, Wang Y, Zhang T, Peng J, Sun P and Qian Y: Morusin ameliorates IL-1β-induced chondrocyte inflammation and osteoarthritis via NF-κB signal pathway. Drug Des Devel Ther. 14:1227–1240. 2020. View Article : Google Scholar : PubMed/NCBI

26 

Feng Y, Duan T, Du Y, Jin S, Wang M, Cui J and Wang RF: LRRC25 functions as an inhibitor of NF-κB signaling pathway by promoting p65/RelA for autophagic degradation. Sci Rep. 7:134482017. View Article : Google Scholar : PubMed/NCBI

27 

Boisson B, Puel A, Picard C and Casanova JL: Human IκBα gain of function: A severe and syndromic immunodeficiency. J Clin Immunol. 37:397–412. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Rahimova N, Babazada H, Higuchi Y, Yamashita F and Hashida M: Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IκBα in living cells. Biochim Biophys Acta Mol Cell Res. 1866:190–198. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Kanan T, Kanan D, Erol I, Yazdi S, Stein M and Durdagi S: Targeting the NF-κB/IκBα complex via fragment-based E-Pharmacophore virtual screening and binary QSAR models. J Mol Graph Model. 86:264–277. 2019. View Article : Google Scholar : PubMed/NCBI

30 

Shi CX, Qi QH, Xu J and Zhao WW: Protective effect of magnesium sulfate on cranial nerves in preeclampsia rats through NF-κB/ICAM-1 pathway. Eur Rev Med Pharmacol Sci. 24:2785–2794. 2020.PubMed/NCBI

31 

Gu H, Shen Q, Mei D, Yang Y, Wei R and Ni M: Melatonin inhibits TE-1 esophageal cancer cells metastasis by suppressing the NF-κB signaling pathway and decreasing MMP-9. Ann Clin Lab Sci. 50:65–72. 2020.PubMed/NCBI

32 

Chen F, Jiang G, Liu H, Li Z, Pei Y, Wang H, Pan H, Cui H, Long J, Wang J and Zheng Z: Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop. Bone Res. 8:102020. View Article : Google Scholar : PubMed/NCBI

33 

Tiong YL, Ng KY, Koh RY, Ponnudurai G and Chye SM: Melatonin prevents oxidative stress-induced mitochondrial dysfunction and apoptosis in high glucose-treated schwann cells via upregulation of Bcl2, NF-κB, mTOR, wnt signalling pathways. Antioxidants (Basel). 8:1982019. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li HW, Ying P, Cai QQ, Yang ZH and Wu XL: Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway. Mol Med Rep 23: 341, 2021.
APA
Li, H., Ying, P., Cai, Q., Yang, Z., & Wu, X. (2021). Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway. Molecular Medicine Reports, 23, 341. https://doi.org/10.3892/mmr.2021.11980
MLA
Li, H., Ying, P., Cai, Q., Yang, Z., Wu, X."Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway". Molecular Medicine Reports 23.5 (2021): 341.
Chicago
Li, H., Ying, P., Cai, Q., Yang, Z., Wu, X."Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway". Molecular Medicine Reports 23, no. 5 (2021): 341. https://doi.org/10.3892/mmr.2021.11980
Copy and paste a formatted citation
x
Spandidos Publications style
Li HW, Ying P, Cai QQ, Yang ZH and Wu XL: Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway. Mol Med Rep 23: 341, 2021.
APA
Li, H., Ying, P., Cai, Q., Yang, Z., & Wu, X. (2021). Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway. Molecular Medicine Reports, 23, 341. https://doi.org/10.3892/mmr.2021.11980
MLA
Li, H., Ying, P., Cai, Q., Yang, Z., Wu, X."Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway". Molecular Medicine Reports 23.5 (2021): 341.
Chicago
Li, H., Ying, P., Cai, Q., Yang, Z., Wu, X."Exogenous melatonin alleviates hemorrhagic shock‑induced hepatic ischemic injury in rats by inhibiting the NF‑κB/IκBα signaling pathway". Molecular Medicine Reports 23, no. 5 (2021): 341. https://doi.org/10.3892/mmr.2021.11980
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team